An oviduct-specific and enhancer-like element resides at about −3000 in the chicken ovalbumin gene

Park, Hyi Man; Sanders, Michel M.; Suzuki, Takayuki; Muramatsu, Tatsuo

doi:10.1016/j.biochi.2006.06.015

Cited by 6 publications

(7 citation statements)

References 17 publications

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“…Its expression is strictly limited to the tubular gland cells in the magnum portion of the estrogen-stimulated oviduct. The region from -900 to +9 directs expression in primary tubular gland cell cultures but not in other tissue culture cells, although a region between -3100 and -2800 might contribute to tissue specificity in vivo (Park et al, 2006). Unfortunately, transgenic chicken technology is not available to assess the elements required for oviduct-specific expression in vivo .…”

Section: Discussionmentioning

confidence: 99%

“…This implies that tissue-specific activators, repressors, and/or coregulators exist that allow transcription only in estrogen-stimulated oviduct and repress it in all other contexts. Data suggest that estrogen-stimulated oviduct contains proteins not present in other tissues, although their identities are unknown (Upadhyay et al, 1999; Park et al, 2006). The goal of these experiments was to gain a better understanding of the tissue-specific regulation of Ov .…”

Section: Introductionmentioning

confidence: 99%

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Interferon regulatory factors (IRFs) repress transcription of the chicken ovalbumin gene

Dougherty¹,

Park²,

Sanders³

2009

Gene

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Although the ovalbumin (Ov) gene has served as a model to study tissue-specific, steroid hormoneinduced gene expression in vertebrates for decades, the mechanisms responsible for regulating this gene remain elusive. Ov is repressed in non-oviduct tissue and in estrogen-deprived oviduct by a strong repressor site located from -130 to -100 and designated CAR for COUP-TF adjacent repressor. The goal of this study was to identify the CAR binding protein(s). A transcription factor database search revealed that a putative interferon-stimulated response element (ISRE), which binds interferon regulatory factors (IRFs), is located in this region. Gel mobility shift assays demonstrated that the protein(s) binding to the CAR site is recognized by an IRF antibody and that mutations in the ISRE abolish that binding. In hopes of identifying the IRF(s) responsible for the tissue-specific regulation of Ov, mRNA levels for IRFs-4, -8, and -10 were measured in seven tissues from chicks treated with or without estrogen. PCR experiments showed that both IRF-8 and -10 are expressed in all chick tissues tested whereas IRF-4 has a much more limited expression pattern. Transfection experiments with OvCAT (chloramphenicol acetyltransferase) reporter constructs demonstrated that both IRF-4 and IRF-10 are capable of repressing the Ov gene even in the presence of steroid hormones and that nucleotides in the ISRE are required for repression. These experiments indicate that the repressor activity associated with the CAR site is mediated by IRF family members and suggest that IRF members also repress Ov in non-oviduct tissues.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interferon regulatory factors (IRFs) repress transcription of the chicken ovalbumin gene

Dougherty¹,

Park²,

Sanders³

2009

Gene

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“…This technique can be applied to any chick tissue, including the oviduct (Park & Muramatsu 1999; Takami et al . 2002; Park et al . 2006), testis (Sugihara et al .…”

Section: Introductionmentioning

confidence: 99%

“…In this gene delivery method, electric pulses are locally applied after injecting DNA into the target tissue. This technique can be applied to any chick tissue, including the oviduct (Park & Muramatsu 1999;Takami et al 2002;Park et al 2006), testis (Sugihara et al 2000a), nerve (Delfini & Duprez 2004), brain (Yamaguchi et al 2007), optic tectum (Chen et al 2004;Luo & Redies 2005), spinal cord and heart (Harrison et al 1998). However, electroporation into the skeletal muscle of neonatal chicks has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

In vivo gene transfer into skeletal muscle of neonatal chicks by electroporation

Ijiri

Saegusa

Matsubara

et al. 2011

Animal Science Journal

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Chicks (Gallus gallus domesticus) show considerable growth of skeletal muscle during the neonatal period. The in vivo gene transfer method is useful for studying gene function and can be employed to elucidate the molecular mechanisms of skeletal muscle growth in chicks. We evaluated the following conditions for gene transfer to the skeletal muscle of neonatal chicks by electroporation: (i) voltage; (ii) age of the chick; (iii) plasmid DNA injected amount; and (iv) duration of gene expression. The results obtained from this study indicate that the most efficient gene transfer condition was as follows: 75 µg of plasmid DNA encoding β-galactosidase was injected into the gastrocnemius muscle of chicks at 4 days of age electroporated at 50 V/cm. In addition, peak transferred gene expression was observed from 3 days to 5 days after electroporation. Our results provide optimal electroporation conditions for elucidating the gene function related to skeletal muscle growth and development in neonatal chicks.

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“…Thorough analysis has identified additional oviduct-specific regulatory elements upstream of the ovalbumin coding sequences, including a tissue-specific element, the chicken oviduct-specific and enhancer-like region (COSE) (Park et al 2006) and EREs (Lilico et al 2007). Further studies on these and other elements, and how they interact in vivo to drive tissue-specific expression, will be required in order to generate reproducibly transgenic chickens with high-level tissue-specific expression for use as bioreactors.…”

Section: Transgene Expression Constructsmentioning

confidence: 99%

Primordial germ cells (PGCs) as a tool for creating transgenic chickens

Chojnacka-Puchta¹,

Kasperczyk²,

Płucienniczak³

et al. 2012

Polish Journal of Veterinary Sciences

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The transgenic chicken has great potential as a bioreactor for the production of valuable pharmaceutical proteins, notably in the oviduct/egg. Whereas conventional transgenic approaches have significant limitations in this species, an alternative approach employing primordial germ cells (PGCs), the progenitor cells to ova and spermatozoa, has now been successfully applied to the insertion of exogenous genes into birds. Recent developments in manipulating avian embryos make it possible to produce germline chimeras derived from transferred PGCs. In this review we describe the migration pathway of chicken PGCs during early development. We then summarize different methods for the isolation of PGCs and the diversity of techniques used to introduce genes into these cells. Finally, we describe an in vitro assay for testing tissue-specific vectors designed to express heterologous proteins in transgenic chickens.

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An oviduct-specific and enhancer-like element resides at about −3000 in the chicken ovalbumin gene

Cited by 6 publications

References 17 publications

Interferon regulatory factors (IRFs) repress transcription of the chicken ovalbumin gene

Interferon regulatory factors (IRFs) repress transcription of the chicken ovalbumin gene

In vivo gene transfer into skeletal muscle of neonatal chicks by electroporation

Primordial germ cells (PGCs) as a tool for creating transgenic chickens

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