An osteoinductive surface by adhesive bone morphogenetic protein-2 prepared using the bioorthogonal approach for tight binding of titanium with bone

Ren, Xueli; Tsuji, Hironori; Uchino, Takahiko; Kono, Izumi; Isoshima, Takashi; Okamoto, Akimitsu; Nagaoka, Noriyuki; Ozaki, Toshifumi; Matsukawa, Akihiro; Miyatake, Hideyuki; Ito, Yoshihiro

doi:10.1039/d3tb02838k

J. Mater. Chem. B

2024

DOI: 10.1039/d3tb02838k

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An osteoinductive surface by adhesive bone morphogenetic protein-2 prepared using the bioorthogonal approach for tight binding of titanium with bone

Xueli Ren,

Hironori Tsuji,

Takahiko Uchino

et al.

Abstract: Inspired by mussel adhesive proteins, we aimed to design an adhesive and dimer-forming highly active bone morphogenetic protein-2 (BMP-2) using bioorthogonal chemistry, involving recombinant DNA technology combined with enzymatic modifications,...

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Biointerface engineering through amalgamation of gene technology and site‐specific growth factor conjugation for efficient osteodifferentiation

Wu,

Mo,

Wang

et al. 2024

Biotech & Bioengineering

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The development of bone implants through bioinspired immobilization of growth factors remains a key issue in the generation of biological interfaces, especially in enhancing osteodifferentiation ability. In this study, we developed a strategy for surface functionalization of poly(lactide‐glycolide) (PLGA) and hydroxyapatite (HA) composite substrates through site‐specific conjugation of bone morphogenetic protein 2 containing 3,4‐hydroxyphenalyalanine (DOPA‐BMP2) mediated by tyrosinase and sortase A (SrtA). Firstly, the growth factor BMP2‐LPETG containing LPETG motif was successfully expressed in Escherichia coli through recombinant DNA technology. The excellent binding affinity of binding growth factor (DOPA‐BMP2) was achieved by converting the tyrosine residue (Y) of YKYKY‐GGG peptide into DOPA (X) by tyrosinase, which bound to the substrates. Then its GGG motif was specifically bound to the end of BMP2‐LPETG mediated by SrtA. Therefore, the generated bioactive DOPA‐BMP2/PLGA/HA substrates significantly promoted the osteogenic differentiation of MC3T3‐E1 cells. Thanks to this microbial‐assisted engineering approach, our work presents a facile and highly site‐specific strategy to engineer biomimetic materials for orthopedics and dentistry by effectively delivering growth factors, peptides, and other biomacromolecules.

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Biointerface engineering through amalgamation of gene technology and site‐specific growth factor conjugation for efficient osteodifferentiation

Wu,

Mo,

Wang

et al. 2024

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

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An osteoinductive surface by adhesive bone morphogenetic protein-2 prepared using the bioorthogonal approach for tight binding of titanium with bone

Abstract: Inspired by mussel adhesive proteins, we aimed to design an adhesive and dimer-forming highly active bone morphogenetic protein-2 (BMP-2) using bioorthogonal chemistry, involving recombinant DNA technology combined with enzymatic modifications,...

Cited by 1 publication

References 61 publications

Biointerface engineering through amalgamation of gene technology and site‐specific growth factor conjugation for efficient osteodifferentiation

Biointerface engineering through amalgamation of gene technology and site‐specific growth factor conjugation for efficient osteodifferentiation

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