An unconventional antimicrobial protein histone from freshwater prawn Macrobrachium rosenbergii: Analysis of immune properties

Arockiaraj, Jesu; Gnanam, Annie J.; Kumaresan, Venkatesh; Palanisamy, R.; Bhatt, Prasanth; Thirumalai, Muthukumaresan Kuppusamy; Roy, Arpita; Pasupuleti, Mukesh; Krishnaveni, M.

doi:10.1016/j.fsi.2013.08.018

Cited by 47 publications

(26 citation statements)

References 56 publications

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“…This H2A completely inhibits the growth of M. luteus at a concentration of 4.5 M, with a mixture of H2B and H4 achieving the same level of killing at 3 M (Patat et al, 2004). A study of recombinant H2A from the freshwater prawn, Macrobrachium rosenbergii, has now confirmed that this histone has antibacterial effects against both Gram-negative and Gram-positive bacteria (Arockiaraj et al, 2013a). Molecular analysis of its cDNA has demonstrated that it has a predicted molecular mass of 15 kDa with a very high sequence identity (99%) to other known histone H2As, including that from P. monodon (Arockiaraj et al, 2013a).…”

Section: Antimicrobial Activities Of Non-immune Proteinsmentioning

confidence: 88%

Antimicrobial proteins: From old proteins, new tricks

Smith

Dyrynda

2015

Molecular Immunology

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This review describes the main types of antimicrobial peptides (AMPs) synthesised by crustaceans, primarily those identified in shrimp, crayfish, crab and lobster. It includes an overview of their range of microbicidal activities and the current landscape of our understanding of their gene expression patterns in different body tissues. It further summarises how their expression might change following various types of immune challenges. The review further considers proteins or protein fragments from crustaceans that have antimicrobial properties but are more usually associated with other biological functions, or are derived from such proteins. It discusses how these unconventional AMPs might be generated at, or delivered to, sites of infection and how they might contribute to crustacean host defence in vivo. It also highlights recent work that is starting to reveal the extent of multi-functionality displayed by some decapod AMPs, particularly their participation in other aspects of host protection. Examples of such activities include proteinase inhibition, phagocytosis, antiviral activity and haematopoiesis.

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Section: Antimicrobial Activities Of Non-immune Proteinsmentioning

confidence: 88%

Antimicrobial proteins: From old proteins, new tricks

Smith

Dyrynda

2015

Molecular Immunology

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“…rosenbergii were injected with crude viral sample [white spot syndrome baculovirus (WSBV) and M. rosenbergii nodovirus (MrNV)] and bacteria (A. hydrophila and V. harveyi) (Arockiaraj et al 2013d). Viral-infected prawn tail tissue was homogenized in sterile 2% NaCl solution and injected to the prawn as reported in our earlier study (Arockiaraj et al 2014).…”

Section: Pathogen Injection and Sample Collectionmentioning

confidence: 99%

A prawn core histone 4: Derivation of N- and C-terminal peptides and their antimicrobial properties, molecular characterization and mRNA transcription

Chaurasia

Palanisamy

Bhatt

et al. 2015

Microbiological Research

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This study investigates the complete molecular characterization including bioinformatics characterization, gene expression, synthesis of N and C terminal peptides and their antimicrobial activity of the core histone 4 (H4) from freshwater giant prawn Macrobrachium rosenbergii (Mr). A cDNA encoding MrH4 was identified from the constructed cDNA library of M. rosenbergii during screening and the sequence was obtained using internal sequencing primers. The MrH4 coding region possesses a polypeptide of 103 amino acids with a calculated molecular weight of 11kDa and an isoelectric point of 11.5. The bioinformatics analysis showed that the MrH4 polypeptide contains a H4 signature at (15)GAKRH(19). Multiple sequence alignment of MrH4 showed that the N-terminal (21-42) and C-terminal (87-101) antimicrobial peptide regions and the pentapeptide or H4 signature (15-19) are highly conserved including in humans. The phylogenetic tree formed two separate clades of vertebrate and invertebrate H4, wherein MrH4 was located within the arthropod monophyletic clade of invertebrate H4 groups. Three-dimensional model of MrH4 was established using I-TASSER program and the model was validated using Ramachandran plot analysis. Schiffer-Edmundson helical wheel modeling was used to predict the helix propensity of N (21-42) and C (87-101) terminal derived Mr peptides. The highest gene expression was observed in gills and is induced by viral [white spot syndrome baculovirus (WSBV) and M. rosenbergii nodovirus (MrNV)] and bacterial (Aeromonas hydrophila and Vibrio harveyi) infections. The N and C terminal peptides were synthesized and their antimicrobial and hemolytic properties were examined. Both peptides showed activity against the tested Gram negative and Gram positive bacteria; however, the highest activity was noticed against Gram negative bacteria. Among the two peptides used in this study, C-terminal peptide yielded better results than the N-terminal peptide. Therefore, C terminal peptide can be recommended for the development of an antimicrobial agent.

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“…In our earlier studies [32,33] we have reported the construction of disease challenge model between prawn and pathogenic microbes. Briefly, WSBV and MrNV infected prawn tail tissue was homogenized in 2% NaCl solution and injected to the prawn.…”

Section: Construction Of Disease Challenge Model and Collection Of Timentioning

confidence: 99%

Molecular profiles and pathogen-induced transcriptional responses of prawn B cell lymphoma-2 related ovarian killer protein (BOK)

Chaurasia

Palanisamy

Harikrishnan³

et al. 2015

Fish & Shellfish Immunology

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An unconventional antimicrobial protein histone from freshwater prawn Macrobrachium rosenbergii: Analysis of immune properties

Cited by 47 publications

References 56 publications

Antimicrobial proteins: From old proteins, new tricks

Antimicrobial proteins: From old proteins, new tricks

A prawn core histone 4: Derivation of N- and C-terminal peptides and their antimicrobial properties, molecular characterization and mRNA transcription

Molecular profiles and pathogen-induced transcriptional responses of prawn B cell lymphoma-2 related ovarian killer protein (BOK)

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