An Ultrasensitive Colorimetric Strategy for Detection of Cadmium Based on the Peroxidase-like Activity of G-Quadruplex-Cd(II) Specific Aptamer

Zhou, Bin; Chen, Ya-Ting; Yang, Xinyi; Wang, Yongsheng; Hu, Xi‐Jiang; Suo, Qing-Li

doi:10.2116/analsci.18p248

Cited by 7 publications

(2 citation statements)

References 47 publications

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“…This method avoids using labeled oligonucleotides, makes the experimental procedure simple and convenient, allows for direct quantitative sample analysis, and has an excellent dynamic range. It displays two segments with a broad linear range, allowing Cd 2+ to be detected at concentrations as low as 0.15 nM [ 71 ].…”

Section: Colorimetric Biosensorsmentioning

confidence: 99%

Recent Aptamer-Based Biosensors for Cd2+ Detection

Gao

Wang

et al. 2023

Biosensors

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Cd2+, a major environmental pollutant, is heavily toxic to human health. Many traditional techniques are high-cost and complicated; thus, developing a simple, sensitive, convenient, and cheap monitoring approach is necessary. The aptamer can be obtained from a novel method called SELEX, which is widely used as a DNA biosensor for its easy acquisition and high affinity of the target, especially for heavy metal ions detection, such as Cd2+. In recent years, highly stable Cd2+ aptamer oligonucleotides (CAOs) were observed, and electrochemical, fluorescent, and colorimetric biosensors based on aptamers have been designed to monitor Cd2+. In addition, the monitoring sensitivity of aptamer-based biosensors is improved with signal amplification mechanisms such as hybridization chain reactions and enzyme-free methods. This paper reviews approaches to building biosensors for inspecting Cd2+ by electrochemical, fluorescent, and colorimetric methods. Finally, many practical applications of sensors and their implications for humans and the environment are discussed.

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Section: Colorimetric Biosensorsmentioning

confidence: 99%

Recent Aptamer-Based Biosensors for Cd2+ Detection

Gao

Wang

et al. 2023

Biosensors

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“…For example, Tao et al [3] used gold nanoparticle modified MoS 2 nanocomposites as an enzyme mimic to enhanced peroxidase-like activity and improved the sensibility of colorimetric Cd 2+ detection. Zhou et al [10] designed a G-rich sequence for the aptamer to bind with Cd 2+ and form a G-quadruplex structure as a peroxidase-like DNAzyme to enhance the catalytic activity of heme in order to provide a fluorescent signal for cadmium detection. Zhu et al [11] decorated Carboxyfluorescein(FAM)-labeled 3 end and poly-G structure 5 end to the aptamer and its conformation changed from a random coil sequence to a stem-loop structure when it met Cd 2+ and this resulted in the fluorescence of FAM change which linked with Cd 2+ concentration.…”

Section: Introductionmentioning

confidence: 99%

A Highly Sensitive “on-off” Time-Resolved Phosphorescence Sensor Based on Aptamer Functionalized Magnetite Nanoparticles for Cadmium Detection in Food Samples

Lai

Wang

et al. 2020

Foods

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Cadmium contamination is a severe threat to food safety. Therefore, the development of sensitive and selective cadmium detection strategies is urgently required. The elimination of background autofluorescence generated from the food matrix is critical to the optical assay for cadmium detection. Herein, a time-resolved phosphorescence sensor based on an “on-off” strategy was developed for cadmium determination in food samples. The phosphorescence nanoparticles were used as a luminous material to minimize the interference of background autofluorescence. The cadmium-binding aptamer was immobilized onto the magnetic beads and combined with a black hole quencher 1 (BHQ1) with complementary DNA as the target recognition element. With the presence of cadmium, the cadmium-binding aptamer bound to cadmium specifically and resulted in the release of BHQ1. The free BHQ1 remained in the solution after magnetic separation and quenched the phosphorescence. The phosphorescence intensity was negatively related to the concentration of cadmium. Under optimal conditions, the time-resolved phosphorescence sensor showed a linear response to cadmium concentration within a range from 0.05 to 5 ng mL−1 and with a detection limit of 0.04 ng mL−1. This “on-off” time-resolved phosphorescence sensor was successfully applied for cadmium detection in spring water and clam samples, which provided a rapid and straightforward method.

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Fluorescent indicators for live-cell and in vitro detection of inorganic cadmium dynamics

Yang

Liao

et al. 2022

J Fluoresc

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Cadmium contamination is a severe threat to the environment and food safety. Thus, there is an urgent need to develop highly sensitive and selective cadmium detection tools. The engineered uorescent indicator is a powerful tool for the rapid detection of inorganic cadmium in the environment. In this study, the development of yellow uorescent indicators of cadmium chloride by inserting a uorescent protein at different positions of the high cadmium-speci c repressor and optimizing the exible linker between the connection points is reported. These indicators provide a fast, sensitive, speci c, high dynamic range, and real-time readout of cadmium ion dynamics in solution. Under optimal conditions, the uorescent indicators N0C0/N1C1 showed a linear response to cadmium concentration within the range from 10/30 to 50/100 nM and with a detection limit of 10/33 nM. Escherichia coli cells containing the indicator were used to further study the response of cadmium ion concentration in living cells. E. coli N1C1 could respond to different concentrations of cadmium ions. This study provides a rapid and straightforward method for cadmium ion detection in vitro and the potential for biological imaging.

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An Ultrasensitive Colorimetric Strategy for Detection of Cadmium Based on the Peroxidase-like Activity of G-Quadruplex-Cd(II) Specific Aptamer

Cited by 7 publications

References 47 publications

Recent Aptamer-Based Biosensors for Cd2+ Detection

Recent Aptamer-Based Biosensors for Cd2+ Detection

A Highly Sensitive “on-off” Time-Resolved Phosphorescence Sensor Based on Aptamer Functionalized Magnetite Nanoparticles for Cadmium Detection in Food Samples

Fluorescent indicators for live-cell and in vitro detection of inorganic cadmium dynamics

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