An ultrasensitive electrochemical impedance-based biosensor using insect odorant receptors to detect odorants

Khadka, Roshan; Aydemir, Nihan; Carraher, Colm; Hamiaux, Cyril; Colbert, Damon; Cheema, Jamal Ahmed; Malmström, Jenny; Kralicek, Andrew V.; Travaš-Sejdić, Jadranka

doi:10.1016/j.bios.2018.10.043

Cited by 65 publications

(50 citation statements)

References 36 publications

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“…Receptor-targeted control using ORs that are specific for a pest are likely associated with less off-target effects as compared to compounds targeting the conserved Orco 4 . Additionally, the widespread production of ipsenol and ipsdienol across many species of bark beetles makes both ItypOR46 and ItypOR49 suitable candidates to be used in sensitive biosensors 16 for detection of infestations of different bark beetles. Although there are technical challenges to overcome before such sensors can be used for airborne volatiles in a field situation, early detection of infestations and removal of attacked trees are crucial to limit bark beetle population growth, and hence outbreaks and economic loss.…”

Section: Discussionmentioning

confidence: 99%

“…From an applied perspective, functional characterization of ORs and determination of their binding sites are pertinent in pest insects, because receptors that are key to survival and reproduction represent potential targets for improved pest control using OR antagonists and agonists 4 . Also, with the advancement of biosensor technology towards using ORs to detect insect semiochemicals [15][16][17] , employing receptors tuned to the characteristic odors of a pest, such as bark beetle aggregation pheromones, could be useful for early detection of infestations.…”

Section: Introductionmentioning

confidence: 99%

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Putative ligand binding sites of two functionally characterized bark beetle odorant receptors

Yuvaraj

Roberts

Sonntag

et al. 2020

Preprint

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Bark beetle behavior is to a large extent mediated via olfaction. Targeting the odorant receptors (ORs) may thus provide avenues towards improved pest control during outbreaks. Such an approach requires information on the function of receptors and their interactions with ligands. Hence, we annotated 73 ORs from an antennal transcriptome of the spruce bark beetle Ips typographus and report the functional characterization of two ORs (ItypOR46 and ItypOR49), which are selective for single enantiomers of the common bark beetle pheromone compounds ipsenol and ipsdienol, respectively. We use homology modeling and molecular docking to predict their binding sites. The importance of residues Tyr84 and Thr205 in ItypOR46 in the activation by ipsenol is experimentally supported, and hydrogen bonding appears key in pheromone binding. The biological significance of the characterized ORs positions them as prime targets for pest control and use in biosensors to detect bark beetle infestations.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Putative ligand binding sites of two functionally characterized bark beetle odorant receptors

Yuvaraj

Roberts

Sonntag

et al. 2020

Preprint

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“…Functionalization of gold electrodes with liposome-embedded insect ORs (OR10a, OR22a, and OR71a) was also demonstrated in the detection of the target odorants methyl salicylate, methyl hexanoate, and 4-ethylguaiacol, respectively [49]. The electrode impedance response, as reflected by an obtained Nyquist plot, indicated high selectivity and femtomolar sensitivity.…”

Section: Chemo-electronic Signal Transductionmentioning

confidence: 94%

“…hOR, human odorant receptor; DmelOR, Drosophila melanogaster odorant receptor; MDL, minimum detection limit. Data cited from: Jin et al [35]; Goldsmith et al [36]; Yang et al [46]; Lee et al [47]; Murugathas et al [48]; Khadka et al [49]; Sung et al [50]; Lim et al [51]; Oh et al [52]; Park et al [53]; and Ahn et al [54].…”

Section: Or-based Bioelectronic Nosementioning

confidence: 99%

The Emergence of Insect Odorant Receptor-Based Biosensors

Bohbot

Vernick

2020

Biosensors

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The olfactory receptor neurons of insects and vertebrates are gated by odorant receptor (OR) proteins of which several members have been shown to exhibit remarkable sensitivity and selectivity towards volatile organic compounds of significant importance in the fields of medicine, agriculture and public health. Insect ORs offer intrinsic amplification where a single binding event is transduced into a measurable ionic current. Consequently, insect ORs have great potential as biorecognition elements in many sensor configurations. However, integrating these sensing components onto electronic transducers for the development of biosensors has been marginal due to several drawbacks, including their lipophilic nature, signal transduction mechanism and the limited number of known cognate receptor-ligand pairs. We review the current state of research in this emerging field and highlight the use of a group of indole-sensitive ORs (indolORs) from unexpected sources for the development of biosensors.

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“…The recent studies using AFM, XPS and Quartz crystal microbalance with dissipation monitoring (QCM-D) on three different OrXs/liposomes (Or10a, Or22a, and Or71a) revealed that the liposomes (100-200 nm) are still intact when covalently attached to a gold surface with 2/3 rd surface coverage [32]. In this study, we employed the same method to immobilize a novel receptor (Or35a) in liposomes on a gold surface and the binding of E2-hexenal to the Or35a/liposomes immobilized gold surface was investigated by EIS in the presence of PBS buffer electrolyte.…”

Section: Or35a Based Biosensor For Odorant Detection Using Eis In Pbsmentioning

confidence: 99%

Investigating Electrochemical Stability and Reliability of Gold Electrode‐electrolyte Systems to Develop Bioelectronic Nose Using Insect Olfactory Receptor

et al. 2019

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This article aims to demonstrate an electrochemically stable and reliable gold electrode‐electrolyte system to develop an insect odorant receptor (Drosophila melanogaster Or35a) based bioelectronic nose. Cyclic voltammograms (CVs) and electrochemical impedance spectroscopy (EIS) of bare gold electrodes, after modification with the self‐assembled monolayer (SAM) of 6‐mercaptohexanoic acid (MHA) and after immobilization with Or35a integrated into the lipid bilayers of liposomes were conducted in the presence of four different redox probes. Potassium ferri/ferrocyanide [Fe(CN)6]3−/[Fe (CN)6]4− and hydroquinone (H2Q) redox probes revealed variable and irreversible signals at the time scale of our measurements, with atomic force microscopy (AFM) images and x‐ray photoelectron spectroscopy (XPS) results suggesting gold surface etching due to the presence of CN− ions in case of [Fe(CN)6]3−/[Fe (CN)6]4−. Although the hexaammineruthenium complex showed stable electrochemical behaviour at all stages of biosensor development, changes in CV and EIS readings after each surface modifications were insignificant. PBS buffer as a non‐Faradaic medium, was found to provide reliable systems for electrochemical probing of modified gold electrodes with Or35a/liposomes in aqueous media. Using this system, we have shown that this novel biosensor can detect its known odorant E2‐hexenal selectively compared to methyl salicylate down to femtomolar concentration.

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An ultrasensitive electrochemical impedance-based biosensor using insect odorant receptors to detect odorants

Cited by 65 publications

References 36 publications

Putative ligand binding sites of two functionally characterized bark beetle odorant receptors

Putative ligand binding sites of two functionally characterized bark beetle odorant receptors

The Emergence of Insect Odorant Receptor-Based Biosensors

Investigating Electrochemical Stability and Reliability of Gold Electrode‐electrolyte Systems to Develop Bioelectronic Nose Using Insect Olfactory Receptor

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