An Overview of Fractional Exhaled Nitric Oxide and Children with Asthma

Rao, Devika; Phipatanakul, Wanda

doi:10.1586/1744666x.2016.1141049

Cited by 27 publications

(22 citation statements)

References 92 publications

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“…The authors also determined a number of associations in the group of patients with low level of FeNO (<20 ppb): carriage of alleles and genotypes containing short tandem repeats of S (9-11) gene NOS1(AAT)n, with fungal sensitization; carriage of alleles and genotypes containing long tandem repeats of L (12-16) gene NOS2A(CCTTT)n, with reduced FEV1 and FEV1/FVC; carriage of the homozygous genotype of *GG gene ARGII(rs3742879) with epidermal sensitization. With a reduced level of FeNO, the study РОССИЙСКИЙ ВЕСТНИК ПЕРИНАТОЛОГИИ И ПЕДИАТРИИ, 2019; 64: (5) ROSSIYSKIY VESTNIK PERINATOLOGII I PEDIATRII, 2019; 64: (5) ОРИГИНАЛЬНЫЕ СТАТЬИ О пределение уровня оксида азота в выдыхаемом воздухе (FeNO) общепризнано в качестве неинвазивного теста для выявления аллергического воспаления дыхательных путей [1]. Уровень FeNO значительно повышается у детей и взрослых с эозинофильным воспалением дыхательных путей, текущей бронхиальной астмой [1][2][3][4][5].…”

Section: The Article Presents the Results Of A Study Of The Associatiunclassified

“…With a reduced level of FeNO, the study РОССИЙСКИЙ ВЕСТНИК ПЕРИНАТОЛОГИИ И ПЕДИАТРИИ, 2019; 64: (5) ROSSIYSKIY VESTNIK PERINATOLOGII I PEDIATRII, 2019; 64: (5) ОРИГИНАЛЬНЫЕ СТАТЬИ О пределение уровня оксида азота в выдыхаемом воздухе (FeNO) общепризнано в качестве неинвазивного теста для выявления аллергического воспаления дыхательных путей [1]. Уровень FeNO значительно повышается у детей и взрослых с эозинофильным воспалением дыхательных путей, текущей бронхиальной астмой [1][2][3][4][5]. В то же время у ряда пациентов с бронхиальной астмой повышенный уровень FeNO не определяется [1,6].…”

Section: The Article Presents the Results Of A Study Of The Associatiunclassified

“…Индуцибельная NOS (NOS2) индуцируется провоспалительными цитокинами при воспалении дыхательных путей и экспрессируется в основном в макрофагах и эпителиальных клетках [12]. Известно также, что уровень FeNO снижается при применении у больных бронхиальной астмой ИГКС [1][2][3][4][5].…”

Section: Discussionunclassified

“…Больных распределили на 2 группы: генотипы с обеими аллелями коротких (≤14*) повторов (S/S) и генотипы с одним длинным аллелем и с одним коротким повтором (L/S или L/L). Средний уровень FeNO до лечения был (5) значительно выше у больных с генотипами L/S+L/L NOS2(CCTTT)n (154,0±26,8 ppb), чем c генотипами S/S (87,6±9,4 ppb; р=0,019). Выявлена также слабая корреляция между уровнем FeNO до лечения и генотипами L/S+L/L NOS2 (CCTTT)n (r=0,233; p=0,026).…”

Section: Discussionunclassified

“…в группе больных с низким уровнем FeNO (<20 ppb) также определен ряд ассоциаций: носительства аллелей и генотипов, содержащих короткие тандемные повторы S (9-11) гена NOS1(AAT)n с грибковой сенсибилизацией; носительства аллелей и генотипов, содержащих длинные тандемные повторы L (12-16) гена NOS2A(CCTTT)n с пони-РОССИЙСКИЙ ВЕСТНИК ПЕРИНАТОЛОГИИ И ПЕДИАТРИИ, 2019; 64: (5) ROSSIYSKIY VESTNIK PERINATOLOGII I PEDIATRII, 2019; 64: (5) ОРИГИНАЛЬНЫЕ СТАТЬИ женными ОФВ 1 и ОФВ 1 /ФЖЕЛ; носительства гомозиготного генотипа *GG гена ARGII(rs3742879) с эпидермальной сенсибилизацией. При пониженном уровне FeNO определена связь между степенью тяжести бронхиальной астмы и NOS1(AAT)n, степенью эффективности противовоспалительной базисной терапии и NOS1(AAT)n; грибковой сенсибилизацией и NOS1(AAT)n, а также обратная связь между ОФВ 1 и NOS2(CCTTT)n; ОФВ 1 /ФЖЕЛ и NOS2(CCTTT)n.…”

Section: выводыunclassified

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Association of polymorphisms of NO synthases and arginase genes, clinical, laboratory and functional indicators with the level of nitrogen oxide in exhaled air in children with bronchial asthma

Батожаргалова

Дьякова

Петрова

et al. 2019

Ross. vestn. perinatol. pediatr.

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The article presents the results of a study of the associations of NO synthase and arginase genes in children with bronchial asthma with clinical, laboratory and functional parameters depending on the level of nitrogen oxide in exhaled air (FeNO). We examined 107 children with bronchial asthma, they were divided into 2 groups depending on the level of FeNO. We found a number of associations in the group of patients with an elevated level of FeNO (≥20 ppb): carriage of alleles and genotypes containing short tandem repeats of S (9–11) NOS1(AAT)n gene, with an early debut and severe course of the disease, an increased level of total IgE in blood serum; carriage of alleles and genotypes containing long tandem repeats L (12–16) of the NOS2A(CCTTT) n gene, with a moderate course of the disease, with an increased level of IgE; carriage of the allele *A of the ARGII gene (rs3742879) with a moderate course of the disease; carriage of the *G allele and heterozygous *AG genotype of the ARGII gene (rs3742879) with a decreased level of FEV1/FVC; carriage of L alleles and a combination of the SL and LL genotypes of the NOS1(AAT)n gene with elevated blood eosinophils (eosinophilia); a combination of S/L + L/L genotypes of the NOS1(AAT)n gene with fungal sensitization. The authors established the correlations between disease severity and NOS1(AAT)n; the age of the manifestation of the disease and NOS1(AAT)n; FEV1/FVC and ARGII (rs3742879); feedback between blood eosinophilia and NOS1(AAT)n. The authors also determined a number of associations in the group of patients with low level of FeNO (<20 ppb): carriage of alleles and genotypes containing short tandem repeats of S (9–11) gene NOS1(AAT)n, with fungal sensitization; carriage of alleles and genotypes containing long tandem repeats of L (12–16) gene NOS2A(CCTTT)n, with reduced FEV1 and FEV1/FVC; carriage of the homozygous genotype of *GG gene ARGII(rs3742879) with epidermal sensitization. With a reduced level of FeNO, the study determined a relationship between the severity of bronchial asthma and NOS1(AAT)n; degree of effectiveness of anti-inflammatory basic therapy and NOS1(AAT)n; fungal sensitization and NOS1(AAT)n; feedback between FEV1 and NOS2(CCTTT)n; FEV1/FVC and NOS2(CCTTT)n.

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Section: The Article Presents the Results Of A Study Of The Associatiunclassified

Section: Discussionunclassified

Section: выводыunclassified

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Association of polymorphisms of NO synthases and arginase genes, clinical, laboratory and functional indicators with the level of nitrogen oxide in exhaled air in children with bronchial asthma

Батожаргалова

Дьякова

Петрова

et al. 2019

Ross. vestn. perinatol. pediatr.

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Inhibitors of nitric oxide synthase can reduce extracellular traps from neutrophils in asthmatic children in vitro

Wang

Zhu

et al. 2019

Pediatric Pulmonology

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AimThis study aimed to explore the link between neutrophil extracellular traps (NETs) and childhood asthma, to investigate the ability of nitric oxide (NO) to induce NETs in asthmatic children and find inhibitors to reduce NETs in the NO synthesis pathway.MethodsA total of 49 children with mild persistent asthma were included in the study and 20 healthy children's blood samples were collected as healthy controls. Children with asthma were divided into symptomatic and asymptomatic groups according to the presence or absence of symptoms on the day of blood collection. Neutrophils in peripheral blood were isolated and plasma was preserved. NO donor (sodium nitroferricyanide(III) dehydrate [SNP]) could provide NO and proved by a fluorescent probe. A PicoGreen Kit was used to detect the NETs quantificationally. Fluorescence microscopy prepared to observe the main structures of NETs. We measured NETs components (extracellular free double‐stranded DNA [dsDNA]) in healthy, symptomatic and asymptomatic groups' plasma samples, and we compared the ability of SNP with phosphate‐buffered saline, lipopolysaccharides (LPS), and phorbol 12‐myristate 13‐acetate (PMA) to induce NETs. NO synthase (NOS) inhibitors were added to see the impact on NETs formation.ResultsPlasma was obtained from all blood samples of 69 children. The neutrophils of 40 asthmatic and 20 healthy children were successfully obtained, the recovery rate was over 95%, and the cell activity was over 80%. There was higher extracellular free dsDNA in the plasma of symptomatic group (n = 27) than asymptomatic group (n = 22) and healthy group (n = 20; P < .05). Studies on neutrophils from 40 children with asthma found that NO can be produced by adding SNP, PMA, and LPS. SNP could induce NETs with dose‐ and time‐dependent. PMA (160 nM) had the strongest ability to induce NETs, LPS (200 ng/mL) followed, SNP (200 µM) was the weakest (P < .05), and the amount of NETs in the asthma group was significantly higher than that in the healthy group (P < .05). NOS inhibitors had the same blocking capacity for PMA‐ and LPS‐induced NETs (P > .05), while NG‐nitro‐ l‐arginine methyl ester (500 µM) had the strongest inhibitory effect on SNP induction with time‐dependent (P < .05). Inducible NOS was found in the NETs structure.ConclusionChildren with asthma had higher levels of NETs in peripheral blood, especially when they had asthma symptoms. We verified the ability of NO to induce NETs, and found neutrophils from asthmatic children can produce more NETs in vitro. NOS inhibitors blocked this process may provide new therapeutic targets for childhood asthma.

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Fractional Exhaled Nitric Oxide for the Diagnosis of Childhood Asthma: a Systematic Review and Meta-analysis

Tang

Xie

Yuan

et al. 2016

Clinic Rev Allerg Immunol

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The gold standard for diagnosing asthma in children is based on clinical history of respiratory symptoms, physical examination, and respiratory function testing. Recent advances indicate that a non-invasive measure of airway inflammation, fractional exhaled nitric oxide (FeNO), provides objective data for use in asthma diagnosis. However, the diagnostic performance of FeNO in children with asthma has not been clearly defined. This systematic review and meta-analysis aimed to evaluate the diagnostic accuracy of FeNO in the clinical determination of asthma in children. Databases of PubMed, the Cochrane Library, EMBASE, MEDION, and Web of Science were searched for relevant articles through March 31, 2016. A bivariate model was used for pooling estimates of sensitivity, specificity, diagnostic odds ratio (DOR), and area under the summary receiver operating curves (SROC) as the main diagnostic measures. In total, eight studies met the inclusion criteria, which included 2933 subjects. The pooled estimates of sensitivity, specificity, and DOR for the detection of asthma in children were 0.79 [95 % confidence interval (CI), 0.64-0.89], 0.81 (95 % CI, 0.66-0.90), and 16.52 (95 % CI, 7.64-35.71). The SROC was 0.87 (95 % CI, 0.84-0.90). In brief, FeNO achieves a moderate diagnostic performance in the detection of asthma in children.

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An Overview of Fractional Exhaled Nitric Oxide and Children with Asthma

Cited by 27 publications

References 92 publications

Association of polymorphisms of NO synthases and arginase genes, clinical, laboratory and functional indicators with the level of nitrogen oxide in exhaled air in children with bronchial asthma

Association of polymorphisms of NO synthases and arginase genes, clinical, laboratory and functional indicators with the level of nitrogen oxide in exhaled air in children with bronchial asthma

Inhibitors of nitric oxide synthase can reduce extracellular traps from neutrophils in asthmatic children in vitro

Fractional Exhaled Nitric Oxide for the Diagnosis of Childhood Asthma: a Systematic Review and Meta-analysis

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