An ultrasensitive aptamer-antibody sandwich cortisol sensor for the noninvasive monitoring of stress state

Huang, Ziyu; Chen, Hao; Ye, Huarong; Chen, Zixuan; Jaffrézic‐Renault, Nicole; Guo, Zhenzhong

doi:10.1016/j.bios.2021.113451

Cited by 69 publications

(31 citation statements)

References 35 publications

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“…Huang et al. reported a sensitive technique with a very low LOD (∼0.3 fM) using an aptamer–antibody hybrid assay; however, this high sensitivity required a long assay time and prelabeling of cortisol with a gold nanoparticle (AuNP)-conjugated antibody . Sanghavi et al reported an aptasensor based on the competitive displacement of electroactive species with a low LOD (∼32 pM) and a wide dynamic (DR) range (100 pM to 32 μM); however, it is not suitable for long-term, continuous detection .…”

Section: Resultsmentioning

confidence: 99%

Cortisol Detection in Undiluted Human Serum Using a Sensitive Electrochemical Structure-Switching Aptamer over an Antifouling Nanocomposite Layer

et al. 2021

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There is a strong and growing need to monitor stress biomarkers in vivo for real-time emotional and wellness assessment. Toward this, we report a reagent-free electrochemical aptasensor with a nanocomposite antifouling layer for sensitive and continuous detection of cortisol in human serum. A thiolated, methylene blue (MB)tagged conformation-switching aptamer was immobilized over a gold nanowire (AuNW) nanocomposite to capture cortisol and generate a signal proportional to the cortisol concentration. The signal is recorded through differential pulse voltammetry (DPV) and chronoamperometry. The aptasensor exhibited a sensitive response with 0.51 and 0.68 nM detection limits in spiked buffer and undiluted serum samples, respectively. Interference from other structurally similar analogs, namely, epinephrine and cholic acid, was negligible (<10%). The developed nanocomposite-based aptasensor showed excellent stability in undiluted human serum, outperforming several other nanocomposite materials even after prolonged exposure. This work lays the foundation for new biosensor formats such as implantable and wearable sensors.

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Section: Resultsmentioning

confidence: 99%

Cortisol Detection in Undiluted Human Serum Using a Sensitive Electrochemical Structure-Switching Aptamer over an Antifouling Nanocomposite Layer

et al. 2021

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“…The sequence partially depends on the type of modification and the purification process that is tailored to the design of each sensor. Some of the cortisol aptamers used in the formation of electrochemical immunosensors are modified with thiols (-SH) at 5′ [ 42 , 44 , 45 , 46 ], and amines (-NH 2 ) and thiols at 5′ and 3′ [ 47 , 48 , 49 ], by the HPLC purification process. The immobilization process includes a cross-linker and physical adsorption through drop casting and incubation.…”

Section: Discussionmentioning

confidence: 99%

Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol

2022

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As a steroid hormone, cortisol has a close relationship with the stress response, and therefore, can be used as a biomarker for early detection of stress. An electrochemical immunosensor is one of the most widely used methods to detect cortisol, with antibodies as its bioreceptor. Apart from conventional laboratory-based methods, the trend for cortisol detection has seemed to be exploiting antibodies and aptamers. Both can provide satisfactory performance with high selectivity and sensitivity, but they still face issues with their short shelf life. Molecularly imprinted polymers (MIPs) have been widely used to detect macro- and micro-molecules by forming artificial antibodies as bioreceptors. MIPs are an alternative to natural antibodies, which despite demonstrating high selectivity and a low degree of cross-reactivity, often also show a high sensitivity to the environment, leading to their denaturation. MIPs can be prepared with convenient and relatively affordable fabrication processes. They also have high durability in ambient conditions, a long shelf life, and the ability to detect cortisol molecules at a concentration as low as 2 ag/mL. By collecting data from the past five years, this review summarizes the antibody and aptamer-based amperometric sensors as well as the latest developments exploiting MIPs rather than antibodies. Lastly, factors that can improve MIPs performance and are expected to be developed in the future are also explained.

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“…In addition to using a single biometric element to develop sensors, Huang et al (2021) presented a highly sensitive and selective electrochemical sensor with an aptamer-antibody sandwich mode A specific combination of antibodies and aptamers was utilized to identify the target cortisol. Whereafter, Yu et al (2022) suggested a novel electrochemical aptamer sensor using functionalized graphene (FG) and nitrogen-deqcarbon quantum dots (N-CQDs) integrating MIP techniques for trace analysis of cortisol in saliva samples.…”

Section: Electrochemical Sensor For the Detection Of Cortisolmentioning

confidence: 99%

Advances in electrochemical sensors based on nanomaterials for the detection of lipid hormone

Zhang

2022

Front. Bioeng. Biotechnol.

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Lipid hormone is produced by highly differentiated endocrine cells and directly secretes into the blood circulation or tissue fluid to act as information transmission. It influences the physiological functions of the human body by controlling the metabolic processes of multiple tissue cells. Monitoring the levels of lipid hormone is of great importance for maintaining human health. The electrochemical sensor is considered as an ideal tool to detect lipid hormone owing to its advantages such as quick response, convenience and low economic costs. In recent 3 years, researchers have developed various electrochemical sensors for the detection of lipid hormone to improve their sensitivity or selectivity. The use of nanomaterials (such as carbon nanomaterials, precious metal and polymer) is a key research object and a breakthrough for improving the sensing performance of electrochemical sensors for detection of lipid hormone. This paper reviews and discusses the basic principle, nanomaterials, actuality and future development trend of electrochemical sensors for the detection of lipid hormone in the past 3 years.

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An ultrasensitive aptamer-antibody sandwich cortisol sensor for the noninvasive monitoring of stress state

Cited by 69 publications

References 35 publications

Cortisol Detection in Undiluted Human Serum Using a Sensitive Electrochemical Structure-Switching Aptamer over an Antifouling Nanocomposite Layer

Cortisol Detection in Undiluted Human Serum Using a Sensitive Electrochemical Structure-Switching Aptamer over an Antifouling Nanocomposite Layer

Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol

Advances in electrochemical sensors based on nanomaterials for the detection of lipid hormone

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