An S18 ribosomal protein gene copy at the Arabidopsis PFL locus affects plant development by its specific expression in meristems.

Lijsebettens, Mieke Van; Vanderhaeghen, Rudy; Block, Marc De; Bauw, Guy; Villarroel, Raimundo; Montagu, Marc Van

doi:10.1002/j.1460-2075.1994.tb06640.x

Cited by 185 publications

(185 citation statements)

References 51 publications

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“…Interestingly, the retarded growth, pointed leaf, and cellular disorganization phenotype observed in the Atnuc-L1 plants is reminiscent of phenotypes reported in two mutated ribosomal protein gene loci, RPS13 (Ito et al, 2000) and RPS18 (Van Lijsebettens et al, 1994), suggesting a functional relationship between AtNUC-L1 and some early steps of ribosome assembly. Indeed, we have identified RPS13 and RPS18 associated with a large U3snoRNP complex containing nucleolin-like protein (our unpublished data), which is involved in early processing of pre-rRNA in B. oleracea (Saez-Vasquez et al, 2004).…”

Section: Discussionmentioning

confidence: 63%

Characterization of AtNUC-L1 Reveals a Central Role of Nucleolin in Nucleolus Organization and Silencing of AtNUC-L2 Gene in Arabidopsis

Pontvianne

Matı́a

Douet

et al. 2007

MBoC

124

207

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Nucleolin is one of the most abundant protein in the nucleolus and is a multifunctional protein involved in different steps of ribosome biogenesis. In contrast to animals and yeast, the genome of the model plant Arabidopsis thaliana encodes two nucleolin-like proteins, AtNUC-L1 and AtNUC-L2. However, only the AtNUC-L1 gene is ubiquitously expressed in normal growth conditions. Disruption of this AtNUC-L1 gene leads to severe plant growth and development defects. AtNUC-L1 is localized in the nucleolus, mainly in the dense fibrillar component. Absence of this protein in Atnuc-L1 plants induces nucleolar disorganization, nucleolus organizer region decondensation, and affects the accumulation levels of pre-rRNA precursors. Remarkably, in Atnuc-L1 plants the AtNUC-L2 gene is activated, suggesting that AtNUC-L2 might rescue, at least partially, the loss of AtNUC-L1. This work is the first description of a higher eukaryotic organism with a disrupted nucleolin-like gene and defines a new role for nucleolin in nucleolus structure and rDNA chromatin organization.

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Section: Discussionmentioning

confidence: 63%

Characterization of AtNUC-L1 Reveals a Central Role of Nucleolin in Nucleolus Organization and Silencing of AtNUC-L2 Gene in Arabidopsis

Pontvianne

Matı́a

Douet

et al. 2007

MBoC

124

207

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“…ethz.ch). Although RPL10B transcript levels were consistently lower than those of RPL10A and RPL10C, this gene is highly regulated during development: its expression profile is similar to other characterized Arabidopsis ribosomal proteins, such as RPS5A and RPS5B (Weijers et al, 2001), RPS18A to RPS18C (Van Lijsebettens et al, 1994;Vanderhaeghen et al, 2006), RPL23aA and RPL23aB Bonham-Smith, 2008a, 2008b), and RPL11A and RPL11B (Williams and Sussex, 1995), where transcripts accumulate to the highest levels in mitogenic tissues and the lowest in nondividing tissues (Byrne, 2009). …”

Section: Expression Of Rpl10 Transcripts In Arabidopsis and Maizementioning

confidence: 85%

“…Mutations in ribosomal proteins have been reported as showing abnormalities in growth and development (Van Lijsebettens et al, 1994;Ito et al, 2000;Weijers et al, 2001;Nishimura et al, 2005;Degenhardt and Bonham-Smith, 2008a;Imai et al, 2008, Pinon et al, 2008Yao et al, 2008;Byrne, 2009;Fujikura et al, 2009;Rosado et al 2010). As mentioned above, homozygous rpl10B plants were significantly underrepresented.…”

Section: Identification and Characterization Of Rpl10 Arabidopsis Insmentioning

confidence: 99%

Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress

et al. 2010

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(J.B., A.L.B.) Ribosomal protein L10 (RPL10) proteins are ubiquitous in the plant kingdom. Arabidopsis (Arabidopsis thaliana) has three RPL10 genes encoding RPL10A to RPL10C proteins, while two genes are present in the maize (Zea mays) genome (rpl10-1 and rpl10-2). Maize and Arabidopsis RPL10s are tissue-specific and developmentally regulated, showing high levels of expression in tissues with active cell division. Coimmunoprecipitation experiments indicate that RPL10s in Arabidopsis associate with translation proteins, demonstrating that it is a component of the 80S ribosome. Previously, ultraviolet-B (UV-B) exposure was shown to increase the expression of a number of maize ribosomal protein genes, including rpl10. In this work, we demonstrate that maize rpl10 genes are induced by UV-B while Arabidopsis RPL10s are differentially regulated by this radiation: RPL10A is not UV-B regulated, RPL10B is down-regulated, while RPL10C is up-regulated by UV-B in all organs studied. Characterization of Arabidopsis T-DNA insertional mutants indicates that RPL10 genes are not functionally equivalent. rpl10A and rpl10B mutant plants show different phenotypes: knockout rpl10A mutants are lethal, rpl10A heterozygous plants are deficient in translation under UV-B conditions, and knockdown homozygous rpl10B mutants show abnormal growth. Based on the results described here, RPL10 genes are not redundant and participate in development and translation under UV-B stress.

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“…A striking demonstration that a change in expression of a ribosomal protein gene can affect development is provided by the observation that a mutation in one of the three genes encoding cytoplasmic ribosomal protein S18 (S18A) in Arabidopsis results in plants with pointed first leaves, reduced fresh weight, and retarded growth (25). The three genes are all transcribed and encode completely identical proteins; however, no transcript is detected from the mutated gene, designated S18A.…”

Section: Discussionmentioning

confidence: 99%

Subpopulations of chloroplast ribosomes change during photoregulated development of Zea mays leaves: Ribosomal proteins L2, L21, and L29

Zhao¹,

Xu²,

Zucchi³

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

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Seedlings grown in darkness, i.e., etiolated seedlings, lack chlorophyll and most other components of the photosynthetic apparatus. On illumination, the plastids become photosynthetically competent through the production of chlorophylls and proteins encoded by certain chloroplast and nuclear genes. There are two types of photosynthetic cells in leaves of the C4 plant maize: bundle sheath cells (BSC) and adjacent mesophyll cells (MC). Some proteins of the maize photosynthetic machinery are solely or preferentially localized in MC and others in BSC. A particular gene may be photoregulated up in one cell type and down in the other. Transcripts of the nuclear gene rpl29, encoding the chloroplast ribosomal protein L29, increase in abundance about 17-fold during light-induced maturation of plastids. There is about 1.5 times more L29 protein in ribosomes of greening leaves than in ribosomes of unilluminated leaves; the L29 contents of MC and BSC are about the same. However, L21 is present about equally in plastid ribosomes of unilluminated and illuminated seedlings. In contrast to both L29 and L21, the fraction of the ribosome population containing L2 is about the same in MC and BSC of etiolated leaves but, on illumination, the proportion of the ribosome population with L2 increases in BSC but not in MC. The existence of different subpopulations of plastid ribosomes-e.g., those with and without L21 and͞or L29 during development-evokes interesting, but as yet unanswered, questions about the roles of different types of ribosomes in differentiation.When germinated and grown in darkness, angiosperm seedlings are etiolated. They lack chlorophyll and many protein components of the photosynthetic apparatus. On illumination, the required components of the photosynthetic apparatus form, and the seedlings become photosynthetically competent.Maize is a C4 plant. Certain steps in photosynthetic carbon fixation are limited to the mesophyll cells (MC) of the leaf, and other photosynthetic processes, including carbon dioxide fixation by ribulose bisphosphate carboxylase, are limited to the adjacent bundle sheath cells (BSC). MC have both of the two photosynthetic energy transducing photosystems (PSI and PSII), but BSC are poor in the oxygen evolving PSII. MC and BSC are morphologically distinct in etiolated leaves. In the course of light-induced development, genes for some components of the photosynthetic apparatus are expressed in both MC and BSC, but others are expressed differently in the two types of cells.Plastid genes whose transcript levels change on illumination of dark-grown seedlings have been identified by hybridizing mRNAs prepared from unilluminated and illuminated leaf tissues against restriction fragments of plastid DNA (e.g., 1-3). Genes for the large subunit of ribulose bisphosphate carboxylase and genes for components of the energy-transducing elements of the photosynthetic apparatus are prominent among those whose expression has been shown to be promoted on illumination of dark-grown maize seedlings.To broa...

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An S18 ribosomal protein gene copy at the Arabidopsis PFL locus affects plant development by its specific expression in meristems.

Cited by 185 publications

References 51 publications

Characterization of AtNUC-L1 Reveals a Central Role of Nucleolin in Nucleolus Organization and Silencing of AtNUC-L2 Gene in Arabidopsis

Characterization of AtNUC-L1 Reveals a Central Role of Nucleolin in Nucleolus Organization and Silencing of AtNUC-L2 Gene in Arabidopsis

Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress

Subpopulations of chloroplast ribosomes change during photoregulated development of Zea mays leaves: Ribosomal proteins L2, L21, and L29

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