An ultrasensitive label-free assay of 8-hydroxy-2′-deoxyguanosine based on the conformational switching of aptamer

Wang, Jiacheng; Wang, Yongsheng; Xue, Jiaying; Zhou, Bin; Qian, Qiu-Mei; Wang, Yong-Song; Yin, Ji-Cheng; Zhao, Hui; Liu, Hui; Liu, Shan-Du

doi:10.1016/j.bios.2014.02.047

Cited by 28 publications

(15 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mechanisms of the 8-oxo-dG-aptamer complex formation have been recently reported. 39 The 8-oxo-dG can form a complex with the 66-nt aptamer, and the structural change as a result of a G-quadruplex structure was confirmed by circular dichroism. In contrast, the G-quadruplex structure is not found during the binding between the 38-nt aptamer and 8-oxo-dG.…”

Section: Results and Discussionmentioning

confidence: 93%

“… 41 Our 38-nt aptasensor was compared with previously reported methods using the 66-nt aptamer, as shown in Table 3 . Our method surpassed Rayleigh light scattering (RLS) 39 and chiroplasmic 42 techniques in a wide linear range. Electrochemical methods 30 can provide a wider detection range than our portable device but a longer detection time.…”

Section: Results and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development of Low-Cost AuNP-Based Aptasensors with Truncated Aptamer for Highly Sensitive Detection of 8-Oxo-dG in Urine

et al. 2020

View full text Add to dashboard Cite

8-Oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dG), an oxidized form of guanosine residues, is a critical biomarker for various cancers. Herein, a sensitive citrate-capped gold nanoparticle-based aptasensor device has been developed for the detection of 8-oxo-dG in urine. We previously designed a 38-nt anti-8-oxo-dG-aptamer by a computer simulation and the experimental validation has been performed in the present work. The analytical performance of the 38-nt aptamer from the in silico design was compared with the parent 66-nt aptamer. This assay is based on the principle of salt-induced aggregation of citrate-capped gold nanoparticles. Based on this sensing mechanism, the difference between the absorbance in the presence and absence of 8-oxo-dG at λ = 525 nm (ΔA525) increased linearly as a function of 8-oxo-dG concentrations in the ranges of 10–100 and 15–100 nM for 38-nt and 66-nt aptasensors, respectively. This method can provide detection limits of 6.4 nM for 8-oxo-dG in the 38-nt aptasensor and 13.2 nM in the 66-nt aptasensor. Similar to the 66-nt aptamer, the shortened aptamer, 38-nt long, can provide high sensitivity and selectivity with rapid detection time. In addition, using the 38-nt aptamer as a recognition component in the developed portable low-cost device showed high sensitivity in the detection range of 15–100 nM with a detection limit of 12.9 nM, which is much lower than the threshold value (280 nM) for normal human urine. This easy-to-use device could effectively and economically be utilized for monitoring 8-oxo-dG in real urine samples and potentially serve as a prototype for a commercial device.

show abstract

Section: Results and Discussionmentioning

confidence: 93%

Section: Results and Discussionmentioning

confidence: 99%

Development of Low-Cost AuNP-Based Aptasensors with Truncated Aptamer for Highly Sensitive Detection of 8-Oxo-dG in Urine

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Thus, the demand for OS biomarker assays carried out in wide screening programs in point-of-care is critical. Consequently, 8-OHdG biomolecule has been quantified in various biological samples, such as, urine (Lee et al, 2010) (Subash et al, 2010)(J. C. Wang et al, 2014), saliva (Guan et al, 2014), blood (Bolner et al, 2011) and tissue (Arnett et al, 2005). It was found that the average levels of 8-OHdG in healthy humans is around 20 ng/ml, a value that increases when OS rises (Richard G. Cutler, 2002).…”

Section: Introductionmentioning

confidence: 96%

8-hydroxy-2′-deoxyguanosine (8-OHdG) biomarker detection down to picoMolar level on a plastic antibody film

Martins

Marques

Fortunato

et al. 2016

Biosensors and Bioelectronics

View full text Add to dashboard Cite

“…Under the action of the target, the DNA aptamers will produce conformational changing (Ellington & Szostak, 1990;Tuerk & Gold, 1990). Compared to the antibody, aptamers have some advantages such as thermal stability, reusability, and easy chemical synthesis, they have been widely used to recognise a wide range of substances from ions to cells in the analytical field (McKeague et al, 2014;Long, Zhu, & Wang, 2014;Wang et al, 2014;Wang et al, 2015;Xie et al, 2014;Zhu et al, 2015). Therefore, anti BPA aptamers were utilised in our developed method.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive detection of Bisphenol A based on an aptasensor with DNA amplification

Zhu

Jiang

et al. 2018

Food and Agricultural Immunology

View full text Add to dashboard Cite

An ultrasensitive and selective method for Bisphenol A (BPA) detection using aptamer-BPA and real-time quantitative polymerase chain reaction (RT-qPCR) technology is reported, in which biotin-modified aptamer DNA (BPA) is fixed on the inner wall of a streptavidin-coated PCR tube via biotin-avidin interactions and the template is fixed through the complementary base pairing with aptamer DNA. Upon the addition of BPA, the template DNA could be off from the inner wall of PCR tube, which results from aptamer DNA interacted specifically with BPA. Thus, the number of template DNA change result from the BPA concentration is readily seen by cycle threshold (Ct) values. The sensor exhibited an excellent linear response between Ct values and BPA concentration in the range of 1-500 nM and the detection limit of this method for aqueous BPA was as low as 0.7 nM. In addition, this method showed good selectivity for BPA over its analogues.

show abstract

An ultrasensitive label-free assay of 8-hydroxy-2′-deoxyguanosine based on the conformational switching of aptamer

Cited by 28 publications

References 29 publications

Development of Low-Cost AuNP-Based Aptasensors with Truncated Aptamer for Highly Sensitive Detection of 8-Oxo-dG in Urine

Development of Low-Cost AuNP-Based Aptasensors with Truncated Aptamer for Highly Sensitive Detection of 8-Oxo-dG in Urine

8-hydroxy-2′-deoxyguanosine (8-OHdG) biomarker detection down to picoMolar level on a plastic antibody film

Ultrasensitive detection of Bisphenol A based on an aptasensor with DNA amplification

Contact Info

Product

Resources

About