An SPR investigation into the therapeutic drug monitoring of the anticancer drug imatinib with selective aptamers operating in human plasma

Tartaggia, Stefano; Meneghello, Anna; Bellotto, Ottavia; Poetto, Ariana Soledad; Zanchetta, Martina; Posocco, Bianca; Bunka, David H. J.; Polo, Federico; Toffoli, Giuseppe

doi:10.1039/d0an01860k

Cited by 12 publications

(7 citation statements)

References 44 publications

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“…Aptamer discovery and development was performed by Aptamer Group (York, UK) according to proprietary selection methods. − These aptamers are commercially known as Optimer binders. Briefly, the SARS-CoV-2 S1 domain of the Spike protein (Sino Biological, Eschborn, Germany) was immobilized onto nickel beads via His-tags and used for DNA aptamer selection from a library of 10 14 sequences via eight successive rounds of in vitro selection.…”

Section: Methodsmentioning

confidence: 99%

SARS-CoV-2 Aptasensors Based on Electrochemical Impedance Spectroscopy and Low-Cost Gold Electrode Substrates

Lasserre

Balansethupathy²,

Vezza

et al. 2022

Anal. Chem.

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SARS-CoV-2 diagnostic practices broadly involve either quantitative polymerase chain reaction (qPCR)-based nucleic amplification of viral sequences or antigen-based tests such as lateral flow assays (LFAs). Reverse transcriptase-qPCR can detect viral RNA and is the gold standard for sensitivity. However, the technique is time-consuming and requires expensive laboratory infrastructure and trained staff. LFAs are lower in cost and near real time, and because they are antigen-based, they have the potential to provide a more accurate indication of a disease state. However, LFAs are reported to have low real-world sensitivity and in most cases are only qualitative. Here, an antigen-based electrochemical aptamer sensor is presented, which has the potential to address some of these shortfalls. An aptamer, raised to the SARS-CoV-2 spike protein, was immobilized on a low-cost gold-coated polyester substrate adapted from the blood glucose testing industry. Clinically relevant detection levels for SARS-CoV-2 are achieved in a simple, label-free measurement format using sample incubation times as short as 15 min on nasopharyngeal swab samples. This assay can readily be optimized for mass manufacture and is compatible with a low-cost meter.

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Section: Methodsmentioning

confidence: 99%

SARS-CoV-2 Aptasensors Based on Electrochemical Impedance Spectroscopy and Low-Cost Gold Electrode Substrates

Lasserre

Balansethupathy²,

Vezza

et al. 2022

Anal. Chem.

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“…The results obtained in a fluorescence-based test in solution [37] have already shown a higher binding affinity compared to the previous peptides P 1 and P 2 , paving the way to its use in biosensing as a candidate receptor for h-uPA. In this context, label-free biosensing platforms offer highly performing, cost-affordable, user-friendly, and potentially scalable solutions for the detection of several types of cancer biomarkers [46][47][48], from proteins up to exosomes [49][50][51], and for therapeutic drug monitoring [52]. Therefore, they are particularly appealing when developing biosensing platforms enabling point-of-care (PoC) diagnostics.…”

Section: Introductionmentioning

confidence: 99%

An Impedimetric Biosensing Strategy Based on BicyclicPeptides as Bioreceptors for Monitoring h-uPA Cancer Biomarkers

et al. 2023

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In the era of liquid biopsies, the reliable and cost-effective detection and screening of cancer biomarkers has become of fundamental importance, thus paving the way for the advancement of research in the field of point-of-care testing and the development of new methodologies and technologies. Indeed, the latter ones can help designing advanced diagnostic tools that can offer portability, ease of use with affordable production and operating costs. In this respect, impedance-based biosensing platforms might represent an attractive alternative. In this work, we describe a proof-of-concept study aimed at designing portable impedimetric biosensors for the monitoring of human urokinase-type plasminogen activator (h-uPA) cancer biomarker by employing small synthetic receptors. Aberrant levels of h-uPA were correlated with different types of cancers. Herein, we report the use of two bicyclic peptides (P2 and P3) which have been engineered to bind h-uPA with high affinity and exquisite specificity. The synthetic receptors were immobilized via biotin-streptavidin chemistry on the surface of commercial screen-printed electrodes. The impedimetric changes in the electrode/solution interface upon incubation of spiked h-uPA samples in the presence of a redox probe were followed via electrochemical impedance spectroscopy. The P3-based impedimetric assay showed the best outcomes in terms of dynamic range and linearity (0.01–1 μg mL−1) and sensitivity (LOD = 9 ng mL−1). To fully assess the performances of P3 over P2, and to compare the label-free architecture vs. labelled architecture, a voltammetric assay was also developed.

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“…Small molecules are ubiquitous, taking the form of metabolites, drugs, toxins, etc. [1][2][3] , and have been explored for pain relief 4 and treatment of cardiac diseases 5 , cancer 6 , and infectious diseases 7 including COVID-19 8 . It is critical to differentiate relatively subtle differences in small molecules, because they can encipher drastic differences in the molecular bioactivities.…”

Section: Introductionmentioning

confidence: 99%

Picomolar-Level Sensing of Cannabidiol by Metal Nanoparticles Functionalized with Chemically Induced Dimerization Binders

Ikbal,

Kang,

Chen

et al. 2023

Preprint

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Simple and fast detection of small molecules is critical to health and environmental monitoring. Methods for chemical detection often use mass spectrometers or enzymes; the former relies on expensive equipment and the latter is limited to those that can act as enzyme substrates. Affinity reagents like antibodies can target a variety of small-molecule analytes, but the detection requires successful design of chemically conjugated targets or analogs for competitive binding assays. Here, we developed a generalizable method for highly sensitive and specific in-solution detection of small molecules, using cannabidiol (CBD) as an example. Our sensing platform uses gold nanoparticles (AuNPs) functionalized with a pair of chemically induced dimerization (CID) nanobody binders (nano-binders), where CID triggers AuNPs aggregation and sedimentation in the presence of CBD. Despite moderate binding affinities of the two nano-binders to CBD (KDs of ~6 and ~56 micromolar), a scheme consisting of CBD-AuNP pre-analytical incubation, centrifugation, and electronic detection (ICED) was devised to demonstrate a high sensitivity (limit of detection of ~100 picomolar) in urine and saliva, a relatively short assay time (~2 hours), a large dynamic range (5 logs), and a sufficiently high specificity to differentiate CBD from its analog, tetrahydrocannabinol. The high sensing performance was achieved with the multivalency of AuNP sensing, the ICED scheme that increases analyte concentrations in a small assay volume, and a portable electronic detector. This sensing system is readily coupled to other binders for wide molecular diagnostic applications.

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An SPR investigation into the therapeutic drug monitoring of the anticancer drug imatinib with selective aptamers operating in human plasma

Abstract: An ss-DNA aptamer-based biosensor was devised to detect the anticancer drug imatinib by means of surface plasmon resonance.

Cited by 12 publications

References 44 publications

SARS-CoV-2 Aptasensors Based on Electrochemical Impedance Spectroscopy and Low-Cost Gold Electrode Substrates

SARS-CoV-2 Aptasensors Based on Electrochemical Impedance Spectroscopy and Low-Cost Gold Electrode Substrates

An Impedimetric Biosensing Strategy Based on BicyclicPeptides as Bioreceptors for Monitoring h-uPA Cancer Biomarkers

Picomolar-Level Sensing of Cannabidiol by Metal Nanoparticles Functionalized with Chemically Induced Dimerization Binders

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