An overview of nutritional profiling in foods: Bioanalytical techniques and useful protocols

Abstract

Maintaining a nutritious diet is essential for humans if they want to live a healthier life. Several food businesses and food safety organizations play a significant role and offer useful ways for improving nutritional quality that assists consumers in making informed selections. Making poor food choices and consuming unhealthy meals are the main causes of non-communicable diseases (NCDs). Nutritional profiling (NP) models are developed to evaluate the nutritional value, calorie content, and the amount of micronutrients and macronutrients contained in a given food accompanied by additional details on the nutritional anomaly provided by published standard nutrients and nutritional databases. To construct an ideal nutritional model that can facilitate food consumption, bioanalytical methods such as chromatography, microscopic techniques, molecular assays, and metabolomics can be applied. With the use of these technologies, one can learn more about the health advantages of nutrition and how to prevent disease. A wider element of NP is also provided by the developing technologies in the area of nutrition research, such as nanotechnology, proteomics, and microarray technology. In this review, we are focusing on the different bioanalytical techniques and the various protocols of NP and their application and refinement of the models. We have evaluated various NP techniques currently used in the food industry for the detection of different components present in food items.

Correlative Quantitative Raman Chemical Imaging and MCR–ALS in Mouse NASH Model Reveals Direct Relationships between Diet and Resultant Liver Pathology

“…Links between diet and liver function are usually studied in humans by a range of nonimaging analysis methods, compared with assays of biomarkers for liver function . Diet has been considered in the context of disease, by Raman imaging of metabolic processes, but has not to our knowledge been directly included in the same chemometric model as the target organism.…”

“…Raman spectroscopy is an unlabeled method that can be used as an imaging mode which is particularly sensitive to different fatty acids and can make quantitative comparisons between them, 10 while also being able to visualize other relevant cell/tissue components. 11 Links between diet and liver function are usually studied in humans by a range of nonimaging analysis methods, 12 compared with assays of biomarkers for liver function. 13 Diet has been considered in the context of disease, by Raman imaging of metabolic processes, 14 but has not to our knowledge been directly included in the same chemometric model as the target organism.…”