An ultrasensitive and ultraselective chemiluminescence aptasensor for dopamine detection based on aptamers modified magnetic mesoporous silica @ graphite oxide polymers

Sun, Yuanling; Lin, Yanna; Ding, Chaofan; Sun, Wei; Dai, Yuxue; Zhu, Xiaodong; Líu, Hao; Luo, Chuannan

doi:10.1016/j.snb.2017.10.171

Cited by 57 publications

(14 citation statements)

References 45 publications

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“…An optimal target concentration is used to avoid nonspecific binding and to improve the efficiency of the SELEX selection procedure. Various strategies have been proposed and successfully exploited, including (i) addition of spacer molecules that can change the length of aptamers, (ii) coupling with a fluorescent molecule, and (iii) incorporation of unnatural bases that can help to increase the diversity of catalytic molecules [80][81][82]124,125]. The impact of optimal target concentration and nonspecific binding has been assessed in relation to the efficiency of the selection process [126].…”

Section: Target Concentrationmentioning

confidence: 99%

“…For CRET-based aptasensors, peroxidase-mimicking DNAzymes were conjugated to QDs [80]. The CRET principle was incorporated into many aptamers to further develop the sensing platform [81]. In addition to QDs, other nanomaterials such as graphene oxide have been employed in chemiluminescent aptasensors [82,83].…”

Section: Colorimetric Aptazyme Sensorsmentioning

confidence: 99%

“…The desirable properties of DNAzymes (e.g., low immunogenicity and modifiable chemical structure) make them appropriate candidates for targeted drug delivery relative to other methods such as liposomes and nanocapsulations. DNAzymes can act as stimulus-responsive caps to the pores of nanocarriers such as AuNPs and mesoporous SiO 2 NPs [81,110]. In the presence of an activating factor, DNAzyme-mediated cleavage can unlock nanocarrier pores to allow the controlled release of drugs.…”

Section: Dnazymes For Targeted Drug Delivery and Therapymentioning

confidence: 99%

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Advanced Selection Methodologies for DNAzymes in Sensing and Healthcare Applications

Kumar

Jain

Dilbaghi

et al. 2019

Trends in Biochemical Sciences

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DNAzymes have been widely explored owing to their excellent catalytic activity in a broad range of applications, notably in sensing and biomedical devices. These newly discovered applications have built high hopes for designing novel catalytic DNAzymes. However, the selection of efficient DNAzymes is a challenging process but one that is of crucial importance. Initially, systemic evolution of ligands by exponential enrichment (SELEX) was a labor-intensive and time-consuming process, but recent advances have accelerated the automated generation of DNAzyme molecules. This review summarizes recent advances in SELEX that improve the affinity and specificity of DNAzymes. The thriving generation of new DNAzymes is expected to open the door to several healthcare applications. Therefore, a significant portion of this review is dedicated to various biological applications of DNAzymes, such as sensing, therapeutics, and nanodevices. In addition, discussion is further extended to the barriers encountered for the real-life application of these DNAzymes to provide a foundation for future research. Glossary Chromatography: a technique used for the physical separation of mixtures into their components. All forms of chromatography work on the same principle, in that, all have a stationary phase and a mobile phase. The approach is wellknown for its high sensitivity because of competitive interaction between the desired molecule and target immobilized stationary phase that are mainly composed of agarose beads. Combinatorial chemistry: synthetic method used to prepare a library of structurally related analogues in a single process. Currently, vast ranges of simulation software are used for generating combinatorial library. Electrochemical detection: the basic principle of electrochemical biosensors involves immobilization of selected functional DNA molecules on an electrode surface that specifically bind to a ligand, which can further lead to a change in the current or voltage. Electrophoresis: analysis technique used to efficiently separate charged particles from each another based on differences in their mobility. It can be used to separate a variety of small and large target molecules. The major shortcoming associated is that the target molecule should have a sufficient size to induce mobility changes. G-quadruplex DNAzyme: single-stranded G-rich aptamer sequences spontaneously form an intramolecular four-stranded helical structure (G4). Associates with the hemin group to form the catalytic G4-hemin-DNAzyme complex. This complex DNAzyme catalyzes the oxidation reaction of hydrogen peroxide to result in the generation of chemiluminescence. Logic gate: a logic gate performs a logical operation (Boolean algebra) on given inputs in order to generate a single electrical output. Different logic gates are combined using combinatorial or sequential approaches for computer programming. Basic logic gate series include-AND, OR, NOT, XOR, NOR, NAND, and XNOR.

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Section: Target Concentrationmentioning

confidence: 99%

Section: Colorimetric Aptazyme Sensorsmentioning

confidence: 99%

Section: Dnazymes For Targeted Drug Delivery and Therapymentioning

confidence: 99%

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Advanced Selection Methodologies for DNAzymes in Sensing and Healthcare Applications

Kumar

Jain

Dilbaghi

et al. 2019

Trends in Biochemical Sciences

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“…So, it is significant to determine the concentration of DA in biological fluids. Several methods have been proposed for the analysis of DA, including fluorescence [2], electrochemistry [3,4], chemiluminescence [5], chromatography [6], and colorimetric method [7]. Among these methods, the colorimetric method has the advantages of low cost, simple operation, fast mensuration, good accuracy, and reproducibility.…”

Section: Introductionmentioning

confidence: 99%

Triple-enzyme mimetic activity of Fe3O4@C@MnO2 composites derived from metal–organic frameworks and their application to colorimetric biosensing of dopamine

et al. 2021

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Novel Fe 3 O 4 @C@MnO 2 composites were successfully synthesized for the first time via an interfacial reaction between magnetic porous carbon and KMnO 4 , in which the magnetic porous carbon was derived from the pyrolysis of Fe-MIL-88A under N 2 atmosphere. Interestingly, the obtained Fe 3 O 4 @C@MnO 2 composites were found to have triple-enzyme mimetic activity including peroxidase-like, catalase-like, and oxidase-like activity. As a peroxidase mimic, Fe 3 O 4 @C@MnO 2 composites could catalyze the oxidation of TMB into a blue oxidized product by H 2 O 2 . As a catalase mimic, Fe 3 O 4 @C@MnO 2 could catalyze the decomposition of H 2 O 2 to generate O 2 and H 2 O. As an oxidase mimic, Fe 3 O 4 @C@MnO 2 could catalyze the direct oxidation of TMB to produce a blue oxidized product without H 2 O 2 . Reactive oxygen species measurements revealed that the oxidase-like activity originated from 1 O 2 and O 2 − •and little•OH generated by the dissolved oxygen, which was catalyzed by the Fe 3 O 4 @C@MnO 2 in the TMB oxidation reaction. The oxidase-like activity of Fe 3 O 4 @C@MnO 2 was investigated in detail. Under the optimized conditions, a rapid, sensitive, visual colorimetric method for dopamine detection was developed based on the inhibitory effect of dopamine on the oxidase-like activity. The proposed method allows for dopamine detection with a limit of detection of 0.034 μM and a linear range of 0.125-10 μM. This new colorimetric method was successfully used for the determination of dopamine in human blood samples. KeywordsFe 3 O 4 @C@MnO 2 composites • Triple-enzyme mimetic activity • Oxidase mimic • Colorimetric biosensing • Dopamine * Xiaohong Hou

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“…Several analytical methods including high-performance liquid chromatography (HPLC) [ 7 , 8 ], capillary electrophoresis [ 9 ], colorimetry [ 10 , 11 , 12 ], fluorimetry [ 13 , 14 , 15 , 16 ], chemiluminescence [ 17 , 18 ] and electrochemical methods [ 19 , 20 , 21 ] have been reported for dopamine detection. These methods have many disadvantages such as time consuming, requires a complex and trained personal tool, expensive and not suitable for on-site analysis.…”

Section: Introductionmentioning

confidence: 99%

Solid-Contact Potentiometric Sensors Based on Stimulus-Responsive Imprinted Polymers for Reversible Detection of Neutral Dopamine

et al. 2020

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Herein, we present for the first time a novel potentiometric sensor based on the stimulus-responsive molecularly imprinted polymer (MIP) as a selective receptor for neutral dopamine determination. This smart receptor can change its capabilities to recognize according to external environmental stimuli. Therefore, MIP-binding sites can be regenerated in the polymeric membrane by stimulating with stimulus after each measurement. Based on this effect, reversible detection of the analyte via potentiometric transduction can be achieved. MIPs based on 4-vinylphenylboronic acid as the functional monomer were prepared as the selective receptor. This monomer can successfully bind to dopamine via covalent binding and forming a five- or six-membered cyclic ester in a weakly alkaline aqueous solution. In acidic medium, the produced ester dissociates and regenerates new binding sites in the polymeric membrane. The proposed smart sensor exhibited fast response and good sensitivity towards dopamine with a limit of detection 0.15 µM over the linear range 0.2–10 µM. The selectivity pattern of the proposed ISEs was also evaluated and revealed an enhanced selectivity towards dopamine over several phenolic compounds. Constant-current chronopotentiometry is used for evaluating the short-term potential stability of the proposed ISEs. The obtained results confirm that the stimulus-responsive MIPs provide an attractive way towards reversible MIP-based electrochemical sensors designation.

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An ultrasensitive and ultraselective chemiluminescence aptasensor for dopamine detection based on aptamers modified magnetic mesoporous silica @ graphite oxide polymers

Cited by 57 publications

References 45 publications

Advanced Selection Methodologies for DNAzymes in Sensing and Healthcare Applications

Advanced Selection Methodologies for DNAzymes in Sensing and Healthcare Applications

Triple-enzyme mimetic activity of Fe3O4@C@MnO2 composites derived from metal–organic frameworks and their application to colorimetric biosensing of dopamine

Solid-Contact Potentiometric Sensors Based on Stimulus-Responsive Imprinted Polymers for Reversible Detection of Neutral Dopamine

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