River Neretva Estuary, located in the middle SouthEastern Adriatic coast, is a very important traditional fi shery ground and biodiversity-rich ichthyologic area. Historically, the estuary situated in Croatia and Bosnia and Herzegovina was composed of wetlands, lakes, and lagoons, but in the 20th century, it was changed into a large agriculture area, settlements and port facilities. The major fl ow was channelled with large banks to prevent fl oods. All these activities enabled signifi cant intrusion of seawater 20 km into the inland area. This led to the marinization of major fl ow freshwater ecosystems. This article presents data on summer fi sh migrations along with the major fl ow of River Neretva, during the May-September period. The temperature and salinity showed that seawater started to enter inland major fl ow from middle May up to the end of September. In total, the 1,429 fi sh individuals were caught. The numerically dominant species were Atherina boyeri (23.58%), Sardina pilchardus (21.62%), Sparus aurata (11.20%), Chelon ramada (8.19%) and Solea solea (7.63%). Dominant species in the total mass were: Chelon ramada (43.45%), Solea solea (14.27%), Sparus aurata (10.14%), Chelon aurata (7.66%) and Anguilla anguilla (3.75%). The major result of this study points to the fact that major Neretva fl ow of 20 km in length from the river mouth is under seasonal summer impact of seawater, which leads to salinization and complete marinization of this ecosystem during the warm period of the year. The marinization of the large Neretva fl ow area creates new nursery and feeding grounds for marine estuarine opportunist fi sh species, such as small pelagics European pilchard and anchovy, and also enlarge these grounds for the marine estuarine dependent species, such as grey mullets, sea breams, and fl atfi shes. Sažetak Ušće rijeke Neretve, smješteno usred jugoistočne obale Jadranskog mora, vrlo je važno tradicionalno ribolovno tlo i ihtiološko područje bogato biološkom raznolikošću. Povijesno, ušće smješteno u Hrvatskoj i Bosni i Hercegovini blio je sastavljeno od močvarnih područja, jezera i laguna, ali u 20. stoljeću pretvoreno je u veliko poljoprivredno područje, naselja i lučke objekte. Glavni tok bio je usmjeren uz pomoć velikih nasipa kako bi se spriječile poplave. Sve ove aktivnosti omogućile su znatan prodor morske vode 20 km u unutrašnjost. To je dovelo do marinizacije slatkovodnih ekosustava glavnih tokova. Ovaj članak daje podatke o ljetnim migracijama ribe u glavnom toku rijeke Neretve, u razdoblju od svibnja do rujna. Temperatura i salinitet pokazali su da je morska voda počela ulaziti u glavni tok u unutrašnjosti od sredine svibnja do kraja rujna. Ukupno je ulovljeno 1429 jedinki ribe. Brojčano dominantne vrste bile su Atherina boyeri (23.58 %), Sardina pilchardus (21.62 %), Sparus aurata (11.20 %), Chelon ramada (8.19 %) i Solea solea (7.63 %). Dominantne vrste u ukupnoj masi bile su: Chelon ramada (43.45 %), Solea solea (14.27 %), Sparus aurata (10.14 %), Chelon aurata (7.66 %) i Anguilla anguilla (3...