An ultrasensitive electrochemiluminescence immunosensor based on zeolitic imidazolate frameworks encapsulating spherical graphite crystals

Zang, Ruhua; He, Yan; Yuan, Ruo

doi:10.1016/j.jelechem.2016.07.038

Cited by 14 publications

(4 citation statements)

References 24 publications

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“…Much of the work associated with sensor platforms focuses on synthesising the nanomaterials and effectively generating a stable surface for Ab immobilisation. Zang et al have aimed to improve ECL luminophore efficiency through the incorporation of zeolitic imidazolate framework nanocrystals, a subclass of metal organic frameworks (MOFs) [105]. MOFs are currently a hot topic in research due to their unique microporous structure, high surface area and tuneable chemical and physical properties, leading to extensive research in energy and gas storage, environmental applications and sensor development [106,107,108,109].…”

Section: Incorporation Of Nanomaterialsmentioning

confidence: 99%

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Point-of-Care Compatibility of Ultra-Sensitive Detection Techniques for the Cardiac Biomarker Troponin I—Challenges and Potential Value

2018

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Cardiac biomarkers are frequently measured to provide guidance on the well-being of a patient in relation to cardiac health with many assays having been developed and widely utilised in clinical assessment. Effectively treating and managing cardiovascular disease (CVD) relies on swiftly responding to signs of cardiac symptoms, thus providing a basis for enhanced patient management and an overall better health outcome. Ultra-sensitive cardiac biomarker detection techniques play a pivotal role in improving the diagnostic capacity of an assay and thus enabling a better-informed decision. However, currently, the typical approach taken within healthcare depends on centralised laboratories performing analysis of cardiac biomarkers, thus restricting the roll-out of rapid diagnostics. Point-of-care testing (POCT) involves conducting the diagnostic test in the presence of the patient, with a short turnaround time, requiring small sample volumes without compromising the sensitivity of the assay. This technology is ideal for combatting CVD, thus the formulation of ultra-sensitive assays and the design of biosensors will be critically evaluated, focusing on the feasibility of these techniques for point-of-care (POC) integration. Moreover, there are several key factors, which in combination, contribute to the development of ultra-sensitive techniques, namely the incorporation of nanomaterials for sensitivity enhancement and manipulation of labelling methods. This review will explore the latest developments in cardiac biomarker detection, primarily focusing on the detection of cardiac troponin I (cTnI). Highly sensitive detection of cTnI is of paramount importance regarding the rapid rule-in/rule-out of acute myocardial infarction (AMI). Thus the challenges encountered during cTnI measurements are outlined in detail to assist in demonstrating the drawbacks of current commercial assays and the obstructions to standardisation. Furthermore, the added benefits of introducing multi-biomarker panels are reviewed, several key biomarkers are evaluated and the analytical benefits provided by multimarkers-based methods are highlighted.

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Section: Incorporation Of Nanomaterialsmentioning

confidence: 99%

“…Figure 3 and Figure 4: [37,38,48,49,50,57,58,61,65,66,68,69,70,71,74,84,85,86,87,88,89,93,94,95,96,97,104,105,111,112,115,116,117,120,121,122,123,125,126,127,128,130,132,133,134,136,138,144,145,148,150,151,159,161,174,175,176,184,185,186,187,188,189,190,…”

Section: Reprinting Figuresmentioning

confidence: 99%

Point-of-Care Compatibility of Ultra-Sensitive Detection Techniques for the Cardiac Biomarker Troponin I—Challenges and Potential Value

2018

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“…To date, many methods have been introduced for the detection of cTnI, such as fluorescence, chemiluminescence, photoelectrochemical, enzyme-linked immunosorbent, and electrochemical assays. , The electrochemiluminescence (ECL) biosensor is also used for cTnI analysis owing to its advantages of simple equipment, high sensitivity, and short response time. − The classical ECL luminophores are luminol and Ru(bpy) 3 2+ , which have been extensively investigated . In recent decades, emerging nanomaterials such as semiconductor quantum dots, carbon materials, , and metal nanoclusters have also been widely used in the ECL system as luminescent materials.…”

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confidence: 99%

“…41 These results suggest that the HCR-based ECL system is a promising and powerful method for sensitive detection in biological analysis. For the sensitive detection of cTnI, most of the published papers are based on nanomaterials or enzymes to achieve signal amplification; [13][14][15]17 however, the detection of cTnI based on DNA amplification is rarely reported. Based on above consideration, a novel and ultrasensitive HCR-based ECL immunoassay strategy was developed for the detection of cTnI.…”

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confidence: 99%

Electrochemiluminescence Immunosensor Based on Au Nanocluster and Hybridization Chain Reaction Signal Amplification for Ultrasensitive Detection of Cardiac Troponin I

Zhu

Yan

et al. 2019

ACS Sens.

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Measurement of cardiac troponin I in the blood is crucial for the early diagnosis of acute myocardial infarction. Herein, a novel and ultrasensitive electrochemiluminescence (ECL) immunosensor has been developed for determination of cardiac troponin I (cTnI) by using Au nanoclusters and hybridization chain reaction (HCR) signal amplification. In this ECL immunosensor, Au nanoclusters were dual-labeled at each end of hairpin DNA (H 1 and H 2 ) and acted as the luminophore. DNA initiator strands (T 1 ) and secondary antibody (Ab 2 ) were conjugated on Au nanoparticles (AuNPs) to obtain a smart probe

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Electrochemical detection of the cardiac biomarker cardiac troponin I

Qin,

Li,

Qin

et al. 2024

VIEW

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Acute myocardial infarction (AMI) is a major cause of cardiovascular disease‐related death. It is essential for patients with cardiovascular disease to receive an early diagnosis of AMI. The most popular technique for the early detection of AMI is the use of biosensors to monitor the concentration of pertinent biomarkers, such as cardiac troponin I (cTnI), in the blood. The electrochemical detection methods hold great promise because of their simplicity, miniaturization, ease of integration, high sensitivity, and rapid response. The prime motive of this review is to present a comprehensive understanding of the pros and cons of methodologies employed for the electrochemical approaches toward the detection of cTnI. A detailed summary is provided for the immunosensors, aptamer sensors, molecular imprinting sensors, and peptide sensors based on various affinity elements. We enumerate the modified electrode materials for electrochemical sensors as well as popular detection techniques. Furthermore, this paper reviews some recent significant advances in point‐of‐care assays for rapid, accurate detection of cTnI as a smart integrated device for home monitoring. The accumulation of knowledge about these functions will lead to new insights into and concepts for the design of portable miniature sensors for cardiovascular patients at risk of AMI. It is anticipated that the interdisciplinary collaboration can bring more enlightenment to the progress of cardiac biomarkers sensor in the future.

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An ultrasensitive electrochemiluminescence immunosensor based on zeolitic imidazolate frameworks encapsulating spherical graphite crystals

Cited by 14 publications

References 24 publications

Point-of-Care Compatibility of Ultra-Sensitive Detection Techniques for the Cardiac Biomarker Troponin I—Challenges and Potential Value

Point-of-Care Compatibility of Ultra-Sensitive Detection Techniques for the Cardiac Biomarker Troponin I—Challenges and Potential Value

Electrochemiluminescence Immunosensor Based on Au Nanocluster and Hybridization Chain Reaction Signal Amplification for Ultrasensitive Detection of Cardiac Troponin I

Electrochemical detection of the cardiac biomarker cardiac troponin I

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