2024
DOI: 10.1101/2024.06.20.599930
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An RNA interference approach for functional studies in the sea urchin and its use in analysis of Nodal signaling gradients

Keen Wilson,
Carl Manner,
Esther Miranda
et al.

Abstract: We report the first RNAi-based gene knockdowns in a sea urchin. Dicer-Substrate interfering RNAs (DsiRNAs) successfully disrupted expression of multiple transcripts in embryos of the sea urchinLytechinus variagatus,phenocopying known morpholino and inhibitor knockdowns. We describe our approach for the design of DsiRNAs most likely to target mRNAs of interest. An examination of DsiRNAs directed againstpks1, an enzyme necessary for pigment production, reveal destruction of thepks1mRNA without destroying the now… Show more

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