An Overview of Zebrafish Modeling Methods in Drug Discovery and Development

Larijani, Bagher; Hamidpour, Shayesteh Kokabi; Tayanloo-Beik, Akram; Shahbazbadr, Ainaz; Yavari, Hanieh; Namazi, Nazli; Biglar, Mahmood; Arjmand, Babak

doi:10.1007/5584_2021_684

Cited by 8 publications

(7 citation statements)

References 152 publications

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“…However, the use of animal models in preclinical studies is still controversial. Because the use of animals in scientific studies requires the observance of strict ethical principles (72,(182)(183)(184). Thus, in recent years, studies in the field of neurological disorders have led to the use of new technologies such as organ-on-a-chip.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, in recent years, studies in the field of neurological disorders have led to the use of new technologies such as organ-on-a-chip. Since this strategy is based on the use of the patient's stem cells, some of the issues accompanied by applying animal models during the drug discovery and development platforms can be almost addressed and the studies can be upgraded to a more accurate level (183). Moreover, the application of new technologies such as artificial intelligence (AI) and its subsets (e.g., machine learning) as cutting-edge technologies could revolutionize biomedical studies, particularly ASD diagnosis and treatment (185)(186)(187).…”

Section: Discussionmentioning

confidence: 99%

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Zebrafish Modeling of Autism Spectrum Disorders, Current Status and Future Prospective

et al. 2022

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Autism spectrum disorder (ASD) refers to a complicated range of childhood neurodevelopmental disorders which can occur via genetic or non-genetic factors. Clinically, ASD is associated with problems in relationships, social interactions, and behaviors that pose many challenges for children with ASD and their families. Due to the complexity, heterogeneity, and association of symptoms with some neuropsychiatric disorders such as ADHD, anxiety, and sleep disorders, clinical trials have not yielded reliable results and there still remain challenges in drug discovery and development pipeline for ASD patients. One of the main steps in promoting lead compounds to the suitable drug for commercialization is preclinical animal testing, in which the efficacy and toxicity of candidate drugs are examined in vivo. In recent years, zebrafish have been able to attract the attention of many researchers in the field of neurological disorders such as ASD due to their outstanding features. The presence of orthologous genes for ASD modeling, the anatomical similarities of parts of the brain, and similar neurotransmitter systems between zebrafish and humans are some of the main reasons why scientists draw attention to zebrafish as a prominent animal model in preclinical studies to discover highly effective treatment approaches for the ASD through genetic and non-genetic modeling methods.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Zebrafish Modeling of Autism Spectrum Disorders, Current Status and Future Prospective

et al. 2022

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“…A zebrafish larvae model was established for in vivo studies of diabetes mellitus with heart failure with reduced ejection fraction (DM‐HFrEF) by sequentially treating them with a combination of d ‐glucose (Kim et al., 2023), STZ and terfenadine, a potassium channel blocker. Diabetic zebrafish treated with terfenadine showed significantly less myocardial fractional shortening, more irregular contractions, significant increase in the levels of natriuretic peptide B (a heart failure biomarker), markedly reduced motility and reduced survival rates (Larijani et al., 2021; Kim et al., 2023). The importance of this novel model relies in the possibility of performing mechanistic studies to understand the pathophysiology of DM‐HFrEF (Kim et al., 2023).…”

Section: Zebrafish Models For Diabetes and Diabetic Pathologiesmentioning

confidence: 99%

“…For instance, aflibercept 100 μg/mL efficacy was confirmed, as it could improve the hyperglycaemia‐increased expression of diabetic retinopathy inflammatory markers and the diameters of retinal vessels in the zebrafish eye. Moreover, the pdx1 mutant is a valuable resource for molecular studies to identify new targets for the treatment of early as well as late diabetic retinopathy (Larijani et al., 2021). In the pdx1 −/− zebrafish mutant DR model, both vascular aspects responded to antiangiogenic and antihyperglycaemic pharmacological interventions in the larval stage, and were accompanied by alterations in nitric oxide metabolism.…”

Section: Drugs For Diabetic Pathologiesmentioning

confidence: 99%

Beyond drug discovery: Exploring the physiological and methodological dimensions of zebrafish in diabetes research

Roohi,

Faizan,

Shaikh

et al. 2024

Experimental Physiology

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Diabetes mellitus is a chronic disease that is now considered a global epidemic. Chronic diabetes conditions include type 1 and type 2 diabetes, both of which are normally irreversible. As a result of long‐term uncontrolled high levels of glucose, diabetes can progress to hyperglycaemic pathologies, such as cardiovascular diseases, retinopathy, nephropathy and neuropathy, among many other complications. The complete mechanism underlying diabetes remains unclear due to its complexity. In this scenario, zebrafish (Danio rerio) have arisen as a versatile and promising animal model due to their good reproducibility, simplicity, and time‐ and cost‐effectiveness. The Zebrafish model allows us to make progress in the investigation and comprehension of the root cause of diabetes, which in turn would aid in the development of pharmacological and surgical approaches for its management. The current review provides valuable reference information on zebrafish models, from the first zebrafish diabetes models using genetic, disease induction and chemical approaches, to the newest ones that further allow for drug screening and testing. This review aims to update our knowledge related to diabetes mellitus by gathering the most authoritative studies on zebrafish as a chemical, dietary and insulin induction, and genetic model for diabetes research.

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“…Zebrafish also possess a high fecundity, allowing for genetic screens [ 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 , 143 , 144 ] and high throughput assays to assess varied compounds and their role in developmental processes [ 145 , 146 , 147 , 148 , 149 , 150 , 151 , 152 ]. For example, RAR and RXR agonists/antagonists and their role in developmental processes can be explored by adding these compounds at various times ( Figure 2 ).…”

Section: Ra and Zebrafish A Backgroundmentioning

confidence: 99%

Zebrafish as a Model to Study Retinoic Acid Signaling in Development and Disease

Hawkins

Wingert

2023

Biomedicines

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Retinoic acid (RA) is a metabolite of vitamin A (retinol) that plays various roles in development to influence differentiation, patterning, and organogenesis. RA also serves as a crucial homeostatic regulator in adult tissues. The role of RA and its associated pathways are well conserved from zebrafish to humans in both development and disease. This makes the zebrafish a natural model for further interrogation into the functions of RA and RA-associated maladies for the sake of basic research, as well as human health. In this review, we explore both foundational and recent studies using zebrafish as a translational model for investigating RA from the molecular to the organismal scale.

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An Overview of Zebrafish Modeling Methods in Drug Discovery and Development

Cited by 8 publications

References 152 publications

Zebrafish Modeling of Autism Spectrum Disorders, Current Status and Future Prospective

Zebrafish Modeling of Autism Spectrum Disorders, Current Status and Future Prospective

Beyond drug discovery: Exploring the physiological and methodological dimensions of zebrafish in diabetes research

Zebrafish as a Model to Study Retinoic Acid Signaling in Development and Disease

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