An Outbreak of NDM-1-Producing<i>Klebsiella pneumoniae</i>, Associated with OmpK35 and OmpK36 Porin Loss in Tunisia

Hamzaoui, Zaineb; Ocampo-Sosa, Alain A.; Maamar, Elaa; Martínez, Marta Fernández; Ferjani, Sana; Hammami, Samia; Harbaoui, Sarra; Genel, Nathalie; Arlet, Guillaume; Saïdani, Mabrouka; Slim, Aminé; Boubaker, Ilhem Boutiba-Ben; Martı́nez-Martı́nez, Luis

doi:10.1089/mdr.2017.0165

Cited by 41 publications

(34 citation statements)

References 39 publications

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“…Loss of porins is one of the factors contributing to antimicrobial resistance and may favor the selection of additional mechanisms of resistance. OmpK35 and OmpK36 are especially related to resistance to ␤-lactams and quinolones (104).…”

Section: Porin Detectionmentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for the Rapid Detection of Antimicrobial Resistance Mechanisms and Beyond

2018

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SUMMARY Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has been successfully applied in recent years for first-line identification of pathogens in clinical microbiology because it is simple to use, rapid, and accurate and has economic benefits in hospital management. The range of clinical applications of MALDI-TOF MS for bacterial isolates is increasing constantly, from species identification to the two most promising applications in the near future: detection of antimicrobial resistance and strain typing for epidemiological studies. The aim of this review is to outline the contribution of previous MALDI-TOF MS studies in relation to detection of antimicrobial resistance and to discuss potential future challenges in this field. Three main approaches are ready (or almost ready) for clinical use, including the detection of antibiotic modifications due to the enzymatic activity of bacteria, the detection of antimicrobial resistance by analysis of the peak patterns of bacteria or mass peak profiles, and the detection of resistance by semiquantification of bacterial growth in the presence of a given antibiotic. This review provides an expert guide for MALDI-TOF MS users to new approaches in the field of antimicrobial resistance detection, especially possible applications as a routine diagnostic tool in microbiology laboratories.

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Section: Porin Detectionmentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for the Rapid Detection of Antimicrobial Resistance Mechanisms and Beyond

2018

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“…Both global and regional surveillance of drug resistance is fundamental for the implementation of adequate infection control measures and disease management [3]. For this reason, some research groups from Mexico have reported the drug resistance rates of some bacterial pathogens, including Enterobacter sp., Klebsiella pneumoniae , Acinetobacter baumannii , Pseudomonas aeruginosa , Staphylococcus aureus , and Enterococcus faecium [4–7]; with species producing extended-spectrum beta-lactamases (ESBLs) or carbapenemases receiving special consideration [8–13].…”

Section: Introductionmentioning

confidence: 99%

A snapshot of antimicrobial resistance in Mexico. Results from 47 centers from 20 states during a six-month period

Garza‐González¹,

Morfín‐Otero²,

Mendoza-Olazarán³

et al. 2019

PLoS ONE

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AimWe aimed to assess the resistance rates of antimicrobial-resistant, in bacterial pathogens of epidemiological importance in 47 Mexican centers.Material and methodsIn this retrospective study, we included a stratified sample of 47 centers, covering 20 Mexican states. Selected isolates considered as potential causatives of disease collected over a 6-month period were included. Laboratories employed their usual methods to perform microbiological studies. The results were deposited into a database and analyzed with the WHONET 5.6 software.ResultsIn this 6-month study, a total of 22,943 strains were included. Regarding Gram-negatives, carbapenem resistance was detected in ≤ 3% in Escherichia coli, 12.5% in Klebsiella sp. and Enterobacter sp., and up to 40% in Pseudomonas aeruginosa; in the latter, the resistance rate for piperacillin-tazobactam (TZP) was as high as 19.1%. In Acinetobacter sp., resistance rates for cefepime, ciprofloxacin, meropenem, and TZP were higher than 50%. Regarding Gram-positives, methicillin resistance in Staphylococcus aureus (MRSA) was as high as 21.4%, and vancomycin (VAN) resistance reached up to 21% in Enterococcus faecium. Acinetobacter sp. presented the highest multidrug resistance (53%) followed by Klebsiella sp. (22.6%) and E. coli (19.4%).ConclusionThe multidrug resistance of Acinetobacter sp., Klebsiella sp. and E. coli and the carbapenem resistance in specific groups of enterobacteria deserve special attention in Mexico. Vancomycin-resistant enterococci (VRE) and MRSA are common in our hospitals. Our results present valuable information for the implementation of measures to control drug resistance.

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“…The most frequently prescribed carbapenems include ertapenem, imipenem, and meropenem. Unlike other b-lactam antibiotics, carbapenems have the broadest antibacterial spectrum (2).…”

mentioning

confidence: 99%

“…The use of carbapenems for MDR bacterial infections created a selection pressure within the clinical setting, resulting in the emergence of carbapenem resistance (2). Carbapenem resistance is primarily mediated by carbapenemase genes found on mobile genetic elements such as plasmids, integrons, insertion sequences, and transposons, allowing for easier horizontal transfer of genes across and within different bacterial species (9)(10)(11)(12).…”

mentioning

confidence: 99%

Emerging Transcriptional and Genomic Mechanisms Mediating Carbapenem and Polymyxin Resistance in Enterobacteriaceae : a Systematic Review of Current Reports

et al. 2020

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An Outbreak of NDM-1-ProducingKlebsiella pneumoniae, Associated with OmpK35 and OmpK36 Porin Loss in Tunisia

Abstract: This study describes an outbreak of NDM-1-producing K. pneumoniae strains, isolated from a Tunisian hospital, caused by two clones belonging to ST147 and ST307; and highlights the role of OMPs loss, in combination with β-lactamase expression, in conferring high carbapenem resistance.

Cited by 41 publications

References 39 publications

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for the Rapid Detection of Antimicrobial Resistance Mechanisms and Beyond

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for the Rapid Detection of Antimicrobial Resistance Mechanisms and Beyond

A snapshot of antimicrobial resistance in Mexico. Results from 47 centers from 20 states during a six-month period

Emerging Transcriptional and Genomic Mechanisms Mediating Carbapenem and Polymyxin Resistance in Enterobacteriaceae : a Systematic Review of Current Reports

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