An overview of recent progress in multiple dual‐mode counter‐current chromatography

Abstract

Counter‐current chromatography is a chromatographic separation and purification technique being developed. The development of different elution modes has significantly contributed to this field. Multiple dual‐mode elution is a method developed based on dual‐mode elution, which consists of a series of changing cycles of the phase role and the direction by switching between normal and reverse elution modes of counter‐current chromatography. This dual‐mode elution method takes full advantage of the liquid nature of stationary and mobile phases of counter‐current chromatography and effectively improves the separation efficiency. So, this unique elution mode has gained extensive attention for separating complex samples. This review mainly describes and summarizes in detail its development, applications, and characteristics in recent years. Meanwhile, its advantages, limitations, and future outlook also have been discussed in this paper.

Efficient discovery of active isolates from Dioscorea spongiosa by the combination of bioassay‐guided macroporous resin column chromatography and high‐speed counter‐current chromatography

“…High-speed counter-current chromatography (HSCCC) is a liquid-liquid partition chromatographic technique, that is capable of avoiding irreversible sample adsorption on a solid support with a great recovery rate, ease scaleup potential, high separation capability, rapid separation, and low solvent use. Thus, this method has become an effective approach to preparatively isolate and purify different natural products [16,17]. Nevertheless, the contents in D. spongiosa are complex, including high, medium, and low polar compounds, and thus the sample pretreatment experiments were necessary for the D. spongiosa extracts to simplify the separation procedures [18,19].…”

Advanced high-resolution chromatographic strategies for efficient isolation of natural products from complex biological matrices: from metabolite profiling to pure chemical entities

“…Instead, the primary objective of this article is to emphasize recent studies that employ cutting-edge, high-resolution chromatographic methods at semi-preparative scales for NP isolation. In this context, chromatographic techniques operating at low resolution (CC, FC, MPLC) or based on liquid partitioning (CPC and HSCCC), although very useful for NP isolation, are not considered in this article and have been well discussed in other recent reviews (de Mello and Leita ¨o, 2023;Gong et al 2020;Li et al 2022;Yang et al 2020;Zhang et al 2023Zhang et al , 2018.…”