An overview on forensic analysis devoted to analytical chemists

Castillo-Peinado, L.S.; Castro, M. D. Luque de

doi:10.1016/j.talanta.2017.01.087

Cited by 40 publications

(17 citation statements)

References 97 publications

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“…Fingerprints are the product of the contact of papillary ridges—present on human beings’ fingertips, palms, and soles—with a surface; this interaction transfers sweat and skin components to the material, leaving a particular mark (). Many studies describe the composition of such residues as a complex mixture of numerous fatty acids, inorganic compounds, amino acids, and over 400 polypeptides (). From the chemical point of view, a fingerprint is a complex emulsion composed by water (generally between 20% and 70% in freshly deposited fingermarks), endogenous organic, and inorganic compounds secreted by the eccrine glands, sebaceous components (mostly lipids excreted from sebaceous glands, incorporated into the mixture by touching face, hair, and neck), and several exogenous contaminants that adhere to the surface of skin, such as blood, cosmetics, drugs, and food fats ().…”

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confidence: 99%

Methodologies Applied to Fingerprint Analysis

González

Gorziza

Mariotti

et al. 2020

Journal of Forensic Sciences

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This systematic review deals with the last 10 years of research in analytical methodologies for the analysis of fingerprints, regarding their chemical and biological constituents. A total of 123 manuscripts, which fit the search criteria defined using the descriptor “latent fingermarks analysis,” were selected. Its main instrumental areas (mass spectrometry, spectroscopy, and innovative methods) were analyzed and summarized in a specific table, highlighting its main analytical parameters. The results show that most studies in this field use mass spectrometry to identify the constituents of fingerprints, both to determine the chemical profile and for aging. There is also a marked use of mass spectrometry coupled with chromatographic methods, and it provides accurate results for a fatty acid profile. Additional significant results are achieved by spectroscopic methods, mainly Raman and infrared. It is noteworthy that spectroscopic methods using microscopy assist in the accuracy of the analyzed region of the fingerprint, contributing to more robust results. There was also a significant increase in studies using methods focused on finding new developers or identifying components present in fingerprints by rapid tests. This systematic review of analytical techniques applied to the detection of fingerprints explores different approaches to contribute to future studies in forensic identification, verifying new demands in the forensic sciences and assisting in the selection of studies for the progress of research.

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confidence: 99%

Methodologies Applied to Fingerprint Analysis

González

Gorziza

Mariotti

et al. 2020

Journal of Forensic Sciences

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“…Ainda que um xenobiótico exerça sua ação tóxica em um órgão ou tecido específico (que geralmente é o foco principal dos estudos toxicológicos), deve-se considerar também sua ação secundária no indivíduo intoxicado. A metabolômica tem sido aplicada na toxicologia forense e trabalhos nessa área têm sido revisados frequentemente, [258][259][260][261][262] mostrando que o estudo comparativo do perfil metabólico pode desempenhar um papel importante na descoberta e validação de novos biomarcadores, além de contribuir para o entendimento e consequente interpretação dos mecanismos de ação tóxica de xenobióticos. 263 A dependência de drogas como cocaína, anfetaminas, opióides e etanol é um grave problema de saúde pública e, recentemente, estudos de metabolômica vem sendo conduzidos para [264][265][266][267][268] na busca por biomarcadores para hepatotoxicidade e nefrotoxicidade induzida pela ação de drogas 269,270 e, ainda, métodos de quantificação de drogas e seus metabólitos vêm sendo desenvolvidos para aplicação em diversas matrizes biológicas.…”

Section: Toxicologia Forenseunclassified

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

Canuto¹,

Costa²,

Cruz³

et al. 2017

Quim. Nova

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METABOLOMICS: DEFINITIONS, STATE-OF-THE-ART AND REPRESENTATIVE APPLICATIONS.Metabolomics is an emerging and promising omics approach used to understand biological mechanisms. By untargeted and targeted metabolomics analyses, metabolites are determined in biological samples (fluids, cells, tissues, etc.) by comparison of control groups with altered groups, undergoing different therapies, submitted to differing stress levels, dietary modulation, or promoted by a disease, or specific condition, etc., using sophisticated analytical techniques, and advanced data treatment and statistical analyses. In this review, the concepts involved in metabolomics studies were presented, describing in details all steps involved in the metabolomics workflow, for untargeted and targeted strategies. Finally, the potential of metabolomics is illustrated by applications in representative areas: clinical, environmental, food and nutrition, forensic toxicology, microbiology, parasitology, plants, and sports. Relevant reviews were compiled to characterize each of these areas, and a corresponding application of untargeted and targeted metabolomics were described.Keywords: metabolomics; untargeted metabolomics; targeted metabolomics; metabolomics workflow. INTRODUÇÃOAs ciências ômicas buscam o entendimento do funcionamento celular dos organismos e suas alterações biológicas. Fazem parte deste conjunto de ciências, a genômica (estudo da alteração dos genes), a transcriptômica (estudo das alterações dos transcritos), a proteômica (estudo das alterações das proteínas), e a metabolômica (estudo das alterações dos metabólitos). Metabólitos são produtos intermediários ou finais do metabolismo em uma amostra biológica.1 O conjunto de todos os metabólitos de baixa massa molecular (até 1500 Da), presentes ou alterados em um sistema biológico, é chamado de metaboloma (do inglês, metabolome).2 A pesquisa relacionada a metabólitos vem sendo desenvolvida há décadas, mas em 1999, Nicholson et al. definiram a metabonômica (do inglês, metabonomics), como sendo a medida quantitativa da resposta metabólica de um sistema biológico após estímulos fisiopatológicos ou modificações genéticas.3 Já o termo metabolômica (do inglês, metabolomics) foi introduzido em 2001, por Oliver Fiehn, como sendo a análise abrangente e quantitativa do metaboloma de um sistema biológico. 4 Além disso, a literatura nos apresenta outras denominações envolvendo esse campo da ciência, tais como: perfil metabólico (do inglês, metabolic profiling), descrito como sendo a análise de metabólitos previamente selecionados de rotas bioquímicas específicas, 5 a impressão digital metabólica (do inglês, metabolic fingerprinting), que é definida como sendo "uma classificação de amostras de acordo com sua origem ou sua relevância biológica", 3 e, por último, análise footprinting (ainda sem tradução para o português), para se referir aos metabólitos excretados por uma célula em condições controladas. Observa-se, portanto, uma divergência em relação às terminologias aplicadas às análises metabolômic...

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“…At present, electrochemical biosensors have been reported for the detection and quantification of most target compounds of interest in forensic analysis. However, despite their advantages, the real applications of biosensors in this field are still scarce [6], and, in several occasions, the biosensors have not been validated for the analysis of complex samples. Electrochemical biosensors exhibit advantageous features inherent to the electrochemical detection, such as high sensitivity, great precision and accuracy, easy handling, low cost, minimal sample requirement, simple integration into portable platforms, low power consumption, and multiplexing capabilities [7], which make them extremely attractive in forensic analysis.…”

Section: Introductionmentioning

confidence: 99%

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

et al. 2019

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This article critically discusses the latest advances in the use of voltammetric, amperometric, potentiometric, and impedimetric biosensors for forensic analysis. Highlighted examples that show the advantages of these tools to develop methods capable of detecting very small concentrations of analytes and provide selective determinations through analytical responses, without significant interferences from other components of the samples, are presented and discussed, thus stressing the great versatility and utility of electrochemical biosensors in this growing research field. To illustrate this, the determination of substances with forensic relevance by using electrochemical biosensors reported in the last five years (2015–2019) are reviewed. The different configurations of enzyme or affinity biosensors used to solve analytical problems related to forensic practice, with special attention to applications in complex samples, are considered. Main prospects, challenges to focus, such as the fabrication of devices for rapid analysis of target analytes directly on-site at the crime scene, or their widespread use and successful applications to complex samples of interest in forensic analysis, and future efforts, are also briefly discussed.

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An overview on forensic analysis devoted to analytical chemists

Cited by 40 publications

References 97 publications

Methodologies Applied to Fingerprint Analysis

Methodologies Applied to Fingerprint Analysis

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

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