An Unexpected Alteration Colonic Mucus Appearance in the Constipation Model via an Intestinal Microenvironment

Abstract

Due to the lack of research between the inner layers in the structure of colonic mucous and the metabolism of fatty acid in the constipation model, we aim to determine the changes in the mucous phenotype of the colonic glycocalyx and the microbial community structure following treatment with Rhubarb extract in our research. The constipation and treatment models are generated using adult male C57BL/6N mice. We perform light microscopy and transmission electron microscopy (TEM) to detect a Muc2-rich inner mucus layer attached to mice colon under different conditions. In addition, 16S rDNA sequencing is performed to examine the intestinal flora. According to TEM images, we demonstrate that Rhubarb can promote mucin secretion and find direct evidence of dendritic structure-linked mucus structures with its assembly into a lamellar network in a pore size distribution in the isolated colon section. Moreover, the diversity of intestinal flora has noticeable changes in constipated mice. The present study characterizes a dendritic structure and persistent cross-links have significant changes accompanied by the alteration of intestinal flora in feces in models of constipation and pretreatment with Rhubarb extract.

Rhubarb extract rebuilding the mucus homeostasis and regulating mucin-associated flora to relieve constipation

“…54,55 Our previous study demonstrated that the transmembrane mucins became significantly shorter and thicker in the constipation mice, confirming the existence of a strong relationship between mucin and intestinal flora. 5 Several investigations have found that adherent bacterial communities embed themselves in a complex extracellular matrix to gain a competitive advantage, a process called biofilm formation. 56 Bacteria involved in biofilm production prevail in almost all ecosystems, both in terms of numbers and metabolism.…”

“…In addition, the physiological parameters of feces, including their shape, color, consistency, and water content, were investigated as well. 5 In order to directly evaluate colon status, a colonoscopy was performed on experimental mice utilizing a highresolution mouse video endoscopic system (Miniscope, Biotimestech, China). After performing the standard intestinal preparation, a full-length colonoscopy was performed on the mice after being sedated with isoflurane.…”

“…In addition, the physiological parameters of feces, including their shape, color, consistency, and water content, were investigated as well. 5…”

“…The findings corroborated previous research. 5 A substantial and expanding amount of research has demonstrated that intestinal mucus is made up of the major gel-forming glycoprotein muc2 from the mucin family. 8 Distract tendency genes such as muc13 were further depicted in Figure 3(B) and Supplementary Figure 1(F).…”

“…Notably, we have established that the level of mucin has markedly decreased in the constipation model and Rb can act as a promoter of mucus secretion to alleviate constipation. [4][5][6] The mucus in the mammalian body provides a protective and selective barrier and a lubricating coating over a humid mucosal surface. 7,8 The mucus serves as a buffer between vulnerable epithelial surfaces and the outside environment.…”