An Overview of Commercial and Technological Limitations to Marketing of Micropropagated Plants

Ilan, A.; Khayat, E.

doi:10.17660/actahortic.1997.447.127

Cited by 9 publications

(10 citation statements)

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“…Most of the nearly one billion commercially micropropagated plants are grown in gel-filled boxes, transferred by hand, and set into humidity tents in a greenhouse to prepare them for the market (Ilan and Khayat, 1997). The in vitro environment provides plant cultures with low light intensity, high relative humidity, relatively constant temperatures, and large fluctuations in CO 2 (Kozai, 1991).…”

Section: Introductionmentioning

confidence: 99%

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One‐step acclimatization of plantlets using a mist reactor

Correll

Weathers²

2001

Biotech & Bioengineering

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A mist reactor was used to grow and acclimatize carnation plants in vitro without using ex vitro acclimatization techniques. The acclimatization protocol in the reactor consisted of altering the mist-on period during the course of the culture period and a stepwise reduction in the relative humidity surrounding the plants from 98% to 70% relative humidity (RH) during the final week of in vitro growth. After transfer and further growth in a greenhouse for 5 weeks, survival was 91% for plants grown in reactors, 81% from vented boxes, and 50% from unvented boxes. Ex vitro survival directly correlated with increased in vitro rooting and decreased hyperhydration. In vitro rooting also correlated with high-quality plants, but did not significantly correlate with low hyperhydration, as normal plants often lacked roots. After 5 weeks in the greenhouse, the quantity of mid- and high-quality plants obtained from reactors and ventilated boxes was similar. Conditions in the mist reactor can be manipulated to produce plants that are readily acclimatized and are equal or better in quality and yield than plants produced using conventional methods.

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Section: Introductionmentioning

confidence: 99%

“…Once removed from culture, plants must gradually adapt to low humidity, high light intensities, and large temperature fluctuations through ex vitro acclimatization techniques (Preece and Sutter, 1991). These techniques, however, are expensive, time-consuming, and laborintensive (Ilan and Khayat, 1997;Fila et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

One‐step acclimatization of plantlets using a mist reactor

Correll

Weathers²

2001

Biotech & Bioengineering

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“…Worldwide, an estimated one billion plants per year are produced by micropropagation [14]. In the micropropagation scheme (Figure 4), [15,16], stage 0 is the selection of the donor plant, and may involve genetic testing and disease indexing.…”

Section: Mist Reactors For Micropropagationmentioning

confidence: 99%

“…Taken together, these studies show that hyperhydration can be reduced or eliminated using a mist reactor where gas content is regulated. Acclimatization accounts for approximately 30% of the total production cost of micropropagation [14]. Correll and Weathers [11] used a mist reactor to grow and acclimatize carnation plants in vitro without using ex vitro acclimatization techniques, which are expensive, time-consuming, and labour-intensive [14,31].…”

Section: Mist Reactors For Micropropagationmentioning

confidence: 99%

“…Acclimatization accounts for approximately 30% of the total production cost of micropropagation [14]. Correll and Weathers [11] used a mist reactor to grow and acclimatize carnation plants in vitro without using ex vitro acclimatization techniques, which are expensive, time-consuming, and labour-intensive [14,31]. Ex vitro plant survival rates were higher for plants grown in the mist reactor (91% survival) using the acclimatization protocol described in Correll and Weathers [11] versus a conventional propagation system (GA-7 culture boxes) that only had a 50% survival rate.…”

Section: Mist Reactors For Micropropagationmentioning

confidence: 99%

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