An unbiased seed-based RNAi selection screen identifies small RNAs that inhibit androgen signaling and prostate cancer cell growth

Corbin, Joshua M.; Georgescu, Constantin; Wang, Lin; Wren, Jonathan D.; Bieniasz, Magdalena; Xu, Chao; Asch, Adam S.; Echevarría, Maria J. Ruiz

doi:10.1016/j.omtn.2023.06.021

Cited by 2 publications

(6 citation statements)

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“…To assess the safety and efficacy of our nanoparticles, we elected to further study the LNPs, as they contained an siRNA payload that has demonstrated efficacy against prostate cancer cells. 33 siUG is an siRNA known to inhibit the growth and viability of prostate cancer cells through RNA interference of androgen receptor coregulatory and essential gene networks. As a control, we used LNPs containing a nontarget siRNA (siNT).…”

Section: Nano Letters Pubsacsorg/nanolettmentioning

confidence: 99%

“…We have previously demonstrated that 22Rv1 prostate cancer cells transfected with the siUGUUUGC siRNA, using commercially available transfection reagents, showed a decreased proliferation rate and a decreased viability. 33 Following a similar protocol, we treated 22Rv1 cells with comparable amounts of LNPs loaded with 15 nM siU-GUUUGC (siUG-LNPs) or with 15 nM siNT control (siNT-LNPs) and determined the cell growth rate and viability (Figure 5c,d). We observed that 22Rv1 cells dosed with siUG-LNPs showed significantly lower confluency, used as a proxy for growth rate, than the cells dosed with siNT-LNPs (p < 0.05), indicating that the LNPs can deliver functional siRNAs to the cells (Figure 5c, Table S15, and Figure S11).…”

Section: Nano Letters Pubsacsorg/nanolettmentioning

confidence: 99%

“…Free siRNA and blank LNPs were used as controls. The concentration of the siRNA encapsulated into LNPs was selected on the basis of our published data using the Dharmacon transfection protocol 33 and through our previous experiments determining LNP uptake. We noted that our siRNA-encapsulating LNPs had no significant effect on the viability in the selected macrophage cell line (p > 0.05) (Figure 5e and Table S17).…”

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confidence: 99%

“…Next, we demonstrated that the organic nanoparticle formulations synthesized by the Ender3 setup could be used in cell culture experiments. To assess the safety and efficacy of our nanoparticles, we elected to further study the LNPs, as they contained an siRNA payload that has demonstrated efficacy against prostate cancer cells . siUG is an siRNA known to inhibit the growth and viability of prostate cancer cells through RNA interference of androgen receptor coregulatory and essential gene networks.…”

mentioning

confidence: 99%

“…Next, we studied the efficacy of the LNPs to deliver functional siRNA into cells grown in culture. We have previously demonstrated that 22Rv1 prostate cancer cells transfected with the siUGUUUGC siRNA, using commercially available transfection reagents, showed a decreased proliferation rate and a decreased viability …”

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confidence: 99%

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Toward the Scalable, Rapid, Reproducible, and Cost-Effective Synthesis of Personalized Nanomedicines at the Point of Care

Young,

He,

Joo

et al. 2024

Nano Lett.

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Organic nanoparticles are used in nanomedicine, including for cancer treatment and some types of COVID-19 vaccines. Here, we demonstrate the scalable, rapid, reproducible, and cost-effective synthesis of three model organic nanoparticle formulations relevant to nanomedicine applications. We employed a custom-made, low-cost fluid mixer device constructed from a commercially available three-dimensional printer. We investigated how systematically changing aqueous and organic volumetric flow rate ratios determined liposome, polymer nanoparticle, and solid lipid nanoparticle sizes, size distributions, and payload encapsulation efficiencies. By manipulating inlet volumes, we synthesized organic nanoparticles with encapsulation efficiencies approaching 100% for RNA-based payloads. The synthesized organic nanoparticles were safe and effective at the cell culture level, as demonstrated by various assays. Such cost-effective synthesis approaches could potentially increase the accessibility to clinically relevant organic nanoparticle formulations for personalized nanomedicine applications at the point of care, especially in nonhospital and low-resource settings.

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Section: Nano Letters Pubsacsorg/nanolettmentioning

confidence: 99%

Section: Nano Letters Pubsacsorg/nanolettmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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