An overview and comparison of a recombinant antigen-binding fragment and an antigen-binding fragment from a monoclonal antibody against wogonin glucuronide

Abstract

Wogonin glucuronide (wogonin 7-O-β-D-glucuronide, Wgn) is widely recognized as a constituent of Scutellariae radix, which is used in Kampo medicines. Wgn has been used for both pharmacological (antifebrile uses and in detoxification) and research purposes. A recombinant antigen-binding fragment (rFab) and an antigen-binding fragment from a monoclonal antibody (mFab) against Wgn were constructed and used in an indirect competitive enzyme-linked immunosorbent assay (icELISA) in this study. The rFab and mFab against Wgn showed both activity and recognition against Wgn. The developed icELISA was validated as a quantitative analytical method to detect Wgn by testing both its utility and its reliability using multiple concentrations of Wgn from S. radix. This approach provides a more economic method to analyze and purify Kampo medicines.

Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites

“…Thus far, various scFvs against plant secondary metabolites have been constructed and expressed in E. coli to develop icELISA, including plumbagin [ 61 ], G-Re [ 62 ], DZ [ 63 ], wogonin glucuronide [ 64 ], and paclitaxel [ 65 ]. Similarly, Fab-based icELISA has been reported for artemisinin, which is produced from traditional Chinese herbal medicines, e.g., Artemisia annua L. and wogonin glucuronide, for their determination [ 66 , 67 ]. They can be genetically engineered; therefore, fluorescent single-domain antibodies (fluobodies), chimera proteins of a green fluorescent protein (GFP), and an scFv also have been utilized in immunoassays.…”