An ultrasensitive sandwich-type electrochemical immunosensor based on the signal amplification strategy of mesoporous core–shell Pd@Pt nanoparticles/amino group functionalized graphene nanocomposite

Li, Mingdang; Wang, Ping; Li, Faying; Chu, Qingyan; Li, Yueyun; Dong, Yunhui

doi:10.1016/j.bios.2016.08.076

Cited by 119 publications

(36 citation statements)

References 37 publications

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“…Cyclic voltammetry (CV) is considered to be ac onvenient and efficient method for detectingt he interfacial properties and stabilityo fm odified electrodes during their manufacture. [30,31] As shown in Figure 3A,i ft he surface of the bare GCE was modified with AuNC-PEI-rGO, the redoxpeak current was clearly higher than that of bare GCE because of the excellent conductivity of AuNC-PEI-rGO. However, the redox peak current decreased slightly if anti-NPN Ab wasl oaded onto the surface of the AuNC-PEI-rGO electrode (Ab-AuNC-PEI-rGO), because the bioactive substance greatlys uppressed the efficiency of electron transfer.…”

Section: Characterization Of the Fabricated Electrochemical Immunosensormentioning

confidence: 99%

An Ultrasensitive Electrochemical Immunosensor for Nonylphenol Leachate from Instant Noodle Containers in Southeast Asia

Hao

et al. 2019

Chemistry A European J

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Nonylphenols (NPNs) are persistent endocrine disruptors and their release into the environment is causing increasing concern about their impact on human health. Herein, an ultrasensitive electrochemical immunosensor was developed for the detection of NPNs in the leachates from 61 instant noodle containers (INCs) from 8 countries across Southeast Asia. Gold nanoclusters (AuNCs) were self‐assembled with reduced graphene oxide (rGO; polyethylenimine–rGO) and used to modify a glassy carbon electrode (GCE), which showed excellent electrical conductivity. An anti‐NPN antibody was then immobilized on the AuNCs and, if it specifically bound NPN, the reduction in conductivity of the GCE was remarkable. The designed immunosensor has a low detection limit (5.25 ng L−1) and high sensitivity for NPNs in the leachates of INCs. Remarkably, the leaching of estrogen‐like compounds from different plastics of INCs and the correlation between NPN content and total estrogenic activity were thoroughly investigated. High temperatures caused polyethylene and polystyrene INCs to release more estrogen‐like compounds than that of polypropylene INCs; this increased release of NPNs was associated with higher estrogen activity in living cells. These data fill the gap in human and environmental exposure to estrogen‐like compounds through INCs.

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Section: Characterization Of the Fabricated Electrochemical Immunosensormentioning

confidence: 99%

An Ultrasensitive Electrochemical Immunosensor for Nonylphenol Leachate from Instant Noodle Containers in Southeast Asia

Hao

et al. 2019

Chemistry A European J

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“…In general, the concentration of PSA in the serum of healthy adult males is less than 4 ng mL −1 . When the concentration of PSA is above the cutoff value of 4 ng mL −1 it implies that there is a risk of cancer, and the cancer risk significantly increases to 67 % if the PSA level is higher than 10 ng mL −1 . Therefore, sensitivity at the low‐ and sub‐ ng mL −1 range that covers the range of concern is greatly necessary.…”

Section: Introductionmentioning

confidence: 99%

“…Nanotechnology has opened up a new horizon for the diagnosis and treatment of cancers, and suggesting a dramatic future as well . With the rapid development of nanotechnology, a wide variety of nanomaterials including metal nanoparticles , metal oxides , carbon‐based nanomaterials , and nanostructured conducting polymers as well as their nanocomposites have been extensively used to fabricate electrochemical PSA immunosensors.…”

Section: Introductionmentioning

confidence: 99%

A Prostate Specific Antigen Immunosensor Based on Biotinylated‐Antibody/Cyclodextrin Inclusion Complex: Fabrication and Electrochemical Studies

et al. 2017

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Immobilization of biomolecules with a proper orientation is considered as a basis for diverse biotechnological applications. Herein, we report a host‐guest inclusion complexation between β‐cyclodextrin (β‐CD) and biotin as a versatile approach for the immobilization of biomolecules. As a practical application, a sandwich‐type electrochemical immunosensor was designed for the determination of prostate specific antigen (PSA). The immunosensor was fabricated by in situ electropolymerization of poly(N‐acetylaniline) onto a rGO‐modified Pt electrode. Then, β‐CD was covalently grafted onto the over‐oxidized polymer backbone. For improving the efficiency of the assay, AuNPs were casted on the polymeric film, on the surface of which thionine (TH) as an electron mediator was covalently immobilized. Using a host‐guest inclusion complexation between β‐CD and biotin, a β‐CD/biotin‐Ab1/PSA/Ab2‐horseradish peroxidase (HRP) sandwich was formed on the electrode surface. The analytical signal was produced via electrochemical reduction of THox, generated by biocatalytic oxidation of the THred in the presence of HRP/H2O2. Under optimal conditions, the proposed sensor responded linearly to PSA in the range from 10.0 pg mL−1 to 25.0 ng mL−1, with a low detection limit of 6.7 pg mL−1 (S/N=3). Kinetic parameters of the interaction of β‐CD with Ab1 were also investigated. Finally, the applicability of the immunosensor was successfully investigated for the detection of PSA in human serum samples.

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“…Very recently, mesoporous core-shell Pd@Pt NPs/amino group functionalized graphene nanocomposites were used for development of an ultrasensitive sandwich-type electrochemical immunosensor for PSA. The method implied a dual signal amplification strategy provided (a) by sulfo group-functionalized MWCNTs used as substrate material to enhance GCE conductivity and AuNPs enhancing the loading of primary antibodies; and (b) the nanocarriers for immobilization of Ab 2 prepared with amino group-functionalized graphene and Pd@Pt NPs providing high specific surface area, biocompatibility, and electrocatalytic activity towards the reduction of H 2 O 2 [59]. Various multiplexed configurations for the simultaneous detection of cancer biomarkers using graphene -based nanocarriers for signal amplification have also been developed.…”

Section: Graphene Used As Carrier For Signal Elements In Electrochemimentioning

confidence: 99%

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Yáñez‐Sedeño

Campuzano

Pingarrón

2017

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Growing demand for developing ultrasensitive electrochemical bioassays has led to the design of numerous signal amplification strategies. In this context, carbon-based nanomaterials have been demonstrated to be excellent tags for greatly amplifying the transduction of recognition events and simplifying the protocols used in electrochemical biosensing. This relevant role is due to the carbon-nanomaterials' large surface area, excellent biological compatibility and ease functionalization and, in some cases, intrinsic electrochemistry. These carbon-based nanomaterials involve well-known carbon nanotubes (CNTs) and graphene as well as the more recent use of other carbon nanoforms. This paper briefly discusses the advantages of using carbon nanostructures and their hybrid nanocomposites for amplification through tagging in electrochemical biosensing platforms and provides an updated overview of some selected examples making use of labels involving carbon nanomaterials, acting both as carriers for signal elements and as electrochemical tracers, applied to the electrochemical biosensing of relevant (bio)markers.

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An ultrasensitive sandwich-type electrochemical immunosensor based on the signal amplification strategy of mesoporous core–shell Pd@Pt nanoparticles/amino group functionalized graphene nanocomposite

Cited by 119 publications

References 37 publications

An Ultrasensitive Electrochemical Immunosensor for Nonylphenol Leachate from Instant Noodle Containers in Southeast Asia

An Ultrasensitive Electrochemical Immunosensor for Nonylphenol Leachate from Instant Noodle Containers in Southeast Asia

A Prostate Specific Antigen Immunosensor Based on Biotinylated‐Antibody/Cyclodextrin Inclusion Complex: Fabrication and Electrochemical Studies

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

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