An Overview of DNA Typing Methods for Human Identification: Past, Present, and Future

Thompson, Robyn; Zoppis, Silvia; McCord, Bruce

doi:10.1007/978-1-61779-461-2_1

Cited by 41 publications

(27 citation statements)

References 39 publications

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“…Although DNA analysis technologies are widely used, CE-based genotyping on all biological evidence present at the scene of a crime remains laborious (Roewer, 2009;Thompson et al, 2012). In many cases, a large number of evidence materials are needed for quick screening.…”

Section: Discussionmentioning

confidence: 99%

“…Over the last decade, automated capillary electrophoresis (CE) systems with fluorescence-labeled primers have been widely used in STR genotyping. Although CE significantly improved the throughput and automation, the procedure is costly and requires laborious post-PCR handling (Ziętkiewicz et al, 2012;Thompson et al, 2012). Additionally, electrophoresis-based methods only differentiate genotypes of length polymorphisms, while nucleotide variations such as single nucleotide polymorphisms (SNPs) in the flanking sequences of a repeat motif cannot be detected.…”

Section: Introductionmentioning

confidence: 99%

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Y-STR genetic screening by high-resolution melting analysis

et al. 2016

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ABSTRACT. Currently, the widely used automated capillary electrophoresis-based short tandem repeat (STR) genotyping method for genetic screening in forensic practice is laborious, time-consuming, expensive, and technically challenging in some cases. Thus, new molecular-based strategies for conclusively identifying forensically relevant biological evidence are required. Here, we used high-resolution melting analysis (HRM) for Y-chromosome STR genotyping for forensic genetic screening. The reproducibility of the melting profile over dilution, sensitivity, discrimination power, and other factors was preliminarily studied in 10 Y-STR loci. The results showed that HRM-based approaches revealed more genotypes (compared to capillary electrophoresis), showed higher uniformity in replicate tests and diluted samples, and enabled successful detection of DNA at concentrations as low as 0.25 ng. For mixed samples, the melting curve profiles discriminated between mixed samples based on reference samples with high efficiency. The triplex Y-chromosome STR HRM assay was performed and provided a foundation for further studies such as a multiplex HRM assay. The HRM approach is a one-step application and the entire procedure can be completed within 2 h at a low cost. In conclusion, our findings demonstrate that the HRM-based Y-STR assay is a useful screening tool that can be used in forensic practice.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Y-STR genetic screening by high-resolution melting analysis

et al. 2016

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“…In order to accurately identify cell lines, analysis of highly polymorphic DNA sequences via DNA fingerprinting is commonly employed (Thompson et al, 2012). The highly polymeric short tandem repeat (STR) loci are amplified using PCR and the products analyzed at high resolution through capillary gel electrophoresis (Butler, 2006 The GCCP task force report introduces laboratory user responsibilities and the appropriate procedures necessary for the purchase, installation, commissioning, correct use, performance monitoring and maintenance (Coecke et al, 2005).…”

Section: Additional Tests A) Cell Line Identitymentioning

confidence: 99%

Good Cell Culture Practice for stem cells and stem-cell-derived models

Pamies

2017

Toxicology Letters

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SummaryThe first guidance on Good Cell Culture Practice (GCCP) dates back to 2005. This document expands this to include aspects of quality assurance for in vitro cell culture focusing on the increasingly diverse cell types and culture formats used in research, product development, testing and manufacture of biotechnology products and cell-based medicines. It provides a set of basic principles of best practice that can be used in training new personnel, reviewing and improving local procedures, and helping to assure standard practices and conditions for the comparison of data between laboratories and experimentation performed at different times. This includes recommendations for the documentation and reporting of culture conditions. It is intended as guidance to facilitate the generation of reliable data from cell culture systems, and is not intended to conflict with local or higher level legislation or regulatory requirements. It may not be possible to meet all recommendations in this guidance for practical, legal or other reasons. However, when it is necessary to divert from the principles of GCCP, the risk of decreasing the quality of work and the safety of laboratory staff should be addressed and any conclusions or alternative approaches justified. This workshop report is considered a first step toward a revised GCCP 2.0.

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“…Sometimes these standard samples are not available for comparison of DNA profiles. In such a scenario, nontraditional samples may be used as reference samples [4]. Ideally, any item which comes into contact with the human body can be used as a potential source of DNA, and that can then be used for genetic profiling.…”

Section: Introductionmentioning

confidence: 99%

The Role of Lipsticks and Blush Sticks in Genetic Profiling for Human Identification

Rasool

Ahmad

2016

AJFSFM

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ware version 1.0. The quantification results showed that the yield of DNA obtained from lipstick samples was greater than that of DNA obtained from blush stick samples. The real-time PCR results showed that only 16% of cosmetic samples had shown inhibition. The DNA profiles obtained from all blush stick samples were of good quality compared to those from lipstick samples. No profile was obtained from one blush stick sample (DNA 0.001 ng/µL) and four lipstick samples (DNA 0.001-0.003 ng/µL) because the amount of DNA in each of these samples was less than the amount required for successful amplification. DNA profiles were successfully generated from most of the samples of various available brands of lipsticks and blush sticks. This is the first study proving that DNA profiles can be generated from various lip and face cosmetics. AbstractThe core objectives of the current study are to generate human DNA profiles from used lipsticks and blush sticks of various brands available in Pakistan. A total of 12 international and local brands of lipsticks and blush sticks were selected. The lipsticks and blush sticks were applied by twenty different healthy female volunteers of 21-30 years of age. The heads of used lipsticks and blush sticks were swabbed with dry sterile cotton swabs. The qualitative and quantitative analysis was done by real time polymerase chain reaction (PCR), using a Quantifiler® Duo DNA Quantification Kit on Real Time PCR ABI™ 7500. Samples were amplified for 16 STR loci using an AmpFlSTR® Identifiler® PCR amplification kit on Thermocycler ABI 9700. The amplified product was run on Applied Biosystems 3130™ Genetic Analyzer. The genetic profiles were analyzed on GeneMapper® ID-X soft-

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An Overview of DNA Typing Methods for Human Identification: Past, Present, and Future

Cited by 41 publications

References 39 publications

Y-STR genetic screening by high-resolution melting analysis

Y-STR genetic screening by high-resolution melting analysis

Good Cell Culture Practice for stem cells and stem-cell-derived models

The Role of Lipsticks and Blush Sticks in Genetic Profiling for Human Identification

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