An Outbreak of Multiresistant <i>Acinetobacter baumanii</i> in a University Hospital in São Paulo, Brazil

Levin, Anna Sara Shafferman; Mendes, Caio; Sinto, Sumiko I.; Sader, Hélio S.; Scarpitta, Candida R. M.; Rodrigues, Edna; Sauaia, Naim; Boulos, Marcos

doi:10.1086/647319

Cited by 52 publications

(45 citation statements)

References 11 publications

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“…Numerous outbreaks of nosocomial infections caused by multiresistant A. baumannii have been reported in various countries, including Brazil (Oliveira et al 1993, Bergogne-Bérézin & Towner 1996, Levin et al 1996, Vaz et al 1996. Important risk factors are involved in the urinary tract infections, which occur mostly in elderly debilitated patients confined to ICU, and with permanent urinary catheters.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of adherence, hemagglutination, and presence of genes codifying for virulence factors of Acinetobacter baumannii causing urinary tract infection

Braun

Vidotto

2004

Mem. Inst. Oswaldo Cruz

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Section: Resultsmentioning

confidence: 99%

Evaluation of adherence, hemagglutination, and presence of genes codifying for virulence factors of Acinetobacter baumannii causing urinary tract infection

Braun

Vidotto

2004

Mem. Inst. Oswaldo Cruz

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“…This gene is intrinsic to the species and has been identifi ed in a wide collection of isolates of the A. calcoaceticus-A. baumannii complex collected from various geographic areas (13) (28) (29) (31) (32) . Thus, phenotypically identifi ed isolates that possess the bla OXA-51-like gene were considered members of the complex in the present study.…”

Section: Discussionmentioning

confidence: 99%

The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting

Kobs¹,

Ferreira

Bobrowicz

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction: Members of the Acinetobacter genus are key pathogens that cause healthcare-associated infections, and they tend to spread and develop new antibiotic resistance mechanisms. Oxacillinases are primarily responsible for resistance to carbapenem antibiotics. Higher rates of carbapenem hydrolysis might be ascribed to insertion sequences, such as the ISAba1 sequence, near bla OXA genes. The present study examined the occurrence of the genetic elements bla OXA and ISAba1 and their relationship with susceptibility to carbapenems in clinical isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. Methods: Isolates identifi ed over 6 consecutive years in a general hospital in Joinville, Southern Brazil, were evaluated. The investigation of 5 families of genes encoding oxacillinases and the ISAba1 sequence location relative to bla OXA genes was conducted using polymerase chain reaction. Results: All isolates presented the bla OXA-51-like gene (n = 78), and 91% tested positive for the bla OXA-23-like gene (n = 71). The presence of ISAba1 was exclusively detected in isolates carrying the bla OXA-23-like gene. All isolates in which ISAba1 was found upstream of the bla OXA-23-like gene (n = 69) showed resistance to carbapenems, whereas the only isolate in which ISAba1 was not located near the bla OXA-23-like gene was susceptible to carbapenems. The ISAba1 sequence position of another bla OXA-23-like -positive isolate was inconclusive. The isolates exclusively carrying the bla OXA-51-like gene (n = 7) showed susceptibility to carbapenems. Conclusions: The presence of the ISAba1 sequence upstream of the bla OXA-23-like gene was strongly associated with carbapenem resistance in isolates of the A. calcoaceticus-A. baumannii complex in the hospital center studied.

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“…baumannii complex has been responsible for nosocomial infection outbreaks in Europe since 1980, mainly in England, France, Germany, Italy, Spain, and the Netherlands (16) . In Brazilian hospitals, infections have been reported since 1996, mainly at ICUs (17) . Carbapenems became the primary therapeutic options in various hospitals, but the continued use of these antimicrobials has been compromised by the emergence of novel resistance mechanisms (1) .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance profiles and oxacillinase genes in carbapenem-resistant Acinetobacter baumannii isolated from hospitalized patients in Santa Catarina, Brazil

Cortivo¹,

Gutberlet

Ferreira

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Introduction:Carbapenems are the therapy of choice for treating severe infections caused by the Acinetobacter calcoaceticusAcinetobacter baumannii complex. We aimed to assess the prevalence and antimicrobial susceptibility profi les of producers of distinct oxacillinases among nosocomial isolates of the A. calcoaceticus-A. baumannii complex in a 249-bed general hospital located in Joinville, Southern Brazil. Methods: Of the 139 A. baumannii clinical isolates with reduced susceptibility to carbapenems between 2010 and 2013, 118 isolates from varying anatomical sites and hospital sectors were selected for genotypic analysis. Five families of genes encoding oxacillinases, namely bla OXA-23-like , bla OXA-24-like , bla OXA-51-like , bla OXA-58-like , and bla OXA-143-like , were investigated by multiplex polymerase chain reaction (PCR). Results: Most (87.3%) isolates simultaneously carried the bla OXA-23-like and bla OXA-51-like genes, whereas three (2.5%) isolates harbored only bla OXA-51-like ones. The circulation of carbapenemresistant isolates increased during the study period: from none in 2010, to 22 in 2011, 64 in 2012, and 53 in 2013. Conclusions: Isolates carrying the bla OXA-23-like and bla OXA-51-like genes were widely distributed in the hospital investigated. Because of the worsening scenario, the implementation of preventive measures and effective barriers is needed.

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An Outbreak of Multiresistant Acinetobacter baumanii in a University Hospital in São Paulo, Brazil

Cited by 52 publications

References 11 publications

Evaluation of adherence, hemagglutination, and presence of genes codifying for virulence factors of Acinetobacter baumannii causing urinary tract infection

Evaluation of adherence, hemagglutination, and presence of genes codifying for virulence factors of Acinetobacter baumannii causing urinary tract infection

The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting

Antimicrobial resistance profiles and oxacillinase genes in carbapenem-resistant Acinetobacter baumannii isolated from hospitalized patients in Santa Catarina, Brazil

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