An Overview of Cell Membrane Perforation and Resealing Mechanisms for Localized Drug Delivery

He, Stephanie; Singh, Davindra; Helfield, Brandon

doi:10.3390/pharmaceutics14040886

Cited by 14 publications

(14 citation statements)

References 162 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We believe that the large time difference (up to couple of hundreds seconds) compared to our findings may result from the difference of the mechanisms of membrane perforation, pore size, cell type, and environmental factors 31 . Also, according to He et al., it is reasonable that the viable cell membrane resealing occurring within seconds to minutes 26 …”

Section: Discussioncontrasting

confidence: 43%

“…To achieve rapid repair and control perforation dynamics of porated cells, the mechanism of cell membrane self-repair, such as how to modulate the membrane resealing dynamics to enhance therapeutic efficiency, has been studied for many years 26 in which real-time monitoring of the perforated process on mammalian cells has played a very important role. For example, Schneckenburger et al 6 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Monitoring optoporated process on mammalian cells by real-time measurement of membrane resealing time

Wang

et al. 2023

J. Biomed. Opt.

View full text Add to dashboard Cite

. Significance Resealing time based loading efficiency of optoporation is the key parameter for drug or gene delivery. This work describes a comparatively simple optical approach to directly measure the cell membrane resealing time of the gold nanoparticle mediated photoporation. Aim To establish a membrane potential detection optical system, which can provide a direct measurement of resealing time of the optoporated cells. Approach Voltage sensitive dye has been used to label the gold nanoparticle covered cell before laser activation and the resealing time was estimated from the voltage change due to the fluorescence light intensity change before and after laser activation. The approach has been validated by the simulated data based on diffusion model and Monte Carlo simulation and the experimental data obtained from a flow cytometry analysis. Results The measured resealing time after perforation varied from 28.6 to 163.8 s on Hela cells when the irradiation fluence was increased, with a correlation coefficient ( ) of 0.9938. This result is in agreement with the resealing time (1-2 min) of photothermal porated Hela cells measured by electrical impedance method. The intracellular delivery efficiency of extracellular macromolecular under the same irradiation fluence depends mainly on diffusion velocity rather than pore size. Conclusion The method described here can be used to directly measure resealing time of optoporated cells for accurately estimating the loading efficiency on discovering the mechanism of optoporation.

show abstract

Section: Discussioncontrasting

confidence: 43%

Section: Introductionmentioning

confidence: 99%

Monitoring optoporated process on mammalian cells by real-time measurement of membrane resealing time

Wang

et al. 2023

J. Biomed. Opt.

View full text Add to dashboard Cite

show abstract

“…The study of cell wound repair has considerable clinical relevance. Pharmaceutical delivery methods and technologies are a popular and growing field of research that relies heavily on an understanding of the fundamental mechanisms of local and reversible plasma membrane disruptions to design effective methods of drug delivery [ 137 , 138 , 139 ]. Understanding the full spectrum of molecular mechanisms underpinning cell wound repair will provide important new insights into the many critical cell behaviors and fundamental biological regulations that take place during cellular events in daily life, and in pathological states from infections to diseases/cancers.…”

Section: Discussionmentioning

confidence: 99%

Wrangling Actin Assemblies: Actin Ring Dynamics during Cell Wound Repair

Hui

Stjepić

Nakamura

et al. 2022

Cells

View full text Add to dashboard Cite

To cope with continuous physiological and environmental stresses, cells of all sizes require an effective wound repair process to seal breaches to their cortex. Once a wound is recognized, the cell must rapidly plug the injury site, reorganize the cytoskeleton and the membrane to pull the wound closed, and finally remodel the cortex to return to homeostasis. Complementary studies using various model organisms have demonstrated the importance and complexity behind the formation and translocation of an actin ring at the wound periphery during the repair process. Proteins such as actin nucleators, actin bundling factors, actin-plasma membrane anchors, and disassembly factors are needed to regulate actin ring dynamics spatially and temporally. Notably, Rho family GTPases have been implicated throughout the repair process, whereas other proteins are required during specific phases. Interestingly, although different models share a similar set of recruited proteins, the way in which they use them to pull the wound closed can differ. Here, we describe what is currently known about the formation, translocation, and remodeling of the actin ring during the cell wound repair process in model organisms, as well as the overall impact of cell wound repair on daily events and its importance to our understanding of certain diseases and the development of therapeutic delivery modalities.

show abstract

“…Flores-Romero et al (21) reported that pyroptosis and necroptosis may cause an increase in the number and size of cell pores. There are studies suggesting that increased cell membrane permeabilization may be an effective adjuvant in new cancer treatments (22,23). Bleb formation on the cell membrane usually indicates apoptotic cell death (24).…”

Section: Discussionmentioning

confidence: 99%

Hiperbarik oksijen tedavisinin MCF-7 meme kanseri hücrelerinin morfolojik özellikleri ve sağkalımı üzerindeki etkileri

et al. 2023

View full text Add to dashboard Cite

Aim: This study aims to determine the effects of hyperbaric oxygen therapy at different pressure values on cell morphology and cell survival in the MCF-7 breast cancer cell line. Materials and Methods: The experimental groups were formed by applying 100% oxygen to MCF-7 breast cancer cells at 1.5, 2, and 2.5 atmospheres for 2 hours. The control group did not receive treatment. At the end of the experiment, cell survival was investigated by CCK-8 analysis, cell shapes were determined by cresyl violet staining, and cell surface morphologies were determined by scanning electron microscope. Results: Cell viability was significantly reduced at atmospheric pressure of 1.5, 2, and 2.5 compared to the control group (p < 0.005). As pressure increased, the surface area of the cell decreased, nuclear condensation increased, and the cell borders became irregular. Cell membrane bleb and cell membrane porosity increased at 2 and 2.5 atmospheres. Conclusion: Hyperbaric oxygen therapy severely reduces the viability of MCF-7 breast cancer cells under increased pressure. It can induce apoptosis and change the shape and surface morphology of MCF-7 breast cancer cells. Although further studies are needed, our study supports the potential use of hyperbaric oxygen therapy in the treatment of breast cancer.

show abstract

An Overview of Cell Membrane Perforation and Resealing Mechanisms for Localized Drug Delivery

Cited by 14 publications

References 162 publications

Monitoring optoporated process on mammalian cells by real-time measurement of membrane resealing time

Monitoring optoporated process on mammalian cells by real-time measurement of membrane resealing time

Wrangling Actin Assemblies: Actin Ring Dynamics during Cell Wound Repair

Hiperbarik oksijen tedavisinin MCF-7 meme kanseri hücrelerinin morfolojik özellikleri ve sağkalımı üzerindeki etkileri

Contact Info

Product

Resources

About