An ultrasensitive electrochemical aptasensor based on Pd@PCN-222 as a signal probe coupled with exonuclease III-assisted cycling amplification for the detection of ochratoxin A

The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens

“…To address the criticism of electrochemistry's inherent instability and poor reproducibility [77], Wang et al proposed a fluorescent-electrochemical dual-signal sensing strategy based on magnetic DNA-Walker and HCR coupled amplification for the detection of Clostridium perfringens (C. perfringens) [78]. The method can significantly improve accuracy and detect C. perfringens at a minimum concentration of 1 CFU/g in meat products (e.g., chicken, beef, duck, mutton, and pork) through a multi-modal signal output approach.…”

Dual-mode sensor based on the synergy of magnetic separation and functionalized probes for the ultrasensitive detection of Clostridium perfringens

“… 25 Wu et al constructed a DNA biosensor with magnetic beads to detect the special gene fragments of Bacillus thuringiensis . 26 The beads markedly improved detection efficiency. Yang et al proposed a biosensor based on RCA, used magnetic beads as carriers, and fixed capturing probes on the surfaces of magnetic beads to facilitate separation operation.…”

“…15 Although electrochemical sensors have the advantages of high sensitivity and low detection limit, their development is greatly hindered by stability and accuracy requirements. 12,16,17 And the accuracy of the sensor detection platform that relies on a single signal cannot be guaranteed, while two independent signal transduction from different mechanisms can calibrate the system error and mutually verify the dual response. Therefore, dual-mode sensors are gradually favored by researchers.…”

“Gold-plated” PCN-222(Fe) and superconductive carbon black-based sandwich-type immunosensor for detecting CYFRA21-1

“…13 Owing to the large pore size and threedimensional (3D) structure of PCN-222(Fe), it can effectively prevent the self-aggregation of porphyrin centers, [14][15][16] showing excellent peroxidase-like catalytic activity. 17 Moreover, it is an excellent electroactive material and is widely used in applications, such as catalysis, 18 sensing applications, 19 and dye adsorption. 20 However, it is rarely used to construct electrochemical sensors because the electrical conductivity of PCN-222(Fe) needs to be improved.…”