An overview on different strategies for the stemness maintenance of MSCs

Arezoumand, Khatereh Saei; Alizadeh, Effat; Pilehvar-Soltanahmadi, Younes; Esmaeillou, Mohammad; Zarghami, Nosratollah

doi:10.1080/21691401.2016.1246452

Cited by 54 publications

(36 citation statements)

References 120 publications

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“…These results demonstrate that EO-PCL/PEG nanofibres have high biocompatibility compared with PCL/PEG nanofibres. Also, using co-polymerized PCL with PEG has provided highly biocompatible, hydrophilic, non-toxic, non-immunogenic, and non-antigenic nanofibres [22,23]. The high viabilities of macrophages on EO-PCL/PEG nanofibres partially reflects biocompatibility of the composite biomaterials and can be benefit for wound healing process and implant survival.…”

Section: Macrophage Viabilitymentioning

confidence: 99%

Macrophage repolarization using emu oil-based electrospun nanofibers: possible application in regenerative medicine

Zamani

Pilehvar-Soltanahmadi

Zarghami

2017

Artificial Cells, Nanomedicine, and Biotechnology

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In the regenerative medicine therapies, the availability of engineered scaffolds that modulate inflammatory states is highly required. The aim of this study was to evaluate the efficiency of electrospun nanofibrous scaffolds containing natural substances with anti-inflammatory properties such as Emu oil (EO) to control inflammation and re-polarization of macrophages toward M2 anti-inflammatory phonotype. For this purpose, bead free and smooth EO-blended PCL/PEG electrospun nanofibrous mats were successfully fabricated and characterized using FE-SEM, FTIR, and Universal Testing Machine. GC/MS findings of pure EO revealed the fatty acids composition. MTT results showed that macrophage viability on EO-PCL/PEG nanofibres was higher than on PCL/PEG nanofibres and control (p ≤ .05). Additionally, the presence of EO into nanofibres was found to influence on macrophage morphologies, using FE-SEM. qPCR results showed a reduction in iNOS-2 and an increase in Arg-1 levels of macrophages seeded on EO-PCL/PEG nanofibres, indicating the successfully polarization of the macrophages to M2 phenotype. The change in macrophage phenotype on EO-based nanofibres could suppress the inflammation in LPS/IFN-γ stimulated macrophages as evidenced by a major reduction in pro-inflammatory cytokine levels TNF-α, IL-1β, and IL-6. Conclusively, the results demonstrated that EO-based nanofibres efficiently modulated RAW264.7 macrophage polarity toward an anti-inflammatory M2 phenotype.

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Section: Macrophage Viabilitymentioning

confidence: 99%

Macrophage repolarization using emu oil-based electrospun nanofibers: possible application in regenerative medicine

Zamani

Pilehvar-Soltanahmadi

Zarghami

2017

Artificial Cells, Nanomedicine, and Biotechnology

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“…Type I diabetes is caused by the autoimmune destruction of beta cells in pancreatic islets. While replacing pancreatic beta cells would be an ideal therapy [24,25], generation of beta cells from stem or other cells is an attractive alternative [26][27][28]. In pancreatic islets, only a small subset of cells expresses nestin, and these have been proposed to be precursors of differentiated pancreatic endocrine cells [13].…”

Section: Discussionmentioning

confidence: 99%

Melatonin promotes self-renewal of nestin-positive pancreatic stem cells through activation of the MT2/ERK/SMAD/nestin axis

Bai

Gao

Zhang

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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Although melatonin has been shown to exhibit a wide variety of biological functions, its effects on promotion of self-renewal in pancreatic stem cells remain unknown. In this study, we incubated murine pancreatic stem cells (PSCs) with various concentrations of melatonin (0.01, 0.1, 1, 10 or 100 μM) to screen for the optimum culture medium for increasing cell proliferation. We found that 10 μM melatonin can significantly increase proliferation and enhance expression of a stem cell marker, nestin, in PSCs via melatonin receptor 2 (MT2). Thus, we used 10 μM melatonin to study the melatonin-mediated molecular mechanisms of cell proliferation in PSCs. We applied extracellular signal-regulated kinase (ERK) pathway inhibitor SCH772984 and transforming growth factor beta (TGF-β) pathway inhibitor SB431542, along with interfering RNAs siERK1, siERK2, siSmad2, siSmad3, siSmad4 and siNestin, to melatonin-treated PSCs to research the roles of these genes in self-renewal. The results revealed a novel molecular mechanism by which melatonin promotes self-renewal of PSCs: a chain reaction in the MT2/ERK/SMAD/nestin axis promoted the aforementioned self-renewal as well as inhibited differentiation. In addition, upregulation of nestin created a positive feedback loop in the regulation of the transforming growth factor beta 1 (TGF-β1)/SMADs pathway by promoting expression of Smad4. Conversely, knockdown of nestin significantly suppressed the proliferative effect in melatonin-treated PSCs. These are all novel mechanisms through which the ERK pathway cooperatively crosstalks with the SMAD pathway to regulate nestin expression, thereby enhancing self-renewal in PSCs.

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“…Regarding the self‐renewal activity and plasticity extension of stem cells to several lineages, the elucidation of both in vivo and in vitro factors controlling multipotential properties is required to improve the basic knowledge of stem cells biology for providing large enough quantity of cells and further therapeutic manipulation (Saei Arezoumand et al ., ; Dadashpour et al ., ).…”

Section: Stem Cells Characteristics At a Glancementioning

confidence: 99%

Emerging Importance of Phytochemicals in Regulation of Stem Cells Fate via Signaling Pathways

Dadashpour

Pilehvar-Soltanahmadi

Firouzi-Amandi

et al. 2017

Phytotherapy Research

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To reach ideal therapeutic potential of stem cells for regenerative medicine purposes, it is essential to retain their self-renewal and differentiation capacities. Currently, biological factors are extensively used for stemness maintaining and differentiation induction of stem cells. However, low stability, high cost, complicated production process, and risks associated with viral/endotoxin infection hamper the widespread use of biological factors in the stem cell biology. Moreover, regarding the modulation of several signaling cascades, which lead to a distinct fate, phytochemicals are preferable in the stem cells biology because of their efficiency. Considering the issues related to the application of biological factors and potential advantages of phytochemicals in stem cell engineering, there is a considerable increasing trend in studies associated with the application of novel alternative molecules in the stem cell biology. In support of this statement, we aimed to highlight the various effects of phytochemicals on signaling cascades involved in commitment of stem cells. Hence, in this review, the current trends in the phytochemicals-based modulation of stem cell fate have been addressed. Copyright © 2017 John Wiley & Sons, Ltd.

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An overview on different strategies for the stemness maintenance of MSCs

Cited by 54 publications

References 120 publications

Macrophage repolarization using emu oil-based electrospun nanofibers: possible application in regenerative medicine

Macrophage repolarization using emu oil-based electrospun nanofibers: possible application in regenerative medicine

Melatonin promotes self-renewal of nestin-positive pancreatic stem cells through activation of the MT2/ERK/SMAD/nestin axis

Emerging Importance of Phytochemicals in Regulation of Stem Cells Fate via Signaling Pathways

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