An RFLP linkage map of Lycopersicon peruvianum

Ooijen, J.W. van; Sandbrink, J.M.; Vrielink, M.; Verkerk, Ruud; Zabel, P.; Lindhout, P.

doi:10.1007/bf00224531

Cited by 56 publications

(22 citation statements)

References 15 publications

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“…The use of molecular markers can facilitate tomato breeding by means of markerassisted selection (MAS) to improve agronomically important traits such as yield, fruit quality and disease resistance. In the last decade, molecular markers such as RFLP (Van Ooijen et al 1994;Sandbrink et al 1995;Stevens et al 1995), RAPD (Stevens et al 1995;Qian et al 2001), ISSR (Zietkiewicz et al 1994;Joshi et al 2000) and AFLP (Vos et al 1995) have been developed in tomato and other crops. However, the use of RFLP for breeding purposes is limited because it requires the use of radioactivity and is labour intensive; RAPD, ISSR and AFLP markers either identify only dominant alleles or are sensitive to PCR amplification conditions.…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of simple sequence repeat (SSR) markers and their use in determining relationships among Lycopersicon esculentum cultivars

2003

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The simple sequence repeat (SSR) or microsatellite marker is currently the preferred molecular marker due to its highly desirable properties. The aim of this study was to develop and characterize more SSR markers because the number of SSR markers currently available in tomato is very limited. Five hundred DNA sequences of tomato were searched for SSRs and analyzed for the design of PCR primers. Of the 158 pairs of SSR primers screened against a set of 19 diverse tomato cultivars, 129 pairs produced the expected DNA fragments in their PCR products, and 65 of them were polymorphic with the polymorphism information content (PIC) ranging from 0.09 to 0.67. Among the polymorphic loci, 2-6 SSR alleles were detected for each locus with an average of 2.7 alleles per locus; 49.2% of these loci had two alleles and 33.8% had three alleles. The vast majority (93.8%) of the microsatellite loci contained di- or tri-nucleotide repeats and only 6.2% had tetra- and penta-nucleotide repeats. It was also found that TA/AT was the most frequent type of repeat, and the polymorphism information content (PIC) was positively correlated with the number of repeats. The set of 19 tomato cultivars were clustered based on the banding patterns generated by the 65 polymorphic SSR loci. Since the markers developed in this study are primarily from expressed sequences, they can be used not only for molecular mapping, cultivar identification and marker-assisted selection, but for identifying gene-trait relations in tomato.

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Section: Introductionmentioning

confidence: 99%

Development and characterization of simple sequence repeat (SSR) markers and their use in determining relationships among Lycopersicon esculentum cultivars

2003

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“…Comparative genetic maps constructed from interspecific tomato populations show a high degree of colinearity between genomes of all tomato species. However, recombination is typically reduced after backcrossing to cultivated tomato (Rick 1969(Rick , 1971) and somewhat lower in male than in female gametes (de Vicente and Tanksley 1991;van Ooijen et al 1994).…”

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Homeologous Recombination in Solanum lycopersicoides Introgression Lines of Cultivated Tomato

Canady¹,

Ji²,

Chetelat

2006

Genetics

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A library of ''introgression lines'' containing Solanum lycopersicoides chromosome segments in the genetic background of cultivated tomato (Lycopersicon esculentum) was used to study factors affecting homeologous recombination. Recombination rates were estimated in progeny of 43 heterozygous introgressions and whole-chromosome substitution lines, together representing 11 of the 12 tomato chromosomes. Recombination within homeologous segments was reduced to as little as 0-10% of expected frequencies. Relative recombination rates were positively correlated with the length of introgressed segments on the tomato map. The highest recombination (up to 40-50% of normal) was observed in long introgressions or substitution lines. Double-introgression lines containing two homeologous segments on opposite chromosome arms were synthesized to increase their combined length. Recombination was higher in the double than in the single segment lines, despite a preference for crossovers in the region of homology between segments. A greater increase in homeologous recombination was obtained by crossing the S. lycopersicoides introgression lines to L. pennellii-a phylogenetically intermediate species-or to L. esculentum lines containing single L. pennellii segments on the same chromosome. Recombination rates were highest in regions of overlap between S. lycopersicoides and L. pennellii segments. The potential application of these results to breeding with introgression lines is discussed.

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“…Furthermore, Lycopersicon has many useful attributes for population genetic studies. All nine species are diploids and there are extensive, well-documented collections from natural populations of all species in the group (http://tgrc.ucdavis.edu); also Lycopersicon esculentum, the cultivated tomato, is a model species for plant genetics and detailed genetic maps exist for several others (e.g., Tanksley et al 1992;van Ooijen et al 1994;Monforte and Tanksley 2000).…”

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confidence: 99%

Natural Variation in the Pto Pathogen Resistance Gene Within Species of Wild Tomato (Lycopersicon). I. Functional Analysis of Pto Alleles

Rose

Langley

Bernal³

et al. 2005

Genetics

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Disease resistance to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) in the cultivated tomato, Lycopersicon esculentum, and the closely related L. pimpinellifolium is triggered by the physical interaction between plant disease resistance protein, Pto, and the pathogen avirulence protein, AvrPto. To investigate the extent to which variation in the Pto gene is responsible for naturally occurring variation in resistance to Pst, we determined the resistance phenotype of 51 accessions from seven species of Lycopersicon to isogenic strains of Pst differing in the presence of avrPto. One-third of the plants displayed resistance specifically when the pathogen expressed AvrPto, consistent with a gene-for-gene interaction. To test whether this resistance in these species was conferred specifically by the Pto gene, alleles of Pto were amplified and sequenced from 49 individuals and a subset (16) of these alleles was tested in planta using Agrobacterium-mediated transient assays. Eleven alleles conferred a hypersensitive resistance response (HR) in the presence of AvrPto, while 5 did not. Ten amino acid substitutions associated with the absence of AvrPto recognition and HR were identified, none of which had been identified in previous structure-function studies. Additionally, 3 alleles encoding putative pseudogenes of Pto were isolated from two species of Lycopersicon. Therefore, a large proportion, but not all, of the natural variation in the reaction to strains of Pst expressing AvrPto can be attributed to sequence variation in the Pto gene.

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An RFLP linkage map of Lycopersicon peruvianum

Cited by 56 publications

References 15 publications

Development and characterization of simple sequence repeat (SSR) markers and their use in determining relationships among Lycopersicon esculentum cultivars

Development and characterization of simple sequence repeat (SSR) markers and their use in determining relationships among Lycopersicon esculentum cultivars

Homeologous Recombination in Solanum lycopersicoides Introgression Lines of Cultivated Tomato

Natural Variation in the Pto Pathogen Resistance Gene Within Species of Wild Tomato (Lycopersicon). I. Functional Analysis of Pto Alleles

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