An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan.

Takahashi, Kiwao; Okada, Kōsuke; Ohshima, Kan-ichi

doi:10.1292/jvms1939.45.829

Cited by 150 publications

(122 citation statements)

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“…The main clinical sign of PEDV is watery diarrhea with 100% morbidity and 50-100% mortality in piglets up to 1 week of age, and less severe disease in older pigs including sows. Epidemic outbreaks of PEDV have been reported from different countries in Europe as well as Asia, including Japan, China, South Korea and Thailand [[4]–[7]]. Since late 2010, a remarkable increase in PEDV outbreaks has been reported in pig-producing provinces in China resulting in tremendous economic losses attributed to the emergence of new strains [[8]].…”

Section: Introduction Methods and Resultsmentioning

confidence: 99%

Evidence of infectivity of airborne porcine epidemic diarrhea virus and detection of airborne viral RNA at long distances from infected herds

Alonso

Goede

Morrison

et al. 2014

Vet Res

150

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Porcine epidemic diarrhea virus (PEDV) spread rapidly after being diagnosed in the USA in April 2013. In this study we assessed whether PEDV could become airborne and if so, whether the virus was infectious. Air samples were collected both from a room containing experimentally infected pigs and at various distances from the outside of swine farms experiencing acute PEDV outbreaks. Results indicated presence of infectious PEDV in the air from experimentally infected pigs and genetic material of PEDV was detected up to 10 miles downwind from naturally infected farms. Airborne transmission should be considered as a potential route for PEDV dissemination.

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Section: Introduction Methods and Resultsmentioning

confidence: 99%

Evidence of infectivity of airborne porcine epidemic diarrhea virus and detection of airborne viral RNA at long distances from infected herds

Alonso

Goede

Morrison

et al. 2014

Vet Res

150

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“…PEDV has been classified as a member of the Coronaviridae family [1,2]. PED was first detected in Belgium [3] and the UK [4] in 1978, and outbreaks of this disease have been reported in many pig farming countries, leading to severe economic losses in Canada [5], Europe [6,7], and Asia, including Japan [8], China [9], Korea [10], and more recently, Thailand [11]. The complete sequence of the entire genome of the strain CV777 was found to be 28,033 nucleotides in length, after excluding the poly(A) tail [12,13].…”

Section: Introductionmentioning

confidence: 99%

Expression of a Cholera Toxin B Subunit-Neutralizing Epitope of the Porcine Epidemic Diarrhea Virus Fusion Gene in Transgenic Lettuce (Lactuca sativa L.)

2010

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Transgenic plants have been used as a safe and economic expression system for the production of edible vaccines. A synthetic cholera toxin B subunit gene (CTB) was fused with a synthetic neutralizing epitope gene of the porcine epidemic diarrhea virus (sCTB-sCOE), and the sCTB-sCOE fusion gene was introduced into a plant expression vector under the control of the ubiquitin promoter. This plant expression vector was transformed into lettuce (Lactuca sativa L.) using the Agrobacterium-mediated transformation method. Stable integration and transcriptional expression of the sCTB-sCOE fusion gene was confirmed using genomic DNA PCR analysis and northern blot analysis, respectively. The results of western blot analysis with anti-cholera toxin and anti-COE antibody showed the synthesis and assembly of CTB-COE fusion protein into oligomeric structures with pentameric sizing. The biological activity of CTB-COE fusion protein to its receptor, G(M1)-ganglioside, in transgenic plants was confirmed via G(M1)-ELISA with anti-cholera toxin and anti-COE antibody. Based on G(M1)-ELISA, the expression level of CTB-COE fusion proteins reached 0.0065% of the total soluble protein in transgenic lettuce leaf tissues. Transgenic lettuce successfully expressing CTB-COE fusion protein will be tested to induce efficient immune responses against porcine epidemic diarrhea virus infection by administration with raw material.

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“…Since the emergence of PED, disease outbreaks have been reported in several European countries, including Switzerland, Germany, France, the Netherlands, and Bulgaria [2,4]. In Asia, PED was first identified in Japan in 1982 [25], China in 1986 [26], India in 2003, andThailand in 2007 [27]. Unlike in Europe, PED is considered an endemic pathogen in Southeast Asia and has continued to cause a devastating enteric disease and severe economic losses since its first emergence.…”

Section: Introductionmentioning

confidence: 99%

Complete genome characterization of porcine epidemic diarrhea virus in Vietnam

et al. 2015

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Porcine epidemic diarrhea virus (PEDV) first emerged in Vietnam in 2009. In this study, the complete genomes of three Vietnamese PEDV isolates were characterized. These three isolates were isolated from 3-day-old pigs experiencing diarrhea. Two isolates were from swine farms in the south, and the other was from northern Vietnam. The whole genome sequences of these isolates are 28,035 nucleotides in length and have characteristics similar to those of other PEDV isolates. All three Vietnamese PEDV isolates share 99.8 % and 99.6 % sequence identity at the nucleotide and amino acid level, respectively, and have insertions of four amino acids (GENQ) and one amino acid (N) at positions 56-59 and 140, respectively, and one deletion of two amino acids (DG) at positions 160-161. Phylogenetic analysis based on the whole genome revealed that the three Vietnamese PEDV isolates are grouped together with new variants from China from 2011 to 2012 and are genetically distinct from US isolates and the classical PEDV variant. The results suggest that Vietnamese PEDV isolates are new variants, as evidenced by their genetic composition of insertions and a deletion in the spike gene, and they might have originated from the same ancestor as the Chinese PEDV strain. This study provides a better understanding of the molecular characteristics of PEDV in Vietnam.

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An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan.

Cited by 150 publications

References 0 publications

Evidence of infectivity of airborne porcine epidemic diarrhea virus and detection of airborne viral RNA at long distances from infected herds

Evidence of infectivity of airborne porcine epidemic diarrhea virus and detection of airborne viral RNA at long distances from infected herds

Expression of a Cholera Toxin B Subunit-Neutralizing Epitope of the Porcine Epidemic Diarrhea Virus Fusion Gene in Transgenic Lettuce (Lactuca sativa L.)

Complete genome characterization of porcine epidemic diarrhea virus in Vietnam

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