An Overview of Advanced <i>In Vitro</i> Corneal Models: Implications for Pharmacological Testing

Meenen, Joris Van; Dhubhghaill, Sorcha Ní; Bogerd, Bert Van den; Koppen, Carina

doi:10.1089/ten.teb.2021.0031

Cited by 5 publications

(8 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spherical cellular aggregates, such as spheroids, derived from primary tissue and composed of a single cell type, improve 2D cultures by creating a 3D environment with greater cell–cell interactions. Consequently, cell behavior in response to therapeutic treatments resembles that of native tissue more closely. − It can take up to a month for mature corneal spheres to form, with differentiation times varying greatly between studies and being related to the type of cell used for spheroid development . Spheroids, notable larger spheres composed of various stages of cells, are commonly used in oncology because they represent small solid tumors. , These large spheres are the result of limited nutrition transport, hypoxia, and waste accumulation in the interior regions of large spheroids, which may influence cell behavior .…”

Section: In Vitro Reconstructed Corneal Tissue Models To Study Drug P...mentioning

confidence: 99%

“…Consequently, cell behavior in response to therapeutic treatments resembles that of native tissue more closely. − It can take up to a month for mature corneal spheres to form, with differentiation times varying greatly between studies and being related to the type of cell used for spheroid development . Spheroids, notable larger spheres composed of various stages of cells, are commonly used in oncology because they represent small solid tumors. , These large spheres are the result of limited nutrition transport, hypoxia, and waste accumulation in the interior regions of large spheroids, which may influence cell behavior . In addition, they can be used to investigate drug absorption, drug response, and cell–cell interactions, amongst others.…”

Section: In Vitro Reconstructed Corneal Tissue Models To Study Drug P...mentioning

confidence: 99%

“…In ophthalmology, corneal spheroids were primarily used for extracting highly proliferative cells through sphere formation experiments and have only recently been applied to permeability and cellular uptake studies. The focus of these studies was on the potential of mucoadhesive voriconazole-loaded nanomicelles for the treatment of fungal keratitis and the time-dependent penetration efficiency of moxifloxacin-loaded polymeric nanoparticles for the treatment of bacterial keratitis. , Weng et al evaluated the efficacy of cRGD-functionalized nanomicelles encapsulating flurbiprofen for the treatment of ocular inflammation.…”

Section: In Vitro Reconstructed Corneal Tissue Models To Study Drug P...mentioning

confidence: 99%

“…In addition, the nanoformulation significantly enhanced anti-inflammatory efficacy in the eye . However, spheroids are less ideal for mimicking the ordered structure of the cornea due to their cancer-like features, which is essential for proper pharmacological testing …”

Section: In Vitro Reconstructed Corneal Tissue Models To Study Drug P...mentioning

confidence: 99%

See 3 more Smart Citations

In Vitro and Ex Vivo Models for Assessing Drug Permeation across the Cornea

et al. 2023

View full text Add to dashboard Cite

Drug permeation across the cornea remains a major challenge due to its unique and complex anatomy and physiology. Static barriers such as the different layers of the cornea, as well as dynamic aspects such as the constant renewal of the tear film and the presence of the mucin layer together with efflux pumps, all present unique challenges for effective ophthalmic drug delivery. To overcome some of the current ophthalmic drug limitations, the identification and testing of novel drug formulations such as liposomes, nanoemulsions, and nanoparticles began to be considered and widely explored. In the early stages of corneal drug development reliable in vitro and ex vivo alternatives, are required, to be in line with the principles of the 3Rs (Replacement, Reduction, and Refinement), with such methods being in addition faster and more ethical alternatives to in vivo studies. The ocular field remains limited to a handful of predictive models for ophthalmic drug permeation. In vitro cell culture models are increasingly used when it comes to transcorneal permeation studies. Ex vivo models using excised animal tissue such as porcine eyes are the model of choice to study corneal permeation and promising advancements have been reported over the years. Interspecies characteristics must be considered in detail when using such models. This review updates the current knowledge about in vitro and ex vivo corneal permeability models and evaluates their advantages and limitations.

show abstract

Section: In Vitro Reconstructed Corneal Tissue Models To Study Drug P...mentioning

confidence: 99%

Section: In Vitro Reconstructed Corneal Tissue Models To Study Drug P...mentioning

confidence: 99%

Section: In Vitro Reconstructed Corneal Tissue Models To Study Drug P...mentioning

confidence: 99%

Section: In Vitro Reconstructed Corneal Tissue Models To Study Drug P...mentioning

confidence: 99%

See 2 more Smart Citations

In Vitro and Ex Vivo Models for Assessing Drug Permeation across the Cornea

et al. 2023

View full text Add to dashboard Cite

show abstract

“…This was followed by corneal selection at day 30 and eventual maturation at >120 days [ 79 ]. In addition, so-called organ chips may also represent an interesting model, which can be alternatively used in the laboratory to study eye disorders in vitro [ 52 , 80 ]. Rötzer et al (2019) recently presented an interesting ex vivo slice culture model using ‘thicker’ (300 µm) tissue sections of donor eyelids in culture.…”

Section: Development Of 3d Cell Culture Modelsmentioning

confidence: 99%

The Communication between Ocular Surface and Nasal Epithelia in 3D Cell Culture Technology for Translational Research: A Narrative Review

Aydin

Dietrich

Witt

et al. 2021

IJMS

View full text Add to dashboard Cite

There is a lack of knowledge regarding the connection between the ocular and nasal epithelia. This narrative review focuses on conjunctival, corneal, ultrastructural corneal stroma, and nasal epithelia as well as an introduction into their interconnections. We describe in detail the morphology and physiology of the ocular surface, the nasolacrimal ducts, and the nasal cavity. This knowledge provides a basis for functional studies and the development of relevant cell culture models that can be used to investigate the pathogenesis of diseases related to these complex structures. Moreover, we also provide a state-of-the-art overview regarding the development of 3D culture models, which allow for addressing research questions in models resembling the in vivo situation. In particular, we give an overview of the current developments of corneal 3D and organoid models, as well as 3D cell culture models of epithelia with goblet cells (conjunctiva and nasal cavity). The benefits and shortcomings of these cell culture models are discussed. As examples for pathogens related to ocular and nasal epithelia, we discuss infections caused by adenovirus and measles virus. In addition to pathogens, also external triggers such as allergens can cause rhinoconjunctivitis. These diseases exemplify the interconnections between the ocular surface and nasal epithelia in a molecular and clinical context. With a final translational section on optical coherence tomography (OCT), we provide an overview about the applicability of this technique in basic research and clinical ophthalmology. The techniques presented herein will be instrumental in further elucidating the functional interrelations and crosstalk between ocular and nasal epithelia.

show abstract

Advances in Organoid Technology: A Focus on Corneal Limbal Organoids

Lu,

2024

Stem Cell Rev and Rep

View full text Add to dashboard Cite

An Overview of Advanced In Vitro Corneal Models: Implications for Pharmacological Testing

Cited by 5 publications

References 65 publications

In Vitro and Ex Vivo Models for Assessing Drug Permeation across the Cornea

In Vitro and Ex Vivo Models for Assessing Drug Permeation across the Cornea

The Communication between Ocular Surface and Nasal Epithelia in 3D Cell Culture Technology for Translational Research: A Narrative Review

Advances in Organoid Technology: A Focus on Corneal Limbal Organoids

Contact Info

Product

Resources

About