An overview on biological production of functional lactose derivatives

Xiao, Yaqin; Chen, Qiuming; Guang, Cuie; Zhang, Wenli; Mu, Wanmeng

doi:10.1007/s00253-019-09755-6

Cited by 42 publications

(24 citation statements)

References 66 publications

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“…One is to transfer the β-galactosyl group produced by the decomposition of lactose to the C4 hydroxyl group of glucosyl in sucrose by the catalysis of β-galactosidase (EC 3.2.1.23); The other is to transfer the fructose group produced by the decomposition of sucrose to the C1 hydroxyl group at the reducing end of the lactose by the catalysis of βfructofuranosidase (EC 3.2.1.26) or levansucrase (EC 2.4.1.10). The use of β-galactosidase for lactosucrose production may cause the formation of the other oligosaccharide by-products such as galactooligosaccharide during the transgalactosylation [9], which makes the purification process more difficult. βfructofuranosidase and levansucrase are classified into the glycoside hydrolase family 68 (GH68) in the CAZy database.…”

Section: Introductionmentioning

confidence: 99%

Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138

Chen

Deng

et al. 2020

Bioengineered

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As a prebiotics, lactosucrose plays an important role in maintaining human gastrointestinal homeostasis. In this study, a thermostable enzyme from Arthrobacter sp. 10138 was screened from six βfructofuranosidase-producing strains for the lactosucrose production and the coding gene was heterologously expressed in Escherichia coli for efficient expression. Recombinant βfructofuranosidase was purified and biochemically characterized by MALDI-TOFMS spectrometry. The transfructosylation product by this recombinant enzyme was determined to be lactosucrose rather than other oligosaccharides or polysaccharides by HPLC and LC-MS. Efficient extracellular secretion of β-fructofuranosidase was achieved by the optimization of signal peptide and induction conditions. It was found that with the signal peptide torT, the highest extracellular activity reached 111.01 U/mL, which was 38.4-fold higher than that with the OmpA signal peptide. Under the optimal conditions (pH 6.0, temperature 50°C, enzyme amount 40 μg/ml, sucrose 150 g/L and lactose 150 g/L), 109 g/L lactosucrose was produced with a molar conversion ratio of 49.3%. Here the thermostable βfructofuranosidase from Arthrobacter sp. 10138 can be used for efficient synthesis of lactosucrose, and this provides a good startpoint for the industrial production of lactosucrose in the future.

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Section: Introductionmentioning

confidence: 99%

Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138

Chen

Deng

et al. 2020

Bioengineered

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“…Dried fruits (e.g., nuts) contain PUFAs, but oily fish, algae, and molluscs are the richest dietary sources of these molecules [ 55 , 56 ]. For its part, the identification of lactose, occurring in milk and its derivatives, probably testified to the intake of dairy products [ 57 ].…”

Section: Resultsmentioning

confidence: 99%

Investigating Plant Micro-Remains Embedded in Dental Calculus of the Phoenician Inhabitants of Motya (Sicily, Italy)

D’Agostino

Canini

Marco

et al. 2020

Plants

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Plant records reveal remarkable evidence about past environments and human cultures. Exploiting dental calculus analysis and using a combined approach of microscopy and gas chromatography mass spectrometry, our research outlines dietary ecology and phytomedicinal practices of the ancient community of Motya (Sicily, eight to sixth century BC), one of the most important Phoenician settlements in the Mediterranean basin. Micro-remains suggest use or consumption of Triticeae cereals, and animal-derived sources (e.g., milk and aquatic birds). Markers of grape (or wine), herbs, and rhizomes, endemic of Mediterranean latitudes and the East, provide insight into the subsistence of this colony, in terms of foodstuffs and phytotherapeutic products. The application of resins and wood of Gymnosperms for social and cultural purposes is hypothesized through the identification of Pinaceae secondary metabolites and pollen grains. The information hidden in dental calculus discloses the strong human-plant interaction in Motya’s Phoenician community, in terms of cultural traditions and land use.

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“…The well-known procedure developed by Holler, as explained in (Fox et al 2004 ; Xiao et al 2019 ) was used to recover protein from the milk of Indian Gir cow, and from that available in the local supermarket. Fresh, undiluted milk, the fat of which was removed by centrifugation at 4000 rpm, was cooled to 10 °C, and was used for further processing.…”

Section: Methodsmentioning

confidence: 99%

Favourable Interfacial Characteristics of A2 Milk Protein Monolayer

Dhopte

Lad

2022

J Membrane Biol

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Shielding of the specific body organ using the biocompatible material helps preventing direct exposure of that part to the foreign entities responsible for infections. Here we show the potential of the A2 milk protein recovered from the milk of cow from Indian origin for possible prevention of the direct exposure to other foreign molecules. We measured the surface pressure of the monolayers of different types of protein samples using Langmuir isotherm experiments. The surface pressure measurements for the monolayer of four types of protein macromolecules have been carried out using the Wilhelmy plate micro pressure sensor. We studied the self-organization of different protein macromolecules and their monolayer compression characteristics. The electrochemical behaviour is studied using electrochemical impedance spectroscopy. We found the highest surface pressure for the monolayer of A2 protein. Further, it is also found that A2 protein exhibited the highest surface activity amongst the other proteins. This property can be effectively used for making the envelope of the A2 protein surrounding the targeted entity. Graphical Abstract

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An overview on biological production of functional lactose derivatives

Cited by 42 publications

References 66 publications

Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138

Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138

Investigating Plant Micro-Remains Embedded in Dental Calculus of the Phoenician Inhabitants of Motya (Sicily, Italy)

Favourable Interfacial Characteristics of A2 Milk Protein Monolayer

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