An Overview of the Bacterial Carbonic Anhydrases

Supuran, Claudiu T.; Capasso, Clemente

doi:10.3390/metabo7040056

Cited by 183 publications

(199 citation statements)

References 106 publications

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“…The last three classes were recently discovered [12][13][14] . Moreover, members of each class possess multiple transcript variants and protein isoforms, which are characterised by different biochemical properties and have specific tissue/organ and subcellular localizations 11,[15][16][17][18][19][20] . CAs present in animals belong to a-class 21,22 , plants and algae have a-, b-, c-, d-hand i-classes; fungi encode for aand b-CAs; protozoa for a-, band/or g-CAs; bacteria for a-, b-, cand i-CA classes 11,20,[23][24][25][26][27] .…”

Section: Carbonic Anhydrases (Cas)mentioning

confidence: 99%

Carbonic anhydrase IX as a novel candidate in liquid biopsy

Guler

Supuran

Capasso

2019

Journal of Enzyme Inhibition and Medicinal Chemistry

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Among the diagnostic techniques for the identification of tumour biomarkers, the liquid biopsy is considered one that offers future research on precision diagnosis and treatment of tumours in a non-invasive manner. The approach consists of isolating tumor-derived components, such as circulating tumour cells (CTC), tumour cell-free DNA (ctDNA), and extracellular vesicles (EVs), from the patient peripheral blood fluids. These elements constitute a source of genomic and proteomic information for cancer treatment. Within the tumour-derived components of the body fluids, the enzyme indicated with the acronym CA IX and belonging to the superfamily of carbonic anhydrases (CA, EC 4.2.1.1) is a promising aspirant for checking tumours. CA IX is a transmembrane-CA isoform that is strongly overexpressed in many cancers being not much diffused in healthy tissues except the gastrointestinal tract. Here, it is summarised the role of CA IX as tumour-associated protein and its putative relationship in liquid biopsyfor diagnosing and monitoring cancer progression. ARTICLE HISTORY

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Section: Carbonic Anhydrases (Cas)mentioning

confidence: 99%

Carbonic anhydrase IX as a novel candidate in liquid biopsy

Guler

Supuran

Capasso

2019

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…In the aqueous environment of the cell, CO2 can spontaneously hydrate to form bicarbonate, an important substrate for microbial growth (58). The importance of bicarbonate to life is evidenced by the fact that at least 7 different families of carbonic anhydrases (28,32,59) have evolved across all kingdoms of life. These enzymes function to enhance the rate of CO2 hydration to produce bicarbonate.…”

Section: Brucella Cultivation and The Resurrection Of A Carbonic Anhymentioning

confidence: 99%

A carbonic anhydrase pseudogene sensitizes selectBrucellalineages to low CO₂tension

Varesio¹,

Willet

Fiebig

et al. 2019

Preprint

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Brucella are intracellular pathogens that cause a disease known as brucellosis. Though the genus is highly monomorphic at the genetic level, species have animal host preferences and some defining physiologic characteristics. Of note is the requirement for increased CO2 tension to cultivate particular species, which confounded early efforts to isolate B. abortus from diseased cattle. Differences in the capacity of Brucella species to assimilate CO2 are determined by mutations in the carbonic anhydrase gene, bcaA. Ancestral single nucleotide insertions in bcaA have resulted in frameshifted pseudogenes in B. abortus and B. ovis lineages, which underlie their inability to grow under the low CO2 tension of a standard atmosphere. Incubation of wild-type B. ovis in air selects for mutations that "rescue" a functional bcaA reading frame, which enables growth under low CO2 and enhances growth rate in high CO2. Consistent with this result, we show that heterologous expression of functional E. coli carbonic anhydrases enables B. ovis growth in air. Growth of wild-type B. ovis is acutely sensitive to a reduction in CO2 tension, while frame-rescued B. ovis mutants are completely insensitive to CO2 shifts. Wild-type B. ovis initiates a gene expression program upon CO2 downshift that resembles the stringent response and results in activation of its virB type IV secretion system. Our study provides evidence that loss-of-function insertion mutations in bcaA sensitize the response of B. ovis and B. abortus to reduced CO2 tension relative to other Brucella lineages. CO2-dependent starvation and virulence gene expression programs in these species may influence persistence or transmission in natural hosts. ImportanceBrucella spp. are highly related, but exhibit differences in animal host preference that must be determined by genome sequence differences. B. ovis and the majority of B. abortus strains require increased CO2 tension to be cultivated in vitro, and harbor conserved insertional mutations in the carbonic anhydrase, bcaA, which underlie this trait. Mutants that grow in a standard atmosphere, first reported nearly a century ago, are easily selected in the laboratory. These mutants harbor varied indel polymorphisms in bcaA that restore its consensus reading frame and rescue its function. Loss of bcaA function has evolved independently in the B. ovis and B. abortus lineages, and results in a dramatically increased sensitivity to CO2 limitation.

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“…Glaucoma is defined as a multifactorial ophthalmic pathology signalized by optic nerve corruption caused by high intraocular pressure, which can often cause to loss sight. Although several CA inhibitors regulating intraocular pressure such as brinzolamide and dorzolamide are present, their adverse effects restricted the use of these drugs .…”

Section: Introductionmentioning

confidence: 99%

Discovery of Novel Sulfonamide‐Based 5‐Arylidenerhodanines as Effective Carbonic Anhydrase (II) Inhibitors: Microwave‐Assisted and Ultrasound‐Assisted One‐Pot Four‐Component Synthesis, Molecular Docking, and Anti‐CA II Screening Studies

Mermer

Demirbaş

Cakmak

et al. 2019

Journal of Heterocyclic Chem

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The synthesis of N‐substituted‐5‐arylidenerhodanines was carried out by the optimized one‐pot sequential four‐component procedure with the condensation between 4‐aminobenzenesulfonamide, suitable aldehyde, ethyl bromoacetate, and carbon disulfide. In addition to traditional method, microwave‐irradiated and ultrasound‐irradiated techniques were implemented in water at ambitious conditions, and the target compounds were obtained in high yields and purity without purification methods. The enzyme inhibition activity of newly synthesized compounds on carbonic anhydrase (II) was also evaluated. The reference inhibitor molecule was sulfanilamide, the IC50 value of which was 3.5 μM. It was also found that the IC50 values of all examined molecules were in nanomolar level and much smaller than those of sulfanilamide. The inhibition between 93.5 and 99.6% was observed in the presence of new compounds synthesized in the present study at the accessible maximum concentration in the reaction mixtures. 5j, among the tested compounds possessing the lowest IC50 value, was found to be the most potent carbonic anhydrase (II) inhibitor.

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An Overview of the Bacterial Carbonic Anhydrases

Cited by 183 publications

References 106 publications

Carbonic anhydrase IX as a novel candidate in liquid biopsy

Carbonic anhydrase IX as a novel candidate in liquid biopsy

A carbonic anhydrase pseudogene sensitizes selectBrucellalineages to low CO₂tension

Discovery of Novel Sulfonamide‐Based 5‐Arylidenerhodanines as Effective Carbonic Anhydrase (II) Inhibitors: Microwave‐Assisted and Ultrasound‐Assisted One‐Pot Four‐Component Synthesis, Molecular Docking, and Anti‐CA II Screening Studies

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An Overview of the Bacterial Carbonic Anhydrases

Cited by 183 publications

References 106 publications

Carbonic anhydrase IX as a novel candidate in liquid biopsy

Carbonic anhydrase IX as a novel candidate in liquid biopsy

A carbonic anhydrase pseudogene sensitizes selectBrucellalineages to low CO2tension

Discovery of Novel Sulfonamide‐Based 5‐Arylidenerhodanines as Effective Carbonic Anhydrase (II) Inhibitors: Microwave‐Assisted and Ultrasound‐Assisted One‐Pot Four‐Component Synthesis, Molecular Docking, and Anti‐CA II Screening Studies

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A carbonic anhydrase pseudogene sensitizes selectBrucellalineages to low CO₂tension