An overview of nosocomial infections, including the role of the microbiology laboratory.

Emori, T. Grace; Gaynes, Robert P.

doi:10.1128/cmr.6.4.428-442.1993

Cited by 54 publications

(57 citation statements)

References 40 publications

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“…burn injuries, intravenous lines, urinary and dialysis catheters and endotracheal tubes) as well as dysfunctional immune mechanisms (e.g. in neonates, AIDS, cancer and cystic fibrosis patients) (Emori & Gaynes, 1993;Lyczak et al, 2002). Pseudomonas aeruginosa is highly adaptable, forming persistent biofilms and is resistant to many antibiotics.…”

Section: Introductionmentioning

confidence: 99%

The adsorption ofPseudomonas aeruginosabacteriophage ÏKMV is dependent on expression regulation of type IV pili genes

Chibeu

Ceyssens

Hertveldt

et al. 2009

FEMS Microbiology Letters

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Pseudomonas aeruginosa bacteriophage phiKMV requires type IV pili for infection, as observed from the phenotypic characterization and phage adsorption assays on a phage infection-resistant host strain mutant. A cosmid clone library of the host (P. aeruginosa PAO1) genomic DNA was generated and used to select for a clone that was able to restore phiKMV infection in the resistant mutant. This complementing cosmid also re-established type IV pili-dependent twitching motility. The correlation between bacteriophage phiKMV infectivity and type IV pili, along with its associated twitching motility, was confirmed by the resistance of a P. aeruginosa PAO1DeltapilA mutant to the phage. Subcloning of the complementing cosmid and further phage infection analysis and motility assays suggests that a common regulatory mechanism and/or interaction between the ponA and pilMNOPQ gene products are essential for bacteriophage phiKMV infectivity.

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Section: Introductionmentioning

confidence: 99%

The adsorption ofPseudomonas aeruginosabacteriophage ÏKMV is dependent on expression regulation of type IV pili genes

Chibeu

Ceyssens

Hertveldt

et al. 2009

FEMS Microbiology Letters

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“…These traits support its successful colonization of hostile anthropogenic environments where it encounters toxic organic substances, such as detergents and disinfectants, some of which can be utilized as growth substrates. Examples for such environments are industrial wastewaters and healthcare settings where P. aeruginosa is a major cause for hospital infections (Emori and Gaynes, 1993). Sinks for disposal of disinfectants and detergents have been repeatedly identified as environmental sources of several P. aeruginosa outbreaks in clinical settings (Muscarella, 2004).…”

Section: Introductionmentioning

confidence: 99%

Detergent‐induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy

Klebensberger

Lautenschlager

Bressler

et al. 2007

Environmental Microbiology

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During growth of Pseudomonas aeruginosa strain PAO1 with the toxic detergent SDS, a part of the population actively formed macroscopic cell aggregates while the other part grew as freely suspended cells. The physiological function of aggregation for growth with SDS was investigated. Three mutants growing with SDS without aggregation were isolated: the spontaneous mutant strain N and two mutants with transposon insertions in the psl operon for exopolysaccharide synthesis. SDS-induced aggregation in strain N but not in a pslJ mutant was restored by complementation with two genes encoding diguanylate cyclases responsible for synthesis of cyclic-di-guanosine monophosphate (c-di-GMP). By expressing a c-di-GMP-specific phosphodiesterase SDS-induced aggregation of strain PAO1 was reduced. Upon exposure to SDS in the presence of the uncoupler carbonyl cyanide chlorophenylhydrazone, the aggregating strains had ca. 500-fold higher survival rates than the non-aggregating strains. Co-incubation experiments revealed that strain N could integrate into aggregates of strain PAO1 and thereby increase its survival rate more than 1000-fold. These results showed that SDS-induced aggregation involved c-di-GMP signalling with the psl operon as a possible target. Cell aggregation could serve as a pre-adaptive strategy ensuring survival and growth of P. aeruginosa populations in environments with multiple toxic chemicals.

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“…Fluorescent pseudomonads are known for their striking ability to adapt to various ecological niches (Rainey and Travisano, 1998;Spiers et al, 2000). Pseudomonas aeruginosa, a ubiquitous pseudomonad, is also known as a human pathogen causing a variety of severe infections of nosocomial nature (Emori and Gaynes, 1993;Vincent et al, 1995). A classical example of the interplay of P. aeruginosa with its environment is antibiotic resistance.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Pseudomonas aeruginosa oprD gene from clinical and environmental isolates

Pirnay

Vos

Mossialos

et al. 2002

Environmental Microbiology

112

104

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Genomes are constantly evolving. Our report highlights the wide mutational diversity of clinical as well as environmental isolates, compared with the laboratory strain(s), through the systematic genetic analysis of a chromosomal porin gene (oprD) in relation to a specific antibiotic resistance. Mutational inactivation of the oprD gene is associated with carbapenem resistance in Pseudomonas aeruginosa. The sequence of the oprD gene of 55 Pseudomonas aeruginosa natural isolates obtained from across the world--from sources as diverse as patients and rhizospheres--was analysed. A microscale mosaic structure for this gene--resulting from multiple intra- and possibly interspecies recombinational events--is reported. An array of independent and seemingly fast-occurring defective oprD mutations were found, none of which had been described before. A burn wound isolate demonstrated unusually high overall sequence variability typical of mutator strains. We also present evidence for the existence of OprD homologues in other fluorescent pseudomonads.

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An overview of nosocomial infections, including the role of the microbiology laboratory.

Cited by 54 publications

References 40 publications

The adsorption ofPseudomonas aeruginosabacteriophage ÏKMV is dependent on expression regulation of type IV pili genes

The adsorption ofPseudomonas aeruginosabacteriophage ÏKMV is dependent on expression regulation of type IV pili genes

Detergent‐induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy

Analysis of the Pseudomonas aeruginosa oprD gene from clinical and environmental isolates

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An overview of nosocomial infections, including the role of the microbiology laboratory.

Cited by 54 publications

References 40 publications

The adsorption ofPseudomonas aeruginosabacteriophage ÏKMV is dependent on expression regulation of type IV pili genes

The adsorption ofPseudomonas aeruginosabacteriophage ÏKMV is dependent on expression regulation of type IV pili genes

Detergent‐induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy

Analysis of the Pseudomonas aeruginosa oprD gene from clinical and environmental isolates

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The adsorption ofPseudomonas aeruginosabacteriophage ÏKMV is dependent on expression regulation of type IV pili genes

The adsorption ofPseudomonas aeruginosabacteriophage ÏKMV is dependent on expression regulation of type IV pili genes