An overview on fermentation strategies to overcome lignocellulosic inhibitors in second-generation ethanol production using cell immobilization

Soares, Lauren B.; Silveira, Jussara María; Biazi, Luiz Eduardo; Longo, Liana; Cansian, Rogério Luís; Fúrigo, Agenor; Ienczak, Jaciane Lutz

doi:10.1080/07388551.2022.2109452

Cited by 8 publications

(7 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The global volumetric ethanol productivity found herein (1.59 g L −1 h −1 ) was lower than the values achieved by Milessi, Silva, et al (2020) (5.7 g L −1 h −1 , 2 G process), Milessi, Zangirolami, et al (2020) (4.4 g L −1 h −1 , 2G process) and Perez et al (2022) (14.06–22.80 g L −1 h −1 , fixed‐bed bioreactor with 20 repeated batches, 1G2G process) that used immobilized cells of S. cerevisiae modified to consume xylose. Cell immobilization has been used to overcome the interference of inhibitors in the production of 2 G ethanol and to attain high global volumetric productivities, and this theme was recently the focus of an overview (Soares et al, 2022). However, it is important to emphasize that the addition of an immobilization step could increase both the costs with inputs, as well as with processes and energy, and this could be a disadvantage about this technique (Soares et al, 2022).…”

Section: Resultsmentioning

confidence: 99%

“…Cell immobilization has been used to overcome the interference of inhibitors in the production of 2 G ethanol and to attain high global volumetric productivities, and this theme was recently the focus of an overview (Soares et al, 2022). However, it is important to emphasize that the addition of an immobilization step could increase both the costs with inputs, as well as with processes and energy, and this could be a disadvantage about this technique (Soares et al, 2022). On the other hand, productivity was higher than that observed by Novy et al (2017) that used free cells of S. cerevisiae genetically modified in a 2G process (0.58 g L −1 h −1 ).…”

Section: Resultsmentioning

confidence: 99%

Section: Propagation and Fed-batch Fermentation By S Cerevisiae Mp-c5h1mentioning

confidence: 99%

“…After adsorption, the HMF and furfural concentrations were also reduced by 53% and 90%, respectively. It has been widely studied that weak acids affect the membrane potential and integrity of yeasts (Freitas et al, 2013;Soares et al, 2020Soares et al, , 2022Zhao & Bai, 2009) during the fermentation step. Furfural, 5-HMF, organic acids, and phenolic compounds can directly affect cell metabolism during fermentation, decreasing the efficiency and productivity of ethanol (Lima et al, 2021;Soares et al, 2020).…”

Section: Kinetic Parametersmentioning

confidence: 99%

See 3 more Smart Citations

First‐ and second‐generation integrated process for bioethanol production: Fermentation of molasses diluted with hemicellulose hydrolysate by recombinant Saccharomyces cerevisiae

de Oliveira Pereira,

dos Santos,

Guimarães

et al. 2024

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

The integration of first‐ (1G) and second‐generation (2G) ethanol production by adding sugarcane juice or molasses to lignocellulosic hydrolysates offers the possibility to overcome the problem of inhibitors (acetic acid, furfural, hydroxymethylfurfural and phenolic compounds), and add nutrients (such as salts, sugars and nitrogen sources) to the fermentation medium, allowing the production of higher ethanol titers. In this work, an 1G2G production process was developed with hemicellulosic hydrolysate (HH) from a diluted sulfuric acid pretreatment of sugarcane bagasse and sugarcane molasses. The industrial Saccharomyces cerevisiae CAT‐1 was genetically modified for xylose consumption and used for co‐fermentation of sucrose, fructose, glucose, and xylose. The fed‐batch fermentation with high cell density that mimics an industrial fermentation was performed at bench scale fermenter, achieved high volumetric ethanol productivity of 1.59 g L−1 h−1, 0.39 g g−1 of ethanol yield, and 44.5 g L−1 ethanol titer, and shown that the yeast was able to consume all the sugars present in must simultaneously. With the results, it was possible to establish a mass balance for the global process: from pretreatment to the co‐fermentation of molasses and HH, and it was possible to establish an effective integrated process (1G2G) with sugarcane molasses and HH co‐fermentation employing a recombinant yeast.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Propagation and Fed-batch Fermentation By S Cerevisiae Mp-c5h1mentioning

confidence: 99%

Section: Kinetic Parametersmentioning

confidence: 99%

See 2 more Smart Citations

First‐ and second‐generation integrated process for bioethanol production: Fermentation of molasses diluted with hemicellulose hydrolysate by recombinant Saccharomyces cerevisiae

de Oliveira Pereira,

dos Santos,

Guimarães

et al. 2024

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

show abstract

“…A carrier should be nontoxic, biodegradable, and cost-effective. For yeasts cells, mainly Ca-alginate, carrageenan, cellulose, chitosan, silica-hydrogel, and pre-polymers are used as carriers [12,136,[148][149][150][151][152].…”

Section: Ethanol Fermentationmentioning

confidence: 99%

Bioethanol Production from Lignocellulosic Biomass—Challenges and Solutions

2022

View full text Add to dashboard Cite

Regarding the limited resources for fossil fuels and increasing global energy demands, greenhouse gas emissions, and climate change, there is a need to find alternative energy sources that are sustainable, environmentally friendly, renewable, and economically viable. In the last several decades, interest in second-generation bioethanol production from non-food lignocellulosic biomass in the form of organic residues rapidly increased because of its abundance, renewability, and low cost. Bioethanol production fits into the strategy of a circular economy and zero waste plans, and using ethanol as an alternative fuel gives the world economy a chance to become independent of the petrochemical industry, providing energy security and environmental safety. However, the conversion of biomass into ethanol is a challenging and multi-stage process because of the variation in the biochemical composition of biomass and the recalcitrance of lignin, the aromatic component of lignocellulose. Therefore, the commercial production of cellulosic ethanol has not yet become well-received commercially, being hampered by high research and production costs, and substantial effort is needed to make it more widespread and profitable. This review summarises the state of the art in bioethanol production from lignocellulosic biomass, highlights the most challenging steps of the process, including pretreatment stages required to fragment biomass components and further enzymatic hydrolysis and fermentation, presents the most recent technological advances to overcome the challenges and high costs, and discusses future perspectives of second-generation biorefineries.

show abstract

Design a reducing CO2 emission system using nonfermentable substrates for carbon-economic biosynthesis of poly-2-hydrobutanedioic acid

He,

Zuo,

Chen

et al. 2024

Chemical Engineering Journal

View full text Add to dashboard Cite

An overview on fermentation strategies to overcome lignocellulosic inhibitors in second-generation ethanol production using cell immobilization

Cited by 8 publications

References 75 publications

First‐ and second‐generation integrated process for bioethanol production: Fermentation of molasses diluted with hemicellulose hydrolysate by recombinant Saccharomyces cerevisiae

First‐ and second‐generation integrated process for bioethanol production: Fermentation of molasses diluted with hemicellulose hydrolysate by recombinant Saccharomyces cerevisiae

Bioethanol Production from Lignocellulosic Biomass—Challenges and Solutions

Design a reducing CO2 emission system using nonfermentable substrates for carbon-economic biosynthesis of poly-2-hydrobutanedioic acid

Contact Info

Product

Resources

About