An S receptor kinase gene in self-compatible Brassica napus has a 1-bp deletion.

Goring, Daphne R.; Glavin, Tracy L.; Schäfer, Ulrike; Rothstein, Steven J.

doi:10.1105/tpc.5.5.531

Cited by 142 publications

(65 citation statements)

References 22 publications

(22 reference statements)

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“…However, as the scfl mutation affects the expression level of at least three S genes (SLG, SLR and SLR2), it is not possible to attribute the modification of the self-incompatibility reaction exclusively to the SLG gene. On the other hand, it has been recently reported that mutations at the SRK gene, which would lead either to a non functional protein kinase in B. napus [ 10] or to the absence of SRK expression in B. oleracea and B. campestris [20], were associated with a self-compatibility character. This observation argues in favour of the SRK gene playing a key role in self-incompatibility.…”

Section: Particular Expression Of the Slg Gene In The $2 Haplotypementioning

confidence: 99%

Expression level of the SLG gene is not correlated with the self-incompatibility phenotype in the class II S haplotypes of Brassica oleracea

et al. 1995

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In Brassica, the S-locus glycoprotein (SLG) gene has been strongly implicated in the self-incompatibility reaction. Several alleles of this locus have been sequenced, and accordingly grouped as class I (corresponding to dominant S-alleles) and class II (recessive). We recently showed that a self-compatible (Sc) line of Brassica oleracea expressed a class II-like SLG (SLG-Sc) gene. Here, we report that the SLG-Sc glycoprotein is electrophoretically and immunochemically very similar to the recessive SLG-S15 glycoprotein, and is similarly expressed in stigmatic papillae. Moreover, by seed yield analysis, we observed that both alleles are associated with a self-compatibility response, in contrast with the other known recessive S haplotypes (S2 and S5). By genomic DNA blot analysis, we show the existence of molecular homologies between the Sc and S15 haplotypes, but demonstrate that they are not identical. On the other hand, we also report that the S2 haplotype expresses very low amounts of SLG glycoproteins, although it exhibits a self-incompatible phenotype. These results strongly question the precise role of the SLG gene in the molecular mechanisms that control the self-incompatibility reaction of Brassica.

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Section: Particular Expression Of the Slg Gene In The $2 Haplotypementioning

confidence: 99%

Expression level of the SLG gene is not correlated with the self-incompatibility phenotype in the class II S haplotypes of Brassica oleracea

et al. 1995

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“…Therefore, it is believed that SI is also regulated by genes other than the S-genes. A one-base deletion or an alternative transcript of the SRK gene located at the S-locus resulted in a low level of SI (Göring et al 1993;Tantikanjana et al 1993), and the m gene unlinked to the S-gene was related to the level of SI (Ikeda et al 1997). In addition, estimates of genetic factors have been performed using the wild population in order to gain an understanding of the evolution of the plant breeding system (Good and Stephenson 2002).…”

Section: Introductionmentioning

confidence: 97%

The effectiveness of insect-pollination test to evaluate the level of self-incompatibility and their genetic analysis in radish (Raphanus sativus L.)

Horisaki¹,

Тanaka²,

Niikura³

2003

Theor Appl Genet

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We have tried an insect-pollination self-incompatibility (SI) test to strictly evaluate the level of SI as a model for the actual F(1) seed production field using radish as experimental material. Twelve inbred lines, homozygous for the S-alleles, were used in the artificial self-pollination and the insect-pollination SI test. There was a positive correlation ( r = 0.606) between the results by these two methods. Some lines showed a low level of SI in the insect-pollination test despite showing a high level of SI in the artificial self-pollination test. On the other hand, no lines showing a low level of SI in artificial self-pollination had a high level of SI in insect-pollination. These results show that the insect-pollination SI test can be considered to be a more reliable and stricter method than the artificial self-pollination test with respect to an evaluation of SI levels. We have raised and analyzed an F(2) population and F(3) lines derived from an F(1) cross between a line showing a high level of SI (R00-04) and one showing a low level. The rate of self-seed settings of the F(2) population showed a binomial distribution. There were 39 high-level SI plants to 15 low-level SI plants. This result and F(3) progeny tests suggested that the high level of SI which R00-04 showed is controlled by a dominant gene.

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“…Both genes are expressed speci®cally in the stigma papillae cells and they are thought to be responsible for the female side of the SI phenotype when the communication between pistil and pollen begins (Nasrallah and Nasrallah 1993;Nasrallah et al 1994a). The expression of SLG and SRK is necessary for the SI function, as plants carrying mutated or down-regulated SLG or SRK genes are self-fertile Goring et al 1993;Nasrallah et al 1994b;Conner et al 1997;Stahl et al 1998). The current model for SI in Brassica proposes that the pollen-borne ligand interacts with SLG and SRK, activates the SRK protein kinase and thus triggers a signal cascade that eventually leads to the rejection of self-pollen (Nasrallah et al 1994a).…”

Section: Introductionmentioning

confidence: 98%

Transformation of Arabidopsis with a Brassica SLG/SRK region and ARC1 gene is not sufficient to transfer the self-incompatibility phenotype

Brugière

Cui

et al. 2000

Mol Gen Genet

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Self-incompatibility (SI) promotes outbreeding in flowering plants, and in Brassica SI is genetically controlled by the S locus. Self-incompatible Brassica and self-fertile Arabidopsis belong to the same crucifer family. In addition, a comparative analysis reveals a high degree of microsynteny between the B. campestris S locus and its homologous region in Arabidopsis--with the notable exception that the Brassica SI genes, SLG and SRK, are missing. Brassica ARC1 encodes a component of the SRK signal transduction pathway leading to self-pollen rejection, and no closely related ARC1 homolog has been identified in Arabidopsis. The purpose of the research reported here was to introduce Brassica SI components into Arabidopsis in an attempt to compensate for the missing genes and to investigate whether the SI phenotype can be transferred. Inserts of approximately 40 kb from the fosmid clones F20 and F22, which span the B. napus W1 SLG-SRK region, were cloned into the plant transformation vector pBIBAC2. Transgenic plants were generated that expressed the Brassica SI genes in the flower buds. In addition, the endogenous, SLG-like, gene AtS1 was not co-suppressed by the Brassica SLG transgene. No SI phenotype was observed among the T1 BIBAC2-F20 and BIBAC2-F22 transgenic plants. When the ARC1 gene was transformed into BIBAC2-F20 or BIBAC2-F22 plants, the resulting BIBAC2-F20-ARC1 and BIBAC2-F22-ARC1 plants still set seeds normally, and no rejection response was observed when self-incompatible B. napus W1 pollen was placed on BIBAC2-F20-ARC1 or BIBAC2-F22-ARC1 Arabidopsis stigmas. Taken together, our results suggest that complementing Arabidopsis genome with Brassica SLG, SRK and ARC1 genes is unlikely to be sufficient to transfer the SI phenotype.

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An S receptor kinase gene in self-compatible Brassica napus has a 1-bp deletion.

Cited by 142 publications

References 22 publications

Expression level of the SLG gene is not correlated with the self-incompatibility phenotype in the class II S haplotypes of Brassica oleracea

Expression level of the SLG gene is not correlated with the self-incompatibility phenotype in the class II S haplotypes of Brassica oleracea

The effectiveness of insect-pollination test to evaluate the level of self-incompatibility and their genetic analysis in radish (Raphanus sativus L.)

Transformation of Arabidopsis with a Brassica SLG/SRK region and ARC1 gene is not sufficient to transfer the self-incompatibility phenotype

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