An Outbreak of Suspected Food Poisoning Related to Consumption of Undercooked Deer Meat in Shiga Prefecture

Aoki, Kayo; Hayashi, Masamune; Kouno, Tomomi; Umehara, Seiko; Sakaguchi, Hatsumi; Washida, Kiyoshi; Ishikawa, Kazuhiko

doi:10.5803/jsfm.34.166

Nihon Shokuhin Biseibutsu Gakkai zasshi

2017

DOI: 10.5803/jsfm.34.166

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An Outbreak of Suspected Food Poisoning Related to Consumption of Undercooked Deer Meat in Shiga Prefecture

Kayo Aoki¹,

Masamune Hayashi²,

Tomomi Kouno³

et al.

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Cited by 7 publications

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“…Apart from affecting the health of infected cattle, S. cruzi is a potentially important cause of food poisoning. For instance, Sarcocystis -associated food poisoning cases have been reported in Japan following the consumption of raw Sarcocystis -infected Shika-deer meat [ 2 , 3 , 16 , 25 , 34 ]. In addition, the rabbit ileal loop test, a standard method for the measurement of enterotoxicity, has been reported to demonstrate the enterotoxicity of S. cruzi cyst extracts [ 30 ].…”

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confidence: 99%

Development of a new quantification method of <i>Sarcocystis cruzi</i> through detection of the acetyl-CoA synthetase gene

Doi

Oba

Furuya

et al. 2023

The Journal of Veterinary Medical Science

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Sarcocystis cruzi is a member of the genus Sarcocystis, infecting bovine animals such as cattle and bison as intermediate hosts, and canids such as dogs and raccoon dogs as definitive hosts. Acute sarcocystosis of S. cruzi causes occasional symptoms in cattle, including weight loss, reduced milk production, abortions, and death, and similar to other Sarcocystis species can potentially cause food poisoning in humans when raw or undercooked infected cattle meat is consumed. Despite these issues, genetic information on S. cruzi is scarce, and there is no specific quantitative method for the detection and quantification of the parasite in infected cattle. In this study, we aimed to develop a method based on high-throughput sequencing of S. cruzi genome and transcriptome that specifically and quantitatively detects the S. cruzi acetyl-CoA synthetase gene (ScACS). Cardiac muscles were collected from slaughterhouses in Saitama Prefecture to obtain sarcocysts from which DNA and RNA were extracted for the high-throughput sequencing. Using the sequences, we developed a specific quantitative PCR assay which could distinguish S. cruzi ACS from that of Toxoplasma gondii by taking advantage of the differences in their exon/intron organizations and validated the assay with the microscopic counting of the S. cruzi bradyzoites. Thus, this assay will be useful for future studies of S. cruzi pathogenesis in cattle and for the surveillance of infected animals, thereby easing public health concerns.

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confidence: 99%

Development of a new quantification method of <i>Sarcocystis cruzi</i> through detection of the acetyl-CoA synthetase gene

Doi

Oba

Furuya

et al. 2023

The Journal of Veterinary Medical Science

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Presence of Sarcocystis sybillensis in Japanese sika deer (Cervus nippon) captured in its native territory and its phylogenetic relationship with Sarcocystis nipponi

et al. 2020

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Establishment of a Novel Molecular Detection Method for Sarcocystis in Venison

Yamazaki

Shirafuji

Kamata

2021

Foodborne Pathogens and Disease

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An Outbreak of Suspected Food Poisoning Related to Consumption of Undercooked Deer Meat in Shiga Prefecture

Cited by 7 publications

References 6 publications

Development of a new quantification method of <i>Sarcocystis cruzi</i> through detection of the acetyl-CoA synthetase gene

Development of a new quantification method of <i>Sarcocystis cruzi</i> through detection of the acetyl-CoA synthetase gene

Presence of Sarcocystis sybillensis in Japanese sika deer (Cervus nippon) captured in its native territory and its phylogenetic relationship with Sarcocystis nipponi

Establishment of a Novel Molecular Detection Method for Sarcocystis in Venison

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