“…The isolates were confirmed as members of the MBTC by acid alcohol-fast staining by the method described by Kulski et al (Kulski & Pryce, 1996), and by PCR restriction fragment length polymorphism (RFLP) analysis of the gyrB gene through RsaI and SacII restriction enzymes (Huard et al, 2003;Kasai, Ezaki, & Harayama, 2000;Niemann, Harmsen, Ru¨sch-Gerdes, & Richter, 2000). Isolates were mainly obtained from farm animals (cattle and pigs), but some isolates from wildlife were available (fallow deer (Amato et al, 2016) and wild boars) (Tables 2 and 3), were also included in this study. A M. bovis isolate was obtained from a person, the owner of a TB infected herd, who showed suspected clinical symptoms (pneumonia).…”