An Overview of Diverse Strategies To Inactivate
            <i>Enterobacteriaceae</i>
            -Targeting Bacteriophages

Raza, Sada; Wdowiak, Mateusz; Paczesny, Jan

doi:10.1128/ecosalplus.esp-0019-2022

Cited by 3 publications

(6 citation statements)

References 284 publications

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“…T4 is a good model for studying antiphagents (i.e., antibacteriophage agents), which is crucial from the biotechnology point of view, where active compounds are produced in bacteria. 36 The phages were exposed to the pieces of titanium foil coated with CuL, CuL-PVP, and CuL-D(+)G. The pieces were of identical size. As controls, we analyzed phage suspension and phage suspension exposed to the substrate (titanium foil).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…It comprises a capsid (protein shell with DNA inside), a tail, and fibers. T4 is a good model for studying antiphagents (i.e., antibacteriophage agents), which is crucial from the biotechnology point of view, where active compounds are produced in bacteria …”

Section: Results and Discussionmentioning

confidence: 99%

“…and its morphology is much simpler. MS2 is considered a good surrogate for studies of viruses infecting eukaryotic cells . It was used as a model to study COVID-19, , human norovirus, and other enteric viruses .…”

Section: Results and Discussionmentioning

confidence: 99%

“…MS2 is considered a good surrogate for studies of viruses infecting eukaryotic cells. 36 It was used as a model to study COVID-19, 37 , 38 human norovirus, 39 and other enteric viruses. 40 It is also a challenging target, as it can withstand the action of agents deactivating other phages.…”

Section: Results and Discussionmentioning

confidence: 99%

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Copper Oxide Electrochemical Deposition to Create Antiviral and Antibacterial Nanocoatings

Kusior,

Mazurkow,

Jelen

et al. 2024

Langmuir

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The impact of the reaction environment on the formation of the polycrystalline layer and its biomedical (antimicrobial) applications were analyzed in detail. Copper oxide layers were synthesized using an electrodeposition technique, with varying additives influencing the morphology, thickness, and chemical composition. Scanning electron microscopy (SEM) images confirmed the successful formation of polyhedral structures. Unmodified samples (CuL) crystallized as a mixture of copper oxide (I) and (II), with a thickness of approximately 1.74 μm. The inclusion of the nonconductive polymer polyvinylpyrrolidone (PVP) during synthesis led to a regular and compact CuO-rich structure (CuL-PVP). Conversely, adding glucose resulted in forming a Cu 2 O-rich nanostructured layer (CuL-D(+)G). Both additives significantly reduced the sample thickness to 617 nm for CuL-PVP and 560 nm for CuL-D(+)G. The effectiveness of the synthesized copper oxide layers was demonstrated in their ability to significantly reduce the T4 phage titer by approximately 2.5−3 log. Notably, CuL-PVP and CuL-D(+)G showed a more substantial reduction in the MS2 phage titer, achieving about a 5-log decrease. In terms of antibacterial activity, CuL and CuL-PVP exhibited moderate efficacy against Escherichia coli, whereas CuL-D(+)G reduced the E. coli titer to undetectable levels. All samples induced similar reductions in Staphylococcus aureus titer. The study revealed differential susceptibilities, with Gram-negative bacteria being more vulnerable to CuL-D(+)G due to its unique composition and morphology. The antimicrobial properties were attributed to the redox cycling of Cu ions, which generate ROS, and the mechanical damage caused by nanostructured surfaces. A crucial finding was the impact of surface composition rather than surface morphology on antimicrobial efficacy. Samples with a dominant Cu 2 O composition exhibited potent antibacterial and antiviral properties, whereas CuO-rich materials showed predominantly enhanced antiviral activity. This research highlights the significance of phase composition in determining the antimicrobial properties of copper oxide layers synthesized through electrodeposition.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Copper Oxide Electrochemical Deposition to Create Antiviral and Antibacterial Nanocoatings

Kusior,

Mazurkow,

Jelen

et al. 2024

Langmuir

Self Cite

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“…Developing new methods for protection against phages will be crucial in the following years. The most commonly used physical and chemical methods are listed and described in a review by Raza et al [ 112 ].…”

Section: Future Outlook: New Antiphagentsmentioning

confidence: 99%

Bacteriophage Challenges in Industrial Processes: A Historical Unveiling and Future Outlook

Kamiński,

Paczesny

2024

Pathogens

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Humans have used fermentation processes since the Neolithic period, mainly to produce beverages. The turning point occurred in the 1850s, when Louis Pasteur discovered that fermentation resulted from the metabolism of living microorganisms. This discovery led to the fast development of fermented food production. The importance of industrial processes based on fermentation significantly increased. Many branches of industry rely on the metabolisms of bacteria, for example, the dairy industry (cheese, milk, yogurts), pharmaceutical processes (insulin, vaccines, antibiotics), or the production of chemicals (acetone, butanol, acetic acid). These are the mass production processes involving a large financial outlay. That is why it is essential to minimize threats to production. One major threat affecting bacteria-based processes is bacteriophage infections, causing substantial economic losses. The first reported phage infections appeared in the 1930s, and companies still struggle to fight against phages. This review shows the cases of phage infections in industry and the most common methods used to prevent phage infections.

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The effect of thyme essential oil and endothelial progenitor stem cells on lipopolysaccharide‐induced sepsis in C57BL/6 mice

Soltan Dallal,

Siavashi,

Karimaei

et al. 2024

Biotech and App Biochem

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Sepsis is a potentially fatal syndrome related to severe systemic inflammation developed by infection. Despite different antimicrobial therapies, morbidity and mortality rates remain high. Herbs along with cell therapy have been introduced as a promising option to improve the symptoms of sepsis. The present study aimed to evaluate the therapeutic effect of simultaneous administration of thyme essential oil (TEO) and endothelial progenitor stem cells (EPCs) on lipopolysaccharide (LPS)‐induced sepsis in C57BL/6 mice. Sepsis was induced in C57Bl/6J mice by intraperitoneal injection of LPS, followed 2 h later by an intravenous injection of EPCs or oral administration of TEO or simultaneous administration of TEO and EPCs. After 10 days, the complete blood cell, renal and liver factors, serum levels of inflammatory cytokines, and angiogenic factors were measured. Simultaneous treatment with EPCs and TEO significantly increased the survival of mice with sepsis and modulated the inflammatory response by reducing the serum levels of pro‐inflammatory cytokines. Moreover, this treatment significantly reduced the level of white blood cells and neutrophils and increased the number of red blood cells, the percentage of hematocrit, and hemoglobin. The combination of TEO with EPCs decreased organ injuries and was assessed by lower levels of the liver enzymes alanine aminotransferase and aspartate aminotransferase compared to the sepsis group. Administration of EPCs and TEO also significantly improved angiogenic factors, lung function, and toll‐like receptor 4 expression. EPCs in combination with TEO increase survival in the LPS‐induced sepsis mice model by acting on several targets. Thus, the combination of TEO with EPCs can be a feasible approach for the future clinical treatment and control of sepsis.

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An Overview of Diverse Strategies To Inactivate Enterobacteriaceae -Targeting Bacteriophages

Abstract: Bacteriophages are viruses that infect bacteria and thus threaten industrial processes relying on the production executed by bacterial cells. Industries bear huge economic losses due to such recurring and resilient infections.

Cited by 3 publications

References 284 publications

Copper Oxide Electrochemical Deposition to Create Antiviral and Antibacterial Nanocoatings

Copper Oxide Electrochemical Deposition to Create Antiviral and Antibacterial Nanocoatings

Bacteriophage Challenges in Industrial Processes: A Historical Unveiling and Future Outlook

The effect of thyme essential oil and endothelial progenitor stem cells on lipopolysaccharide‐induced sepsis in C57BL/6 mice

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