An overview on marine cellulolytic enzymes and their potential applications

Barzkar, Noora; Sohail, Muhammad

doi:10.1007/s00253-020-10692-y

Cited by 40 publications

(29 citation statements)

References 141 publications

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“…It should be noted that these Demequinaceae reads were not assigned to cellulose degraders in this system because Demequinaceae was a newly identified family (Fig 4). Although cellulose degradation by fungi in mangrove sediments has been described [35], fungi were not detected through 16S metagenome analysis in this study. However, to the best of our knowledge, little is established about their symbiosis with crabs.…”

Section: Metabolism Of Carboncontrasting

confidence: 57%

“…Cellulase is a general term for cellulose-degrading enzymes, which can be classified into three types based on the mode of enzymatic action and substrate specificity: endoglucanases (EC 3.2.1.4), exoglucanases (EC 3.2.1.74 and 3.2.1.91), and β-glucosidases (EC 3.2.1.21) [34]. In mangrove sediments, several microbes and genes encoding enzymes have been identified to be involved in cellulose degradation, which can promote litter degradation [35]. In addition, endogenous enzymes in the digestive organs have been identified in a wide variety of invertebrates [34,36] including mangrove crabs [37][38][39].…”

Section: Introductionmentioning

confidence: 99%

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Mangrove crab intestine and habitat sediment microbiomes cooperatively work on carbon and nitrogen cycling

et al. 2021

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Mangrove ecosystems, where litter and organic components are degraded and converted into detrital materials, support rich coastal fisheries resources. Sesarmid (Grapsidae) crabs, which feed on mangrove litter, play a crucial role in material flow in carbon-rich and nitrogen-limited mangrove ecosystems; however, the process of assimilation and conversion into detritus has not been well studied. In this study, we performed microbiome analyses of intestinal bacteria from three species of mangrove crab and five sediment positions in the mud lobster mounds, including the crab burrow wall, to study the interactive roles of crabs and sediment in metabolism. Metagenome analysis revealed species-dependent intestinal profiles, especially in Neosarmatium smithi, while the sediment microbiome was similar in all positions, albeit with some regional dependency. The microbiome profiles of crab intestines and sediments were significantly different in the MDS analysis based on OTU similarity; however, 579 OTUs (about 70% of reads in the crab intestinal microbiome) were identical between the intestinal and sediment bacteria. In the phenotype prediction, cellulose degradation was observed in the crab intestine. Cellulase activity was detected in both crab intestine and sediment. This could be mainly ascribed to Demequinaceae, which was predominantly found in the crab intestines and burrow walls. Nitrogen fixation was also enriched in both the crab intestines and sediments, and was supported by the nitrogenase assay. Similar to earlier reports, sulfur-related families were highly enriched in the sediment, presumably degrading organic compounds as terminal electron acceptors under anaerobic conditions. These results suggest that mangrove crabs and habitat sediment both contribute to carbon and nitrogen cycling in the mangrove ecosystem via these two key reactions.

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Section: Metabolism Of Carboncontrasting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Mangrove crab intestine and habitat sediment microbiomes cooperatively work on carbon and nitrogen cycling

et al. 2021

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“…Even though these enzymes have been characterized from a wide range of different sources, microbial fibrinolytic enzymes are considered attractive tools due to their features, such as enhanced specificity [8], low production cost [8], comparatively high yield [28] and the possibility to be genetically modified by recombinant DNA technology and protein engineering approaches [29]. Marine ecosystems serve as a reservoir of microorganisms producing important therapeutic metabolites, especially enzymes [30][31][32][33][34][35][36][37][38][39], but they remain largely unexplored to date. Due to the wide biodiversity of the marine environment, marine microorganisms can provide a diverse array of enzymes for biotechnological development, with possible improved pharmacological properties and lesser side effects [40].…”

Section: Introductionmentioning

confidence: 99%

Marine Microbial Fibrinolytic Enzymes: An Overview of Source, Production, Biochemical Properties and Thrombolytic Activity

2022

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Cardiovascular diseases (CVDs) have emerged as a major threat to global health resulting in a decrease in life expectancy with respect to humans. Thrombosis is one of the foremost causes of CVDs, and it is characterized by the unwanted formation of fibrin clots. Recently, microbial fibrinolytic enzymes due to their specific features have gained much more attention than conventional thrombolytic agents for the treatment of thrombosis. Marine microorganisms including bacteria and microalgae have the significant ability to produce fibrinolytic enzymes with improved pharmacological properties and lesser side effects and, hence, are considered as prospective candidates for large scale production of these enzymes. The current review presents an outline regarding isolation sources, production, features, and thrombolytic potential of fibrinolytic biocatalysts from marine microorganisms identified so far.

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“…Marine environments are home to complex and diverse microorganisms that are yet to be discovered through appropriate microbial investigations. Currently, marinederived halophilic bacteria are being explored to harness their novel enzymes and bioactive compounds as substitutes for many industrial applications [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

“…The complete degradation of lignocellulose involves a combination of numerous enzymes that can be categorised into three types based on their substrate specificities: cellulase, hemicellulase, and ligninolytic enzymes [34]. Cellulases, including β-glucosidase (EC 3.2.1.21), endoglucanase (EC 3.2.1.4), and exoglucanase (EC 3.2.1.91), are a group of enzymes that target the typical plant cell wall composed of cellulose [4]. β-Glucanases, such as endo-1,3(4)-β-glucanase (EC 3.2.1.6) and licheninase (EC 3.2.…”

Section: Introductionmentioning

confidence: 99%

Genome Analysis of Celeribacter sp. PS-C1 Isolated from Sekinchan Beach in Selangor, Malaysia, Reveals Its β-Glucosidase and Licheninase Activities

et al. 2022

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A halophilic marine bacterial strain, PS-C1, was isolated from Sekinchan beach in Selangor, Malaysia. The 16S rRNA gene sequence analysis indicated that strain PS-C1 was associated with the genus Celeribacter. To date, there have been no reports on enzymes from the genus Celeribacter. The present study reports on the cellular features of Celeribacter sp. PS-C1, its annotated genome sequence, and comparative genome analyses of Celeribacter glycoside hydrolase (GH) enzymes. The genome of strain PS-C1 has a size of 3.87 Mbp and a G+C content of 59.10%, and contains 3739 protein-coding genes. Detailed analysis using the Carbohydrate-Active enZYmes (CAZy) database revealed that Celeribacter genomes harboured at least 12 putative genes encoding industrially important GHs that are grouped as cellulases, β-glucanases, hemicellulases, and starch-degrading enzymes. Herein, the potential applications of these enzymes are discussed. Furthermore, the activities of two types of GHs (β-glucosidase and licheninase) in strain PS-C1 were demonstrated. These findings suggest that strain PS-C1 could be a reservoir of novel GH enzymes for lignocellulosic biomass degradation.

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An overview on marine cellulolytic enzymes and their potential applications

Cited by 40 publications

References 141 publications

Mangrove crab intestine and habitat sediment microbiomes cooperatively work on carbon and nitrogen cycling

Mangrove crab intestine and habitat sediment microbiomes cooperatively work on carbon and nitrogen cycling

Marine Microbial Fibrinolytic Enzymes: An Overview of Source, Production, Biochemical Properties and Thrombolytic Activity

Genome Analysis of Celeribacter sp. PS-C1 Isolated from Sekinchan Beach in Selangor, Malaysia, Reveals Its β-Glucosidase and Licheninase Activities

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