An UMAMIT-GTR transporter cascade controls glucosinolate seed loading in Arabidopsis

Sanden, Niels Christian Holm; Kanstrup, Christa; Crocoll, Christoph; Schulz, Alexander; Nour-Eldin, Hussam Hassan; Halkier, Barbara Ann; Xu, Deyang

doi:10.1038/s41477-023-01598-4

Cited by 5 publications

(8 citation statements)

References 32 publications

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“…In this study we also showed the presence of the C8 8-methyl-sulfinyl-octyl-GSL in SCE (Figure 7B) that is not usually detected in camelina seeds. The contrasting GSL accumulation pattern between these two Brassicaceae species could be partially due to the absence of benzoylated and sinapoylated GSLs in camelina seeds, whereas they accumulate at high level in Arabidopsis embryos (Kliebenstein et al, 2007; Lee et al, 2012; Bussell et al, 2014; Sanden et al, 2023). In particular, sinapoyl- and benzoyl-GSLs could be less toxic than non-decorated GSLs or could be transported into the vacuole, thus facilitating their accumulation in the embryo.…”

Section: Discussionmentioning

confidence: 96%

“…PAs are specifically synthetized in the seed coat, where they are accumulated at high concentrations at early Arabidopsis seed developmental stages, starting from 1-2 days after pollination (Lepiniec et al, 2006). Differently from flavonoids, glucosinolates (GSLs) are synthetized in maternal tissues outside of the seeds and then transported to seeds in A. thaliana (Nour-Eldin and Halkier, 2009;Sanden et al, 2023;Xu et al, 2023). 4 acid to form sinapoyl-glucose (sinapoyl-G), that constitutes the source of sinapate for the production of other sinapate-esters.…”

Section: Introductionmentioning

confidence: 99%

“…PAs are specifically synthetized in the seed coat, where they are accumulated at high concentrations at early Arabidopsis seed developmental stages, starting from 1-2 days after pollination (Lepiniec et al, 2006). Differently from flavonoids, glucosinolates (GSLs) are synthetized in maternal tissues outside of the seeds and then transported to seeds in A. thaliana (Nour-Eldin and Halkier, 2009; Sanden et al, 2023; Xu et al, 2023). Recent results nicely showed that, in A. thaliana seeds, GSL glucoiberin and glucoraphanin are synthetized in the funiculus and then transported to the seed embryo, where they accumulate and are subject to modifications (Sanden et al, 2023; Xu et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

“…Differently from flavonoids, glucosinolates (GSLs) are synthetized in maternal tissues outside of the seeds and then transported to seeds in A. thaliana (Nour-Eldin and Halkier, 2009; Sanden et al, 2023; Xu et al, 2023). Recent results nicely showed that, in A. thaliana seeds, GSL glucoiberin and glucoraphanin are synthetized in the funiculus and then transported to the seed embryo, where they accumulate and are subject to modifications (Sanden et al, 2023; Xu et al, 2023). In particular, the side chain of those two methionine-derived aliphatic GSL is hydroxylated by the 2-oxoglutarate-dependent dioxygenase AOP3 to form hydroxylated/hydroxylalkyl GSL (OH-GSL) (Kliebenstein et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Multi-omic analyses unveil temporal and spatial distribution of specialized metabolites in seeds ofCamelina sativa

Barreda,

Brosse,

Boutet

et al. 2024

Preprint

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Seeds of Brassicaceae species produce a large diversity of specialized metabolites (SMs) that strongly influence their quality, with beneficial or toxic effects on human and animal nutrition, and provide resistance to biotic or abiotic stresses. While the distribution of these compounds has been described in leaves and roots tissues, very limited information is available about their spatio-temporal accumulation in seeds of model or crop plants.Camelina sativa(camelina) is an oilseed Brassicaceae cultivated for human and animal nutrition, and for industrial uses. While we previously explored in detail SM diversity and plasticity, no information is available about SM distribution and expression of SM-related proteins and genes in camelina seeds. In this study we used untargeted metabolomics (LC-MS/MS), proteomics (DIA) and transcriptomics (RNA-Seq) to analyse synthesis, transport, modifications and degradations of SMs that are accumulated in the different seed tissues (i.e. seed coat, endosperm, and embryo) at 6 developmental and 2 germination stages. Our results showed specific patterns for many SMs, and related proteins or genes, during seed coat and embryo development. We also showed that, differently fromArabidopsis thalianaseeds, the defence and antinutritional glucosinolates compounds were accumulated in both the seed coat and endosperm, and the corresponding degradation products isothiocyanates were present at high level in the embryos of dry seeds, inC. sativa.Characterizing the spatial dynamics of seed SMs will contribute to the development of crops with an optimized distribution of beneficial and toxic metabolites for seeds quality and animal nutrition.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

“…PAs are specifically synthetized in the seed coat, where they are accumulated at high concentrations at early Arabidopsis seed developmental stages, starting from 1-2 days after pollination (Lepiniec et al, 2006). Differently from flavonoids, glucosinolates (GSLs) are synthetized in maternal tissues outside of the seeds and then transported to seeds in A. thaliana (Nour-Eldin and Halkier, 2009; Sanden et al, 2023; Xu et al, 2023). Recent results nicely showed that, in A. thaliana seeds, GSL glucoiberin and glucoraphanin are synthetized in the funiculus and then transported to the seed embryo, where they accumulate and are subject to modifications (Sanden et al, 2023; Xu et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multi-omic analyses unveil temporal and spatial distribution of specialized metabolites in seeds ofCamelina sativa

Barreda,

Brosse,

Boutet

et al. 2024

Preprint

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“…Recent studies have highlighted the role of membrane transporters in plant growth and development, as well as their adaptive pathways under abiotic stress [1,21,[190][191][192][193][194][195]. These studies have greatly improved our understanding of membrane transporters [196][197][198][199][200][201][202][203][204][205][206]. There are various types of membrane transporters.…”

Section: Discussionmentioning

confidence: 99%

Types of Membrane Transporters and the Mechanisms of Interaction between Them and Reactive Oxygen Species in Plants

Yuan,

Wu,

Jiang

et al. 2024

Antioxidants

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Membrane transporters are proteins that mediate the entry and exit of substances through the plasma membrane and organellar membranes and are capable of recognizing and binding to specific substances, thereby facilitating substance transport. Membrane transporters are divided into different types, e.g., ion transporters, sugar transporters, amino acid transporters, and aquaporins, based on the substances they transport. These membrane transporters inhibit reactive oxygen species (ROS) generation through ion regulation, sugar and amino acid transport, hormone induction, and other mechanisms. They can also promote enzymatic and nonenzymatic reactions in plants, activate antioxidant enzyme activity, and promote ROS scavenging. Moreover, membrane transporters can transport plant growth regulators, solute proteins, redox potential regulators, and other substances involved in ROS metabolism through corresponding metabolic pathways, ultimately achieving ROS homeostasis in plants. In turn, ROS, as signaling molecules, can affect the activity of membrane transporters under abiotic stress through collaboration with ions and involvement in hormone metabolic pathways. The research described in this review provides a theoretical basis for improving plant stress resistance, promoting plant growth and development, and breeding high-quality plant varieties.

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Sleeping but not defenceless: seed dormancy and protection

Hubert,

Leprince,

Buitink

2024

Journal of Experimental Botany

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To ensure their vital role in disseminating the species, dormant seeds have developed adaptive strategies to protect themselves against pathogens and predators. This is orchestrated through the synthesis of an array of constitutive defenses that are put in place in a developmentally regulated manner, which are the focus of this review. We summarize the defense activity and the nature of the molecules coming from the exudate of imbibing seeds that leak into its vicinity, also referred to as the spermosphere. As a second layer of protection, the dual role of the seed coat will be discussed; as a physical barrier and a multi-layered reservoir of defense compounds that are synthesized during seed development. Since imbibed dormant seeds can persist in the soil for extended times, we address the question if during this period, a constitutively regulated defense program is switched on to provide further protection, using the well-defined pathogenesis-related (PR) protein family. In addition, we review the hormonal and signaling pathways that might be involved in the interplay between dormancy and defense and point out questions that need further attention.

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An UMAMIT-GTR transporter cascade controls glucosinolate seed loading in Arabidopsis

Cited by 5 publications

References 32 publications

Multi-omic analyses unveil temporal and spatial distribution of specialized metabolites in seeds ofCamelina sativa

Multi-omic analyses unveil temporal and spatial distribution of specialized metabolites in seeds ofCamelina sativa

Types of Membrane Transporters and the Mechanisms of Interaction between Them and Reactive Oxygen Species in Plants

Sleeping but not defenceless: seed dormancy and protection

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