An R2R3‐<scp>MYB</scp> transcription factor regulates carotenoid pigmentation in <i>Mimulus lewisii</i> flowers

Sagawa, Janelle M.; Stanley, Lauren E.; LaFountain, Amy M.; Frank, Harry A.; Liu, Chang; Yuan, Yao‐Wu

doi:10.1111/nph.13647

Cited by 153 publications

(119 citation statements)

References 65 publications

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“…The evidence to date suggests that regulation of carotenoid production in fruit occurs as part of a wider gene regulatory process, rather than through a specific transcriptional complex such as the MBW complex for anthocyanin gene regulation. However, an R2R3MYB belonging to the AtMYB21 clade has recently been suggested to be a direct activator of carotenoid biosynthetic gene expression in Mimulus lewisii petals, being the gene disrupted in the Reduced Carotenoid Pigmentation 1 (RCP1) mutant (Sagawa et al 2015).…”

Section: Regulation Of Carotenoid Production In Kiwifruitmentioning

confidence: 99%

Genetics of Pigment Biosynthesis and Degradation

Montefiori

Pilkington

Davies

et al. 2016

Compendium of Plant Genomes

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Within the genus Actinidia, there is large variability in fruit skin and flesh colour. The most familiar kiwifruit, A. chinensis var. deliciosa 'Hayward', has flesh of bright emerald green. Although many species have live, coloured fruit skins, most commercial cultivars have a dead, brown fruit skin. Hidden underneath the skin is a diversity and range of flesh colours which are characteristic of the species or specific genotypes. From a commercial point of view, flesh colour has become a particularly important feature which distinguishes the fruit and new cultivars in the market. In today's market, there is a large number of cultivars with a range of flesh colours grouped into three main categories: green, yellow and red. The red-fleshed cultivars are a recent addition and have generated great interest throughout the industry worldwide. It is also the distinctive feature of the genotype used to build the reference genome for A. chinensis var. chinensis (Huang et al., 2013). Fruit ColourAnthocyanins, carotenoids and chlorophylls are responsible for almost all fruit coloration. Plant breeding and domestication have produced numerous fruit where the levels and distribution of pigments vary widely, changing during development and in response to the environment. The accumulation of pigments in fruit is an important indicator of ripeness, quality and value. These pigments also differentiate cultivars for consumers, as well as being implicated in the health attributes of our foods. A highly pigmented fruit has become perceived by consumers as conferring

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Section: Regulation Of Carotenoid Production In Kiwifruitmentioning

confidence: 99%

Genetics of Pigment Biosynthesis and Degradation

Montefiori

Pilkington

Davies

et al. 2016

Compendium of Plant Genomes

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“…An R2R3-Myb transcription factor, Reduced Carotenoid Pigmentation 1 ( RCP1 ), has been the only transcription factor identified to be involved in flower-specific carotenoid biosynthesis [95]. Our analyses identified 9 transcripts with similarity to RCP1 (Additional file 11).…”

Section: Discussionmentioning

confidence: 97%

“…The role of R2R3-Myb transcription factors in regulating various steps of the ABP has been well studied in numerous plants [86, 91, 95] and the possible role of these transcription factors in Achimenes will need to be studied further. We identified putative proteins in Achimenes with high-similarity to R2R3-Mybs that have experimental evidence indicating their role in regulating anthocyanin accumulation (Additional file 11).…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analyses of flower development in four species of Achimenes (Gesneriaceae)

Roberts

Roalson

2017

BMC Genomics

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BackgroundFlowers have an amazingly diverse display of colors and shapes, and these characteristics often vary significantly among closely related species. The evolution of diverse floral form can be thought of as an adaptive response to pollination and reproduction, but it can also be seen through the lens of morphological and developmental constraints. To explore these interactions, we use RNA-seq across species and development to investigate gene expression and sequence evolution as they relate to the evolution of the diverse flowers in a group of Neotropical plants native to Mexico—magic flowers (Achimenes, Gesneriaceae).ResultsThe assembled transcriptomes contain between 29,000 and 42,000 genes expressed during development. We combine sequence orthology and coexpression clustering with analyses of protein evolution to identify candidate genes for roles in floral form evolution. Over 25% of transcripts captured were distinctive to Achimenes and overrepresented by genes involved in transcription factor activity. Using a model-based clustering approach we find dynamic, temporal patterns of gene expression among species. Selection tests provide evidence of positive selection in several genes with roles in pigment production, flowering time, and morphology. Combining these approaches to explore genes related to flower color and flower shape, we find distinct patterns that correspond to transitions of floral form among Achimenes species.ConclusionsThe floral transcriptomes developed from four species of Achimenes provide insight into the mechanisms involved in the evolution of diverse floral form among closely related species with different pollinators. We identified several candidate genes that will serve as an important and useful resource for future research. High conservation of sequence structure, patterns of gene coexpression, and detection of positive selection acting on few genes suggests that large phenotypic differences in floral form may be caused by genetic differences in a small set of genes. Our characterized floral transcriptomes provided here should facilitate further analyses into the genomics of flower development and the mechanisms underlying the evolution of diverse flowers in Achimenes and other Neotropical Gesneriaceae.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3623-8) contains supplementary material, which is available to authorized users.

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“…Carotenoid production can be elevated through genetic engineering by the identification of transcription factors regulating the expression of structural genes in carotenoid biosynthesis (Sagawa et al, 2015). As shown in Table 5, GRMZM2G045748, a transcription factor belonging to the MYB family, is co-expressed only with GRMZM2G300348_T01 under conditions of environmental stress.…”

Section: Discussionmentioning

confidence: 99%

“…Identification of transcription factors regulating the expression of structural genes in the carotenoid biosynthesis pathway provides knowledge on gene expression for the potential enhancement of carotenoid production in plants via genetic engineering (Sagawa et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

In silico identification of transcription factors associated with the biosynthesis of carotenoids in corn ( Zea mays L. )

Zinati¹,

Nazari²,

Bagnaresi³

et al. 2017

bta

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Carotenoids, a diverse group of colorful pigments, contribute to the development, light harvesting and photoprotection in plants as well as human health. Due to the interesting properties of carotenoids, enhanced carotenoid biosynthesis has been of ongoing interest. Recent advances in computational biology and bioinformatics make it more feasible to understand the transcriptional regulatory network underlying carotenoid biosynthesis.Studies on carotenoid biosynthesis in corn (Zea mays L.) have indicated the pivotal role of the phytoene synthase gene PSY1 (accession: GRMZM2G300348) in endosperm color and carotenoid accumulation in corn kernels.Computational approaches such as Genomatix, PlantPAN, PlantCARE, PlantTFDB and IGDE6 have been used for promoter prediction, regulatory features and transcription factor identification, as well as pairwise promoter comparisons. Four transcripts have been identified for the PSY1 gene. Based on Genomatix and PlantPAN, the promoter predicted for GRMZM2G300348_T01 was different from that predicted for the other three transcripts (GRMZM2G300348_T02, GRMZM2G300348_T03 and GRMZM2G300348_T04). The results indicated that the promoter of GRMZM2G300348_T01 has more diverse motifs involved in hormonal/environmental stress responses. The most significant result obtained from this study is the discovery of two transcription factors belonging to the HB family that are co-expressed with all four transcripts of PSY1 under environmental stresses. It is, therefore, likely that these transcription factors may act as critical regulators of PSY1 gene expression in corn. Identification of the proteins acting upstream of PSY1 within corn will shed light on the fine tuning of PSY1 expression regulation. Such an understanding would also contribute to metabolic engineering aimed at enhanced carotenoid biosynthesis.

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An R2R3‐MYB transcription factor regulates carotenoid pigmentation in Mimulus lewisii flowers

Cited by 153 publications

References 65 publications

Genetics of Pigment Biosynthesis and Degradation

Genetics of Pigment Biosynthesis and Degradation

Comparative transcriptome analyses of flower development in four species of Achimenes (Gesneriaceae)

In silico identification of transcription factors associated with the biosynthesis of carotenoids in corn ( Zea mays L. )

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