An Overview of the Role of Mineral Solubility in Silicosis and Asbestosis

Erdogdu, G.; Hasırcı, Vasıf

doi:10.1006/enrs.1998.3842

Cited by 18 publications

(14 citation statements)

References 28 publications

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“…The interaction between silica particles and cell membrane occurs through hydrogen bonding or free-radicals generation or both (Erdogdu and Hasirci, 1998). The two main surface groups on particles, which determine their biological activity, are siloxane bridges (Si-OSi) and silanols (SiOH).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of cytotoxic concentration–time response in A549 cells exposed to respirable α‐quartz

Fanizza¹,

Fresegna

Maiello

et al. 2009

J of Applied Toxicology

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A causal pathway between quartz, silicosis and lung cancer has been postulated. The aim of our study was to assess cytotoxic effects induced in a human lung epithelial cell line (A549) by exposure to alpha-quartz. Cells were exposed to respirable alpha-quartz (SRM1878a, NIST) at 25, 50 or 100 microg ml(-1 )for 24 h and at 50 or 100 microg ml(-1) for 48 h. Cytotoxic effects were analyzed by scanning electron microscopy (SEM), apoptotic morphology analysis with Hoechst staining and lactate dehydrogenase (LDH) release assay. In cells exposed to alpha-quartz for 24 h, a concentration-dependent bleb development and in particular the localization of blebs at the cell edge at higher concentrations were observed. The blebbing phenomenon was more evident after 48 h of exposure to 50 or to 100 microg ml(-1) of alpha-quartz and large blebs were localized at the cell edge. At the same concentrations surface smoothing was also observed. Moreover the presence of holes and tears was detected at the highest concentration both at 24 and 48 h. Results of morphological analysis with Hoechst stain evidenced an increase concentration-time dependent of apoptotic cell percentage that was more marked after 48 h exposure to 100 microg ml(-1) and a prevalence of late apoptosis stage with the increase of exposure time and concentration. Cells exposed to 50 or 100 microg ml(-1) of alpha-quartz for 24 and 48 h produced a significant increase in LDH release. The concentration-time-dependent bleb induction evidenced by SEM correlates with the increase of apoptotic cells and LDH activity release, demonstrating the onset of cytotoxic effects in human lung cells exposed to alpha-quartz.

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Section: Introductionmentioning

confidence: 99%

Evaluation of cytotoxic concentration–time response in A549 cells exposed to respirable α‐quartz

Fanizza¹,

Fresegna

Maiello

et al. 2009

J of Applied Toxicology

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“…Further studies demonstrate that silica particles could impact on alveolar macrophages that produce specific proteins triggering fibroblast proliferation and therefore formation of fibrous tissues (Arcangeli et al 1990). It was also suggested that not only the particle surface but also its solubilization products could be responsible for toxicity (Erdogu and Hasirci 1998). In parallel, silica particles have long been used in the formulation of oral drugs, although recent concerns have been raised about their capacity to induce sarcoidosis, that was previously observed for crystalline silica particle only (Sola et al 2009).…”

Section: In Vitro Interactions With Cellsmentioning

confidence: 98%

Silica-Based Nanoparticles for Intracellular Drug Delivery

Quignard

Masse

Coradin

2011

Intracellular Delivery

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Silica-based nanoparticles have recently raised a great deal of attention as possible drug carriers. Such an interest is driven by the possibility to control their size, the chemical composition and the porous structure as well as to easily modify their surface with a wide range of biologically-relevant functionalities, favoring colloidal stability, long-time blood circulation and even specific targeting. Drug loading can be performed during particle formation but, at this time, the most popular method relies on the impregnation of pre-formed mesoporous colloids. Strategies to control drug delivery via bio-responsive pore capping are also developed. However, despite an increasing number of in vitro and in vivo studies related to the interaction of silica particles with cells and animals, their biocompatibility is still an issue, especially if applications in intracellular drug delivery are foreseen.

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“…Experimental evidence supporting the influence of these factors has recently been reviewed [Mossman and Churg 1998;Heppleston 1994]. Many in vitro studies have been conducted to investigate the surface characteristics of crystalline silica particles and their influence on fibrogenic activity [Bolsaitis and Wallace 1996;Fubini 1997Fubini , 1998Castranova et al 1996;Donaldson and Borm 1998;Erdogdu and Hasirci 1998]. These researchers found that a number of features may be related to silica cytotoxicity.…”

Section: Silicosis 321 Definitionmentioning

confidence: 99%