An overview of emerging hyphenated SEM-EDX and Raman spectroscopy systems: Applications in life, environmental and materials sciences

Cardell, Carolina; Guerra-Tschuschke, Isabel

doi:10.1016/j.trac.2015.12.001

Cited by 66 publications

(42 citation statements)

References 40 publications

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“…The characteristics of biofilm attachment on zeolite surface were observed using Scanning Electron Microscope-Energy Dispersive-X-ray Spectroscopy (SEM-EDX). For SEM the biofilms samples were fixed with 2.5% glutaraldehyde, ethanol (dehydrated) and coated with gold under vacuum in an argon atmosphere [25]. The surface morphology of the gold coated samples was visualized by a SEM (JEOL JSM-T300).…”

Section: Characterization Of Developed Srb Biofilms Populationmentioning

confidence: 99%

Removal of Sulphate and Manganese on Synthetic Wastewater in Sulphate Reducing Bioreactor Using Indonesian Natural Zeolite

Retnaningrum

Wilopo

2017

Indones. J. Chem.

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Section: Characterization Of Developed Srb Biofilms Populationmentioning

confidence: 99%

Removal of Sulphate and Manganese on Synthetic Wastewater in Sulphate Reducing Bioreactor Using Indonesian Natural Zeolite

Retnaningrum

Wilopo

2017

Indones. J. Chem.

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“…Laser Raman spectroscopy plays an increasing important role in nanobiotechnology, [1] nanomaterials, [2] environmental sciences, medicine, and life sciences. [3] Based on vibrational transitions, it has long been regarded as a valuable nondestructive tool for the identification of chemical and biological samples as well as the elucidation of molecular structure, showing many advantages compared to other spectroscopic techniques, such as ultraviolet-visible spectroscopy (UV-Vis) absorption in terms of selectivity and sampling. However, the Raman signals are inherently weak and the detection of the analyte at low concentration range is limited.…”

Section: Introductionmentioning

confidence: 99%

Quantitative surface‐enhanced resonance Raman scattering analysis of methylene blue using silver colloid

Anastasopoulos

Beobide

Manikas

et al. 2017

J Raman Spectroscopy

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Quantitative analysis of methylene blue (MB) has been performed with Surface Enhanced Raman Scattering, SERS, using citrate reduced silver colloid activated with NaCl. In this study, the combination of SERS occurring at the proximity of the plasmon surface and of resonance Raman with excitation wavelength matched to the maximum absorbance of the molecule being analyzed, was successfully applied to maximize the Raman scattering intensity. The surface‐enhanced resonance Raman scattering (SERRS) spectra of aqueous solutions of the dye at various concentrations were collected in right‐angle scattering geometry configuration which results favorable when nanocolloidal dispersions are used. The MB concentration was correlated with the intensity of the peak centered at 1,625 cm−1 in the SERRS spectrum and this correlation resulted in an extremely sensitive quantitative investigation of the MB with limit of detection in pM level. Among others, this method can be easily applied for the examination of both the selectivity of waste water purification membranes for relevant small molecular weight contaminants or/and the solar photocatalytic effectiveness of such membranes for the degradation of pertinent pollutants by their quantitative assessment in the permeate at very low concentration range.

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“…According to literature, there are diverse configurations available to combine SEM and Raman spectroscopy as is shown in Figure 1. [8] In contrast to an "on axis" principle ( Figure 1a), where the Raman optics measures using the position below the pole piece of the SEM, the RISE microscope is based on the "off axis" system, a setup where the axes of the Raman microscopeś objective lens and the SEḾs electron column are parallel but not identical ( Figure 1b). The downside of an "off axis" system is that the sample has to be precisely transferred between the Raman position and the SEM position.…”

Section: Correlative Microscopy à the New System Rise Combined With Edxsmentioning

confidence: 99%

“…a) SEM‐Raman microscopy system based on an “on axis” priciple, the Raman signal is measured directly under the SEM pole piece. b) Correlative SEM and Raman system, where the Raman optics is located outside the SEM electron beam axis; measurement takes place sequentially after a precise displacement of the stage …”

Section: Introductionmentioning

confidence: 99%

The Combination of Electron Microscopy, Raman Microscopy and Energy Dispersive X‐Ray Spectroscopy for the Investigation of Polymeric Materials

Schmidt

Fitzek

Nachtnebel

et al. 2019

Macromolecular Symposia

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Polymers play an important role in materials science, because of their advantages over other types of materials. The huge variety in terms of the types, arrangements and combinations of monomers found in polymers leads to an extremely wide diversity of different polymeric materials. Although there is a wide range of analytical techniques to characterize polymeric materials, here the focus is laid on a novel correlative microscopy method, combining Raman microscopy, scanning electron microscopy (SEM) and energy dispersive X‐ray spectroscopy (EDXS). Therefore, three polymeric specimens were analysed by the new system RISE (Raman Imaging and Scanning Electron microscopy) in combination with EDXS. The aim of this contribution is to demonstrate the potential of the new system to characterize different polymeric layers, as well as fillers and additives in polymeric materials. In addition to the results, a methodical chapter deals with preparation aspects, investigation skills and imaging modes of the electron microscope. Here the so‐called variable pressure mode (VP) is mentioned, where nitrogen is used as imaging gas at pressures between 10 and 133 Pa. Furthermore, the low‐voltage mode in the high vacuum is discussed which gives a better surface resolution due to a smaller interaction volume of the electrons in the material and furthermore causes lower beam damage. Both modes work without a conductive coating on the specimen, which is a requirement for Raman investigations. Further capabilities of the RISE system in combination with the EDXS will be discussed afterwards.

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An overview of emerging hyphenated SEM-EDX and Raman spectroscopy systems: Applications in life, environmental and materials sciences

Cited by 66 publications

References 40 publications

Removal of Sulphate and Manganese on Synthetic Wastewater in Sulphate Reducing Bioreactor Using Indonesian Natural Zeolite

Removal of Sulphate and Manganese on Synthetic Wastewater in Sulphate Reducing Bioreactor Using Indonesian Natural Zeolite

Quantitative surface‐enhanced resonance Raman scattering analysis of methylene blue using silver colloid

The Combination of Electron Microscopy, Raman Microscopy and Energy Dispersive X‐Ray Spectroscopy for the Investigation of Polymeric Materials

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