An Overview of Virulence-Associated Factors of Gram-Negative Fish Pathogenic Bacteria

Méndez, Jessica; Reimundo, Pilar; Pérez-Pascual, David; Navais, Roberto; Gómez, Esther Romero; Cascales, Desirée; Guijarro, José A.

doi:10.5772/29837

Cited by 11 publications

(9 citation statements)

References 127 publications

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“…We indeed found several proteases, lipases, phospholipases, nucleases, haemolysins and murein hydrolases associated to OMVs, as revealed by MS‐MS sequencing. Together with siderophore receptors and adhesins/invasins, also found associated to LGP32 OMVs (Supporting Information http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12535/suppinfo), these hydrolases correspond to potential virulence factors described in pathogenic Vibrio species (Zhang and Austin, ; Méndez et al ., ). Their association with OMVs is in agreement with the protease, phospholipase and haemolysin activities associated to OMVs of V. anguillarum , another pathogen for marine cultured species (Hong et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Two putative haemolysins and a phospholipase D (PLD) found in our proteomic data (Supporting Information Table S1) are of particular interest. These toxins are indeed known for their lytic properties on biological membranes, causing damage to erythrocytes and other cell types, such as leukocytes or neutrophils (for review, see Méndez et al, 2012). PLD are involved in virulence of different bacteria.…”

Section: Discussionmentioning

confidence: 99%

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Outer membrane vesicles are vehicles for the delivery ofVibrio tasmaniensisvirulence factors to oyster immune cells

Vanhove

Duperthuy

Charrière

et al. 2014

Environmental Microbiology

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Vibrio tasmaniensis LGP32, a facultative intracellular pathogen of oyster haemocytes, was shown here to release outer membrane vesicles (OMVs) both in the extracellular milieu and inside haemocytes. Intracellular release of OMVs occurred inside phagosomes of intact haemocytes having phagocytosed few vibrios as well as in damaged haemocytes containing large vacuoles heavily loaded with LGP32. The OMV proteome of LGP32 was shown to be rich in hydrolases (25%) including potential virulence factors such as proteases, lipases, phospholipases, haemolysins and nucleases. One major caseinase/gelatinase named Vsp for vesicular serine protease was found to be specifically secreted through OMVs in which it is enclosed. Vsp was shown to participate in the virulence phenotype of LGP32 in oyster experimental infections. Finally, OMVs were highly protective against antimicrobial peptides, increasing the minimal inhibitory concentration of polymyxin B by 16-fold. Protection was conferred by OMV titration of polymyxin B but did not depend on the activity of Vsp or another OMV-associated protease. Altogether, our results show that OMVs contribute to the pathogenesis of LGP32, being able to deliver virulence factors to host immune cells and conferring protection against antimicrobial peptides.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Outer membrane vesicles are vehicles for the delivery ofVibrio tasmaniensisvirulence factors to oyster immune cells

Vanhove

Duperthuy

Charrière

et al. 2014

Environmental Microbiology

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“…Confer & Ayalew, 2012), aerolysins (Howard, Garland, Green, & Buckley, 1987), hemolysins (Asao, Kinoshita, Kozaki, Uemura, & Sakaguchi, 1984), enterotoxins (Chopra, Houston, Peterson, & Jin, 1993;Sha et al, 2005), serine protease (Cascón, Fregeneda, et al, 2000;Méndez et al, 2012), and elastase . Further, ahyR encodes a LuxR-type quorum sensing regulator that regulates the expression of virulence factors in A. hydrophila (Kirke, Swift, Lynch, & Williams, 2004;Swift et al, 1997Swift et al, , 1999.…”

Section: Discussionmentioning

confidence: 99%

“…The pathogenesis of A. hydrophila is multifactorial, and characterized by the involvement of a number of virulence factors, such as adhesins (Fang, Ge, & Sin, ), outer membrane proteins (omps; Confer & Ayalew, ), aerolysins (Howard, Garland, Green, & Buckley, ), hemolysins (Asao, Kinoshita, Kozaki, Uemura, & Sakaguchi, ), enterotoxins (Chopra, Houston, Peterson, & Jin, ; Sha et al., ), serine protease (Cascón, Fregeneda, et al., ; Méndez et al., ), and elastase (Cascón, Yugueros, et al., ). Further, ahyR encodes a LuxR‐type quorum sensing regulator that regulates the expression of virulence factors in A. hydrophila (Kirke, Swift, Lynch, & Williams, ; Swift et al., , ).…”

Section: Discussionmentioning

confidence: 99%

The stress hormone norepinephrine increases the growth and virulence of Aeromonas hydrophila

Gao

Chen

et al. 2018

MicrobiologyOpen

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Stress is an important contributing factor in the outbreak of infectious fish diseases. To comprehensively understand the impact of catecholamine stress hormone norepinephrine (NE) on the pathogenicity of Aeromonas hydrophila, we assessed variations in bacterial growth, virulence-related genes expression and virulence factors activity after NE addition in serum-SAPI medium. Further, we assessed the effects of NE on A. hydrophila virulence in vivo by challenging fish with pathogenic strain AH196 and following with or without NE injection. The NE-associated stimulation of A. hydrophila strain growth was not linear-dose-dependent, and only 100 μM, or higher concentrations, could stimulate growth. Real-time PCR analyses revealed that NE notably changed 13 out of the 16 virulence-associated genes (e.g. ompW, ahp, aha, ela, ahyR, ompA, and fur) expression, which were all significantly upregulated in A. hydrophila AH196 (p < 0.01). NE could enhance the protease activity, but not affect the lipase activity, hemolysis, and motility. Further, the mortality of crucian carp challenged with A. hydrophila AH196 was significantly higher in the group treated with NE (p < 0.01). Collectively, our results showed that NE enhanced the growth and virulence of pathogenic bacterium A. hydrophila.

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“…The ECPs are usually associated with disease symptoms leading to the toxic effects that cause the death of infected fish (Méndez et al. ). They are of low molecular weight and are believed to constitute a lethal toxin generated by V. harveyi (Montero and Austin ).…”

Section: Transmission and Virulence Factors Influencing Vibriosismentioning

confidence: 99%

Vibriosis in Fish: A Review on Disease Development and Prevention

et al. 2018

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Current growth in aquaculture production is parallel with the increasing number of disease outbreaks, which negatively affect the production, profitability, and sustainability of the global aquaculture industry. Vibriosis is among the most common diseases leading to massive mortality of cultured shrimp, fish, and shellfish in Asia. High incidence of vibriosis can occur in hatchery and grow-out facilities, but juveniles are more susceptible to the disease. Various factors, particularly the source of fish, environmental factors (including water quality and farm management), and the virulence factors of Vibrio, influence the occurrence of the disease . Affected fish show weariness, with necrosis of skin and appendages, leading to body malformation, slow growth, internal organ liquefaction, blindness, muscle opacity, and mortality. A combination of control measures, particularly a disease-free source of fish, biosecurity of the farm, improved water quality, and other preventive measures (e.g., vaccination) might be able to control the infection. Although some control measures are expensive and less practical, vaccination is effective, relatively cheap, and easily implemented. In this review, the latest knowledge on the pathogenesis and control of vibriosis, including vaccination, is discussed. 4INA-SALWANY ET AL.

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An Overview of Virulence-Associated Factors of Gram-Negative Fish Pathogenic Bacteria

Cited by 11 publications

References 127 publications

Outer membrane vesicles are vehicles for the delivery ofVibrio tasmaniensisvirulence factors to oyster immune cells

Outer membrane vesicles are vehicles for the delivery ofVibrio tasmaniensisvirulence factors to oyster immune cells

The stress hormone norepinephrine increases the growth and virulence of Aeromonas hydrophila

Vibriosis in Fish: A Review on Disease Development and Prevention

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