An overview on the protective effects of ellagic acid against heavy metals, drugs, and chemicals

Jamshidi, Zahra; Roohbakhsh, Ali; Karimi, Gholamreza

doi:10.1002/fsn3.3704

Cited by 3 publications

(3 citation statements)

References 129 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…EA reduces lipid peroxidation, serves as a ROS scavenger, and positively affects mediators that are pro-oxidant and anti-inflammatory. Treatment with EA could inhibit Cd-induced oxidative injury in rat primary astrocytes [42]. EA dramatically boosted the activity of antioxidant enzymes, such as GPx, SOD, and CAT, in rats exposed to Hg.…”

Section: Discussionmentioning

confidence: 91%

See 1 more Smart Citation

Evaluation of the Potential Protective Effect of Ellagic Acid against Heavy Metal (Cadmium, Mercury, and Lead) Toxicity in SH-SY5Y Neuroblastoma Cells

Mallamaci,

Barbarossa,

Carocci

et al. 2024

Foods

View full text Add to dashboard Cite

Ellagic acid (EA), a polyphenolic constituent of plant origin, has been thoroughly investigated for its hypothesised pharmacological properties among which antioxidant and neuroprotective activities are included. The present study was designed to explore whether EA could attenuate heavy metal (cadmium, mercury, and lead)-induced neurotoxicity in SH-SY5Y cells, which were utilized as a model system for brain cells. MTT and LDH assays were performed to examine the viability of the SH-SY5Y cells after exposure to Cd, Hg, and Pb (either individually or in combination with EA) as well as the effects of necrotic cell death, respectively. Furthermore, 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), a cell-based assay, was performed to determine whether EA could protect SH-SY5Y from heavy metal-induced oxidative stress. Results allowed us to assess the capability of EA to enhance the number of viable SH-SY5Y cells after exposure to heavy metal toxicity. Pre-treatment with EA showed a considerable, concentration-dependent, cytoprotective effect, particularly against Cd2+-induced toxicity. This effect was confirmed through the reduction of LDH release after the simultaneous cell treatment with Cd2+ and EA compared with Cd2+-treated cells. Furthermore, a significant, concentration-dependent decrease in reactive oxygen species (ROS) production, induced by H2O2 or heavy metals, was observed in the same model. Overall, the obtained results provide further insight into the protective role of EA against heavy metal-induced neurotoxicity and oxidative stress, thus indicating the potential beneficial effects of the consumption of EA-rich foods. However, to confirm its effects, well-designed human randomized controlled trials are needed to fill the existing gap between experimental and clinical research.

show abstract

Section: Discussionmentioning

confidence: 91%

“…[39][40][41]. Results of a recent study have shown that EA can alleviate the damaging effects of heavy metals, especially those of Pb, on the human body [42]. Oral EA treatment in rats has been shown to provide protection against Pb-induced nephrotoxicity by suppressing oxidative stress, inflammation, apoptosis, and autophagy [43].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Potential Protective Effect of Ellagic Acid against Heavy Metal (Cadmium, Mercury, and Lead) Toxicity in SH-SY5Y Neuroblastoma Cells

Mallamaci,

Barbarossa,

Carocci

et al. 2024

Foods

View full text Add to dashboard Cite

show abstract

“…It has been reported that functional properties of EA towards the various action towards like antioxidant enzymes (8, 9). like catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) (51, 52). In another study demonstrated suppression of cyclooxygenase (COX-2) and nuclear factor-kappa B (NF-кB) (53).…”

Section: Discussionmentioning

confidence: 99%

Ellagic acid: a potential inhibitor of enhancer of zeste homolog-2 and protein arginine methyltransferase-5

Nalla,

Chatterjee,

Poyya

et al. 2024

Preprint

View full text Add to dashboard Cite

Dysregulation of epigenetic processes, characterized by aberrant DNA methylation patterns and histone modifications, is a hallmark of cancer, driving its initiation, progression, and metastasis by silencing tumor suppressor genes or activating oncogenes. Perturbations in histone modifications such as H3K27me3 by EZH2 and H4R3me2s by PRMT5 play significant roles in these epigenetic alterations, disrupting normal gene expression and facilitating oncogene activation while suppressing tumor suppressor genes. Consequently, inhibitors targeting enzymes involved in DNA methylation, histone modification, or chromatin remodeling, such as PRMTs and PRC complexes, are promising anti-cancer agents, with several undergoing pre-clinical and clinical trials. Our screening of a phytochemical library revealed ellagic acid as an effective inhibitor of both EZH2 and PRMT5. Ellagic acid interacts strongly with the EZH2 and PRMT5:MEP50 complex, binding to their active sites through π-cation interactions and hydrogen bonds. Surface Plasmon Resonance study confirmed potent binding affinities of ellagic acid, with KD values of 3.28E-06 and 6.54E-05 for EZH2 and PRMT5:MEP50 respectively. In-vitro assays validated inhibitory effects on EZH2 and PRMT5:MEP50 by reducing the levels of their catalytic products H3K27me3 and H4R3me2s respectively and induction of autophagy and apoptosis. Further In-vivo studies using mouse xenografts further demonstrated significant tumor size reductions upon oral administration of ellagic acid, with decreased expression of the proliferative marker ki67 and histone repressive marks. Taken together we showed that inhibition of EZH2 and PRMT5:MEP50 by ellagic acid could be used to develop breast cancer therapeutic drug.

show abstract

Effect of Cucumis melo var. agrestis Naudin on doxorubicin-induced cardiotoxicity in rats

Sarman

2024

Cukurova Medical Journal

View full text Add to dashboard Cite

Purpose: Doxorubicin (DOX), a chemotherapeutic antibiotic, induces toxicity by also targeting non-cancerous cells. Cucumis melo var. agrestis Naudin (CM), a plant belonging to the Cucurbitaceae family with high antioxidant content, is examined in this study for its potential impact on DOX-induced cardiac damage at different doses. Materials and Methods: 30 male rats were randomly divided into 5 groups, with 6 animals in each group: Control group, which received distilled water by gavage for 10 days, and intraperitoneal (i.p.) normal saline application on the 5th day of the experiment. The DOX group consisted of rats receiving a single i.p. dose of 15 mg/kg DOX on the 5th day of the experiment. Rats receiving a single intraperitoneal dose of 15 mg/kg DOX on the 5th day of the experiment were subjected to gavage for 10 days with doses of 100 mg/kg (DOX+CM100), 250 mg/kg (DOX+CM250), and 500 mg/kg (DOX+CM500) of CM, respectively. 24 hours after the last drug administration, the experimental animals were sacrificed under anesthesia. Heart tissue was examined histochemically and immunohistochemically. Results: At the end of the experiment, histopathological examination of the heart tissue; Compared to the control group, histolopathological findings such as degeneration of muscle fibers, vacuole-like structures between muscle fibers, congestion in vessels, and edema between collaterals were observed in the DOX group. These findings significantly decreased in the DOX+CM250 treatment group. While an increase in Caspase-3, HSP 70 and NF-κB-p65 immunoreactivities was observed in the DOX group (+++); In the DOX+CM250 group, these findings decreased significantly (+). Conclusion: DOX accelerated the apoptotic process, increased intracellular and oxidative stress, and triggered an inflammatory response, as demonstrated histochemically and immunohistochemically. CM administered at a dose of 250 mg/kg expedited cardiac remodeling.

show abstract

An overview on the protective effects of ellagic acid against heavy metals, drugs, and chemicals

Cited by 3 publications

References 129 publications

Evaluation of the Potential Protective Effect of Ellagic Acid against Heavy Metal (Cadmium, Mercury, and Lead) Toxicity in SH-SY5Y Neuroblastoma Cells

Evaluation of the Potential Protective Effect of Ellagic Acid against Heavy Metal (Cadmium, Mercury, and Lead) Toxicity in SH-SY5Y Neuroblastoma Cells

Ellagic acid: a potential inhibitor of enhancer of zeste homolog-2 and protein arginine methyltransferase-5

Effect of Cucumis melo var. agrestis Naudin on doxorubicin-induced cardiotoxicity in rats

Contact Info

Product

Resources

About