An RGA-Derived SCAR Marker Linked to PLRV Resistance from Solanum tuberosum ssp. andigena

Mihovilovich, E.; Aponte, Mariela; Lindqvist‐Kreuze, Hannele; Bonierbale, Merideth

doi:10.1007/s11105-013-0629-5

Cited by 20 publications

(19 citation statements)

References 42 publications

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“…A population of 167 primary dihaploids was generated from Alca Tarma via pollination with haploid inducers IVP101 or PL4 (Velásquez et al 2007;Mihovilovich et al 2014) over the course of two previous studies (Velásquez et al 2007;Mihovilovich et al 2014) . To identify true dihaploids, seeds lacking the homozygous dominant embryo spot seed marker present in both haploid inducer were grown, and seedlings were screened by guard cell chloroplast counts and root cell chromosome counts ( Fig.…”

Section: Induction Selection and Sequencing Of Dihaploidsmentioning

confidence: 99%

“…In potato ( Solanum tuberosum L.), the world's fourth most important crop in terms of calories consumed per person per day ( http://www.fao.org/faostat/en/#compare ), haploid seed can be routinely obtained via pollination with select haploid inducer varieties from the diploid S. tuberosum Andigenum Group (formerly S. tuberosum Phureja Group or S. phureja (Spooner et al 2014) . Such crosses with tetraploid potato (2n=4x=48) produce 2 n =2 x =24 dihaploids that can be used for genetic mapping (Kotch et al 1992;Pineda et al 1993;Ercolano et al 2004;Velásquez et al 2007;Mihovilovich et al 2014;Bartkiewicz et al 2018) . Additionally, these crosses produce hybrids that can be either triploid or tetraploid (Wagenvoort and Lange 1975;Hanneman and Ruhde 1978) , and can be identified as seed because they express a purple embryo spot, a dominant anthocyanin marker encoded by the haploid inducers that is expected to be absent in the dihaploids (Fig.1) (Hermsen and Verdenius 1973a) .…”

Section: Introductionmentioning

confidence: 99%

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Genomic Outcomes of Haploid Induction Crosses in Potato (Solanum tuberosumL.)

Amundson¹,

Ordoñez

Santayana

et al. 2019

Preprint

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The challenges of breeding autotetraploid potato ( Solanum tuberosum ) have motivated the development of alternative breeding strategies. A common approach is to obtain uniparental dihaploids from a tetraploid of interest through pollination with S. tuberosum Andigenum Group (formerly S. phureja ) cultivars. The mechanism underlying haploid formation of these crosses is unclear, and questions regarding the frequency of paternal DNA transmission remain. Previous reports described aneuploid and euploid progeny, which, in some cases, displayed genetic markers from the haploid inducer. Here, we surveyed a population of 167 presumed dihaploids for large-scale structural variation that would underlie chromosomal addition from the haploid inducer, and for small-scale introgression of genetic markers. In 19 progeny, we detected ten of the twelve possible trisomies and, in all cases, demonstrated the non-inducer parent origin of the additional chromosome. Deep sequencing indicated that occasional, short-tract signals appearing of haploid inducer origin were better explained as technical artifacts. Leveraging recurring CNV patterns, we documented sub-chromosomal dosage variation indicating segregation of polymorphic maternal haplotypes. Collectively, 52% of assayed chromosomal loci were classified as dosage variable. Our findings help elucidate the genomic consequences of potato haploid induction and suggest that most potato dihaploids will be free of residual pollinator DNA.

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Section: Induction Selection and Sequencing Of Dihaploidsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genomic Outcomes of Haploid Induction Crosses in Potato (Solanum tuberosumL.)

Amundson¹,

Ordoñez

Santayana

et al. 2019

Preprint

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“…andigena, resistance was mapped to the upper arm of chromosome V, which contains a known cluster of disease resistance genes (Vel asquez et al, 2007). This resistance locus was subsequently identified in other potato varieties (Mihovilovich et al, 2014).…”

Section: Potato Leafroll Virus: Limiting Its Own Movementmentioning

confidence: 99%

The enemy within: phloem‐limited pathogens

Bendix

Lewis

2017

Molecular Plant Pathology

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SUMMARYThe growing impact of phloem-limited pathogens on high-value crops has led to a renewed interest in understanding how they cause disease. Although these pathogens cause substantial crop losses, many are poorly characterized. In this review, we present examples of phloem-limited pathogens that include intracellular bacteria with and without cell walls, and viruses. Phloem-limited pathogens have small genomes and lack many genes required for core metabolic processes, which is, in part, an adaptation to the unique phloem environment. For each pathogen class, we present multiple case studies to highlight aspects of disease caused by phloem-limited pathogens. The pathogens presented include Candidatus Liberibacter asiaticus (citrus greening), Arsenophonus bacteria, Serratia marcescens (cucurbit yellow vine disease), Candidatus Phytoplasma asteris (Aster Yellows Witches' Broom), Spiroplasma kunkelii, Potato leafroll virus and Citrus tristeza virus. We focus on commonalities in the virulence strategies of these pathogens, and aim to stimulate new discussions in the hope that widely applicable disease management strategies can be found.

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“…Potato leafroll virus resistance has been identified in a number of wild relatives of cultivated potato including S. brevidens (Ahn et al 2001), S. chacoense (Brown and Thomas 1994), S. verrucosum (Carrasco et al 2000), and S. tuberosum ssp. Andigena Mihovilovich et al 2014;Velásquez et al 2007). Resistance has also been identified in genetic populations developed from diploid (Flis and Wasilewicz-Flis 1998;Swiezynski et al 1989) and tetraploid (Marczewski et al 2001;Marczewski et al 2004) breeding material.…”

Section: Molecular Markers For Marker Assisted Selectionmentioning

confidence: 99%

Development of Molecular Markers Closely Linked to the Potato Leafroll Virus Resistance Gene, Rlr etb , for use in Marker-Assisted Selection

et al. 2016

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Potato leafroll virus (PLRV) is a major pathogen of potato with worldwide impact on seed and commercial production of potato. In North America, the primary potato cultivars grown by industry are not resistant to PLRV and require the application of insecticides to control the aphid vector of PLRV, so as to prevent PLRV infection of the crop. The Solanum etuberosum-derived dominant gene Rlr etb confers resistance to PLRV and has been mapped to chromosome 4. The previous closest marker to Rlr etb , C2_At1g42990, was mapped 13.6 cM from the gene in a BC 3 population. Cleaved amplified polymorphic sequence markers were developed in the region surrounding C2_At1g42990 and mapped along with Rlr etb in 102 BC 4 progeny. Sixteen markers were identified surrounding Rlr etb , with flanking markers at 2.1 and 9.3 cM. Two markers, 1367-8a and DMB32-11, both 2.1 cM from Rlr etb , are shown to be well suited for marker assisted selection. The development of additional molecular markers more closely linked to Rlr etb will greatly facilitate its use in potato breeding and its continued introgression into cultivated potato for the development of PLRV-resistant cultivars.Resumen El virus del enrollamiento de la hoja de la papa (PLRV) es un patógeno importante de la papa con impacto mundial en la producción de semilla y en la producción comercial de la papa. En Norteamérica, las principales variedades de papa que se cultivan por la industria no son resistentes al PLRV y requieren de la aplicación de insecticidas para control del áfido vector del PLRV, así como para prevenir la infección del cultivo por PLRV. El gen dominante derivado de Solanum etuberosum, Rlretb, confiere resistencia al PLRV y ha sido ubicado en el cromosoma 4. El marcador previo más cercano al Rlretb, C2_At1g42990, fue ubicado a 13.6 cM del gen en una población BC3. Se desarrollaron marcadores fragmentados amplificados de secuencias polimórficas en la región que rodea a C2_At1g42990 y se les hizo un mapa junto con Rlretb en una progenie 102 BC4. Se identificaron 16 marcadores rodeando al Rlretb, con marcadores en los lados a 2.1 y 9.3 cM. Dos marcadores, 1367-8a y DMB32-11, ambos a 2.1 cM de Rlretb, se ha demostrado que son los apropiados para la selección asistida por marcadores. El desarrollo de marcadores moleculares adicionales más cercanamente ligados a Rlretb facilitará grandemente su uso en el mejoramiento de la papa así como su incorporación a papa cultivada para el desarrollo de variedades resistentes al PLRV.

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An RGA-Derived SCAR Marker Linked to PLRV Resistance from Solanum tuberosum ssp. andigena

Cited by 20 publications

References 42 publications

Genomic Outcomes of Haploid Induction Crosses in Potato (Solanum tuberosumL.)

Genomic Outcomes of Haploid Induction Crosses in Potato (Solanum tuberosumL.)

The enemy within: phloem‐limited pathogens

Development of Molecular Markers Closely Linked to the Potato Leafroll Virus Resistance Gene, Rlr etb , for use in Marker-Assisted Selection

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