An ultrasensitive fluorescent biosensor using high gradient magnetic separation and quantum dots for fast detection of foodborne pathogenic bacteria

Li, Xue; Zheng, Lingyan; Zhang, Huilin; Jin, Xiu; Lin, Jianhan

doi:10.1016/j.snb.2018.03.014

Cited by 107 publications

(55 citation statements)

References 31 publications

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“…Consecuently, to include sample preconcentration steps prior to analysis is necessary [23]. Different preconcentration methods based on immunomagnetic separation (IMS) systems [24][25][26][27], centrifugation [28,29] and membrane filtration have been coupled to sensing methods. Nevertheless, membrane filtration is considered the first choice for large sample volumes (≥10 mL) [30,31].…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

et al. 2020

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Legionella is a pathogenic bacterium, ubiquitous in freshwater environments and able to colonise man-made water systems from which it can be transmitted to humans during outbreaks. The prevention of such outbreaks requires a fast, low cost, automated and often portable detection system. In this work, we present a combination of sample concentration, immunoassay detection, and measurement by chronoamperometry. A nitrocellulose microfiltration membrane is used as support for both the water sample concentration and the Legionella immunodetection. The horseradish peroxidase enzymatic label of the antibodies permits using the redox substrate 3,3′,5,5′-Tetramethylbenzidine to generate current changes proportional to the bacterial concentration present in drinking water. Carbon screen-printed electrodes are employed in the chronoamperometric measurements. Our system reduces the detection time: from the 10 days required by the conventional culture-based methods, to 2–3 h, which could be crucial to avoid outbreaks. Additionally, the system shows a linear response (R2 value of 0.99), being able to detect a range of Legionella concentrations between 101 and 104 cfu·mL−1 with a detection limit (LoD) of 4 cfu·mL−1.

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Section: Introductionmentioning

confidence: 99%

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

et al. 2020

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“…The coaxial capillary is the key to separation of target bacteria from sample background. In this study, the capillary was fabricated with some improvements based on our previous designs ( Wang et al., 2017b ; Huang et al., 2018 , Xue et al., 2018 ). Briefly, it consisted of an inner quartz capillary (inner diameter: 1.0 mm, outer diameter: 1.2 mm) and an outer quartz one (inner diameter: 1.8 mm, outer diameter: 2.0 mm).…”

Section: Methodsmentioning

confidence: 99%

Combining impedance biosensor with immunomagnetic separation for rapid screening of Salmonella in poultry supply chains

Wang

Guo

et al. 2020

Poultry Science

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Salmonella screening is a key to ensure food safety in poultry supply chains. Currently available Salmonella detection methods including culture, polymerase chain reaction and enzyme-linked immuno-sorbent assay could not achieve rapid, sensitive, and in-field detection. In this study, different strategies for separation and detection of Salmonella were proposed, compared, and improved based on our previous studies on immunomagnetic separation and impedance biosensor. First, the coaxial capillary for immunomagnetic separation of target bacteria was improved with less contamination, and 3 strategies based on the improved capillary and immunomagnetic nanoparticles were compared to separate the target bacteria from sample and form the magnetic bacteria. The experimental results showed that the strategy of capture in tube and separation in capillary was the most suitable with separation efficiency of approximately 88%. Then, the immune gold nanoparticles coated with urease were used to label the magnetic bacteria, resulting in the formation of enzymatic bacteria, which were injected into the capillary. After the urea was catalyzed by the urease on the enzymatic bacteria in the capillary, different electrodes were compared to measure the impedance of the catalysate and the screen-printed electrode with higher sensitivity and better stability was the most suitable. This impedance biosensor-based bacterial detection strategy was able to detect Salmonella as low as 10 2 CFU/mL in 2 h without complex operations. Compared to the gold standard culture method for practical screening of Salmonella in poultry supply chains, this proposed strategy had an accuracy of approximately 90% for 75 real poultry samples.

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“…IMS in combination with a fluorescent biosensor based on immune QDs was used for testing E. coli O157:H7, and the detection limit of this proposed biosensor was 14 CFU/mL. Compared with the conventional detection, the detection sensitivity was effectively improved by IMS (Xue et al., 2018). Besides, some portable fluorescent nanobiosensors were designed for screening bacterial pathogens effectively.…”

Section: The Application Of Ims In Food Microorganisms Detectionmentioning

confidence: 99%

“…In recent years, a growing number of studies have focused on enhancing the detection sensitivity and reducing the testing time from days to hours. The representative methods include immunology‐based methods (enzyme linked immunosorbent assay, ELISA; immunochromatographic assay, ICA), nucleic acid‐based methods (polymerase chain reaction, PCR; loop‐mediated isothermal amplification, LAMP), signal‐based methods (such as fluorochrome and quantum dots [QDs]), and so on (Bi et al., 2020; Li et al., 2017; Li et al., 2019; Park, Park, Ok, Chang, & Lim, 2020; Wei et al., 2019; Xue, Zheng, Zhang, Jin, & Lin, 2018). However, food samples are usually complex and heterogeneous systems.…”

Section: Introductionmentioning

confidence: 99%

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

Wang

Cai

Gao

et al. 2020

Comp Rev Food Sci Food Safe

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The high efficiency and accurate detection of foodborne pathogens and spoilage microorganisms in food are a task of great social, economic, and public health importance. However, the contamination levels of target bacteria in food samples are very low. Owing to the background interference of food ingredients and negative impact of nontarget flora, the establishment of efficient pretreatment techniques is very crucial for the detection of food microorganisms. With the sig

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An ultrasensitive fluorescent biosensor using high gradient magnetic separation and quantum dots for fast detection of foodborne pathogenic bacteria

Cited by 107 publications

References 31 publications

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

Combining impedance biosensor with immunomagnetic separation for rapid screening of Salmonella in poultry supply chains

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

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