An ultra high-efficiency droplet microfluidics platform using automatically synchronized droplet pairing and merging

Zhang, Han; Guzman, Adrian R.; Wippold, Jose A; Li, Yuwen; Dai, Jing; Huang, Can; Han, Arum

doi:10.1039/d0lc00757a

Cited by 36 publications

(38 citation statements)

References 37 publications

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“…An advantage with this setup is that the polymerisation takes place not only on the surface but also inside the polymer tank. Han et al used a two-photon photolithography system (Nanoscribe 3D Photonics) to create master moulds curved structures, which is demanding, for complex liquid handling within a droplet microfluidic system [147]. Lu et al have described fabrication of a micro-mechanical device using projection µSL [148].…”

Section: D Printingmentioning

confidence: 99%

Fabrication Methods for Microfluidic Devices: An Overview

2021

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Microfluidic devices offer the potential to automate a wide variety of chemical and biological operations that are applicable for diagnostic and therapeutic operations with higher efficiency as well as higher repeatability and reproducibility. Polymer based microfluidic devices offer particular advantages including those of cost and biocompatibility. Here, we describe direct and replication approaches for manufacturing of polymer microfluidic devices. Replications approaches require fabrication of mould or master and we describe different methods of mould manufacture, including mechanical (micro-cutting; ultrasonic machining), energy-assisted methods (electrodischarge machining, micro-electrochemical machining, laser ablation, electron beam machining, focused ion beam (FIB) machining), traditional micro-electromechanical systems (MEMS) processes, as well as mould fabrication approaches for curved surfaces. The approaches for microfluidic device fabrications are described in terms of low volume production (casting, lamination, laser ablation, 3D printing) and high-volume production (hot embossing, injection moulding, and film or sheet operations).

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Section: D Printingmentioning

confidence: 99%

Fabrication Methods for Microfluidic Devices: An Overview

2021

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“…The COMSOL Multiphysics 5.5a software (COMSOL Inc.) was used for the temperature profile simulation of the viral solution. To perform this finite element analysis, a 3D geometry was chosen, where the geometry was created using the following initial and boundary conditions (Zhang et al, 2019, 2020): no‐slip conditions on the tubing surfaces; the fluid is Newtonian and the flow within the channel is incompressible; no viscous stress and convective flux on the tubing outlet; convective heat flux is considered as the source of heat influx from the oil to the tubing.…”

Section: Methodsmentioning

confidence: 99%

Sub‐second heat inactivation of coronavirus using a betacoronavirus model

Jiang

Zhang

Wippold

et al. 2021

Biotech & Bioengineering

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Heat treatment denatures viral proteins that comprise the virion, making the virus incapable of infecting a host. Coronavirus (CoV) virions contain single‐stranded RNA genomes with a lipid envelope and four proteins, three of which are associated with the lipid envelope and thus are thought to be easily denatured by heat or surfactant‐type chemicals. Prior studies have shown that a temperature as low as 75°C with a treatment duration of 15 min can effectively inactivate CoV. The degree of CoV heat inactivation greatly depends on the length of heat treatment time and the temperature applied. With the goal of finding whether sub‐second heat exposure of CoV can sufficiently inactivate CoV, we designed and developed a simple fluidic system that can measure sub‐second heat inactivation of CoV. The system is composed of a stainless‐steel capillary immersed in a temperature‐controlled oil bath followed by an ice bath, through which virus solution can flow at various speeds. Flowing virus solution at different speeds, along with temperature control and monitoring system, allows the virus to be exposed to the desired temperature and treatment durations with high accuracy. Using mouse hepatitis virus, a betacoronavirus, as a model CoV system, we identified that 71.8°C for 0.51 s exposure is sufficient to obtain >5 Log 10 reduction in viral titer (starting titer: 5 × 10 7 PFU/ml), and that when exposed to 83.4°C for 1.03 s, the virus was completely inactivated (>6 Log 10 reduction).

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“…This strategy allowed continuous aqueous-phase flow and cleavage of the waterin-oil emulsion droplets, permitting sequential manipulation. This versatile PDMS-based device enabled droplet generation, reflow, cleaving, pairing, and cocultivation [49].…”

Section: Multilayer Soft Lithography Using Pdmsmentioning

confidence: 99%

Latest Updates on the Advancement of Polymer-Based Biomicroelectromechanical Systems for Animal Cell Studies

Garcia-Ramirez

Cerda-Kipper

Alvarez

et al. 2021

Advances in Polymer Technology

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Biological sciences have reached the fundamental unit of life: the cell. Ever-growing field of Biological Microelectromechanical Systems (BioMEMSs) is providing new frontiers in both fundamental cell research and various practical applications in cell-related studies. Among various functions of BioMEMS devices, some of the most fundamental processes that can be carried out in such platforms include cell sorting, cell separation, cell isolation or trapping, cell pairing, cell-cell communication, cell differentiation, cell identification, and cell culture. In this article, we review each mentioned application in great details highlighting the latest advancements in fabrication strategy, mechanism of operation, and application of these tools. Moreover, the review article covers the shortcomings of each specific application which can open windows of opportunity for improvement of these devices.

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An ultra high-efficiency droplet microfluidics platform using automatically synchronized droplet pairing and merging

Abstract: Droplet microfluidics systems hold great promise in their ability to conduct high-throughput assays for a broad range of life science applications. Despite their capability of conducting complex liquid handling steps...

Cited by 36 publications

References 37 publications

Fabrication Methods for Microfluidic Devices: An Overview

Fabrication Methods for Microfluidic Devices: An Overview

Sub‐second heat inactivation of coronavirus using a betacoronavirus model

Latest Updates on the Advancement of Polymer-Based Biomicroelectromechanical Systems for Animal Cell Studies

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